公开(公告)号：US20120083037A1

公开(公告)日：2012-04-05

申请号：US13145756

申请日：2010-01-29

申请人： Joachim H. Wendorff ,  Achim Aigner ,  Ronald Dersch ,  Sabrina Höbel ,  Markus Rudisile

发明人： Joachim H. Wendorff ,  Achim Aigner ,  Ronald Dersch ,  Sabrina Höbel ,  Markus Rudisile

IPC分类号： C12N15/85 ,  B29C47/00

CPC分类号： A61K48/0041 ,  A61K47/6935 ,  A61K47/6937 ,  B82Y5/00 ,  C12N15/111 ,  C12N15/87 ,  C12N2310/14 ,  C12N2320/32

摘要： The invention relates to a non-viral transfection agent comprising polymer/nucleic acid complexes and nanofibers, wherein the polymer/nucleic acid complexes are composed of at least one nucleic acid and at least one cationic polymer. The nanofibers carry the polymer/nucleic acid complexes, wherein the non-viral transfection agent is advantageously produced by means of electrospinning. The cationic polymer is favorably a polyimine or polyethyleneimine and can be modified with one or more hydrophilic polymers coupled thereto. It can also be advantageous to couple the cationic polymer with one or more carbohydrates and/or with a receptor-specific ligand. The nucleic acid is a DNA or an RNA, or a DNA or RNA derivative, advantageously a therapeutically active nucleic acid. The nanofibers are composed of biodegradable, biocompatible polymers. The nanofibers or the entire transfection agent can be provided with a polymer coating. A method for producing a non-viral transfection agent comprises the following steps: providing the polymer/nucleic acid complex, producing a spinning solution containing the polymer/nucleic acid complexes, and electrospinning.

摘要翻译： 本发明涉及包含聚合物/核酸复合物和纳米纤维的非病毒转染剂，其中所述聚合物/核酸复合物由至少一种核酸和至少一种阳离子聚合物组成。 纳米纤维携带聚合物/核酸复合物，其中非病毒转染剂有利地通过静电纺丝制备。 阳离子聚合物有利地是聚亚胺或聚乙烯亚胺，并且可以与一种或多种与其偶联的亲水性聚合物进行改性。 将阳离子聚合物与一种或多种碳水化合物和/或与受体特异性配体偶联也是有利的。 核酸是DNA或RNA，或DNA或RNA衍生物，有利地是治疗活性核酸。 纳米纤维由可生物降解的生物相容性聚合物组成。 纳米纤维或整个转染剂可以提供聚合物涂层。 制备非病毒转染剂的方法包括以下步骤：提供聚合物/核酸复合物，产生含有聚合物/核酸复合物的纺丝溶液和静电纺丝。