Bacterial counting method
    2.
    发明授权

    公开(公告)号:US10655158B2

    公开(公告)日:2020-05-19

    申请号:US15572555

    申请日:2016-03-30

    Abstract: Disclosed is a bacterial counting method, comprising the following steps, S1: preparing a polyaniline/bacterial composite film on the surface of a glassy carbon electrode by electric polymerization; S2: drawing the standard curve of the polyaniline/bacterial composite film modified electrode; and S3: determining the bacterial concentration of the bacteria solution sample to be tested according to the standard curve obtained in step S2. The method does not require the cultivation of the bacteria in the implementation process, such that same takes a short time and is easy to operate; the method uses aniline as the main reagent, and the consumption of the solution to be tested is small, such that the testing cost is low and the equipment is simple; the method has the advantages of a good repeatability and a wide detection linear range; and the method is an easy to operate, short time-consuming, and low cost bacterial counting method. The method is a bacterial counting method based on the polyaniline/bacterial composite film, and can achieve rapid and accurate detection of the bacterial thallus concentration.

    Method for detecting organophosphorus pesticide by microfluidic chip based on fluorescent sensing film

    公开(公告)号:US12281990B2

    公开(公告)日:2025-04-22

    申请号:US18035938

    申请日:2022-02-28

    Abstract: The present invention belongs to the field of organophosphorus pesticide (OP) detection, and relates to a method for detecting an OP by a microfluidic chip based on a fluorescent sensing film. A porous fluorescent sensing film and the microfluidic chip are first constructed. The fluorescent sensing film is fabricated through layer-by-layer self-assembly of a platinum nanoparticle@oxalate-metal-organic framework (MOF) composite and a porous two-dimensional (2D) nanosheet, and has the functions of specifically detecting OPs and blocking macromolecular interferents. The microfluidic chip includes a sample channel, injection channels, reaction tanks, microfluidic channels, a detection tank, and an optical fiber channel, such that sample pretreatment and detection processes are integrated in the chip. An OP detection system is established by combining a portable constant-pressure syringe pump, a laser, a spectrometer, a signal transmitter, and a signal indicator, such that test devices are miniaturized and integrated and the OP detection is standardized.

Patent Agency Ranking