Preparation of lysocellin beads by fermentation
    1.
    发明授权
    Preparation of lysocellin beads by fermentation 失效
    通过发酵制备溶胶球蛋白珠

    公开(公告)号:US5849550A

    公开(公告)日:1998-12-15

    申请号:US497397

    申请日:1995-06-30

    IPC分类号: C12P17/16 C12P17/18

    CPC分类号: C12P17/162

    摘要: A method for producing lysocellin in bead form comprises: (a) cultivating a lysocellin-producing microorganism in an aqueous nutrient-containing fermentation broth under lysocellin-producing conditions; (b) adding a base or inorganic phosphate to the fermentation broth within about the first 24 hours of the beginning of the fermentation in an amount sufficient to ensure that the lysocellin is produced in the form of a cation salt of lysocellin; (c) providing a lipid to the fermentation broth such that the lipid is present in the broth in a sufficient amount to form discrete beads with the lysocellin in the fermentation broth; and (d) separating the beads from the broth.

    摘要翻译: 用于制备珠粒形式的溶血细胞素的方法包括:(a)在产生溶血细胞素的条件下,在含水营养的发酵液中培养产生溶血细胞纤维素的微生物; (b)在发酵开始的最初24小时之内,将发酵液中的碱或无机磷酸盐以足以确保溶血细胞素的阳离子盐形式产生的量的量加入到发酵液中; (c)向发酵液提供脂质,使得脂肪以足够的量存在于肉汤中,以在发酵液中与溶菌酶一起形成离散的珠粒; 和(d)从培养液中分离珠子。