公开(公告)号：US12281990B2

公开(公告)日：2025-04-22

申请号：US18035938

申请日：2022-02-28

Applicant: Jiangsu University

Inventor： Xuetao Hu ,  Xiaobo Zou ,  Pei Jiang ,  Jiyong Shi ,  Xinai Zhang ,  Xiaowei Huang ,  Zhihua Li ,  Yahui Li ,  Wenting Li ,  Junjun Zhang

IPC: G01N21/84 ,  B01L3/00 ,  C12Q1/32 ,  C12Q1/46 ,  G01N21/64

Abstract: The present invention belongs to the field of organophosphorus pesticide (OP) detection, and relates to a method for detecting an OP by a microfluidic chip based on a fluorescent sensing film. A porous fluorescent sensing film and the microfluidic chip are first constructed. The fluorescent sensing film is fabricated through layer-by-layer self-assembly of a platinum nanoparticle@oxalate-metal-organic framework (MOF) composite and a porous two-dimensional (2D) nanosheet, and has the functions of specifically detecting OPs and blocking macromolecular interferents. The microfluidic chip includes a sample channel, injection channels, reaction tanks, microfluidic channels, a detection tank, and an optical fiber channel, such that sample pretreatment and detection processes are integrated in the chip. An OP detection system is established by combining a portable constant-pressure syringe pump, a laser, a spectrometer, a signal transmitter, and a signal indicator, such that test devices are miniaturized and integrated and the OP detection is standardized.