公开(公告)号：US12281990B2

公开(公告)日：2025-04-22

申请号：US18035938

申请日：2022-02-28

Applicant: Jiangsu University

Inventor： Xuetao Hu ,  Xiaobo Zou ,  Pei Jiang ,  Jiyong Shi ,  Xinai Zhang ,  Xiaowei Huang ,  Zhihua Li ,  Yahui Li ,  Wenting Li ,  Junjun Zhang

IPC: G01N21/84 ,  B01L3/00 ,  C12Q1/32 ,  C12Q1/46 ,  G01N21/64

Abstract: The present invention belongs to the field of organophosphorus pesticide (OP) detection, and relates to a method for detecting an OP by a microfluidic chip based on a fluorescent sensing film. A porous fluorescent sensing film and the microfluidic chip are first constructed. The fluorescent sensing film is fabricated through layer-by-layer self-assembly of a platinum nanoparticle@oxalate-metal-organic framework (MOF) composite and a porous two-dimensional (2D) nanosheet, and has the functions of specifically detecting OPs and blocking macromolecular interferents. The microfluidic chip includes a sample channel, injection channels, reaction tanks, microfluidic channels, a detection tank, and an optical fiber channel, such that sample pretreatment and detection processes are integrated in the chip. An OP detection system is established by combining a portable constant-pressure syringe pump, a laser, a spectrometer, a signal transmitter, and a signal indicator, such that test devices are miniaturized and integrated and the OP detection is standardized.

公开(公告)号：US11378519B2

公开(公告)日：2022-07-05

申请号：US17053070

申请日：2019-09-26

Applicant: Jiangsu University

Inventor： Jiyong Shi ,  Wenting Li ,  Xiaobo Zou ,  Zhihua Li ,  Xiaowei Huang ,  Zhiming Guo ,  Xuetao Hu ,  Nini Liang ,  Yongqiang Shi ,  Haijun Shi ,  Xueping Cui

IPC: G01N21/77

Abstract: Fluorescent test paper for detecting mercury ions is firstly prepared, the fluorescent test paper including a fluorescence development area and a standard fluorescent colorimetric card. With reference to an excited color corresponding to a reference channel in the standard colorimetric card, the color corresponding to a carbon quantum dots distribution area on the fluorescent test paper is observed to check the fluorescence detection environment during detection with the fluorescent test paper. In colorimetric detection of the content of mercury ions, the concentration of Hg2+ is detected based on an excited color, in the standard colorimetric card, that corresponds to a standard mercury ion sample and is closest to a color signal in a copper nanoclusters distribution area on the fluorescent test paper.