公开(公告)号：US10180433B2

公开(公告)日：2019-01-15

申请号：US15243975

申请日：2016-08-23

Applicant: KAOHSIUNG MEDICAL UNIVERSITY

Inventor： Tian-Lu Cheng ,  Steve Roffler ,  Wen-Wei Lin ,  I-Ju Chen

IPC: G01N33/58 ,  G01N33/68 ,  C07K16/00 ,  C07K16/42 ,  C07K14/00

Abstract: The present invention is related to a method for detecting a protein comprising: (1) providing a fusion protein, wherein the fusion protein comprises the protein which is fused with a secondary antibody detected protein tag, wherein the secondary antibody detected protein tag comprises at least one epitope selected from the Fc region of at least one primary antibody which is capable of being detected by a corresponding secondary antibody; (2) contacting the fusion protein with a secondary antibody which binds to the secondary antibody detected protein tag to form a protein/antibody complex; and (3) detecting the protein/antibody complex.

公开(公告)号：US09458243B2

公开(公告)日：2016-10-04

申请号：US14534174

申请日：2014-11-06

Applicant: KAOHSIUNG MEDICAL UNIVERSITY

Inventor： Tian-Lu Cheng ,  Steve Roffler ,  Wen-Wei Lin ,  I-Ju Chen

IPC: C07K16/42 ,  C07K16/00

CPC classification number: G01N33/6803 ,  C07K14/00 ,  C07K16/00 ,  C07K16/4283 ,  C07K2317/52 ,  C07K2319/00 ,  C07K2319/40 ,  G01N33/58 ,  G01N33/6854 ,  G01N2570/00

Abstract: A method of preparing an epitope for a secondary antibody detected protein tag, comprising: constructing a expressing vector, comprising a nucleotide sequence for encoding the secondary antibody detected protein tag comprising at least one epitope selected from at least one primary antibody which is detected by corresponding secondary antibody; expressing the vector in a host cell; and obtaining the secondary antibody detected protein tag which is expressed in the host cell. Further, a method for constructing a protein molecular weight marker ladder, comprising constructing a plurality of recombinant protein tags with different molecular weights, wherein each the recombinant protein tag comprises at least one epitope of primary antibody. Besides, a secondary antibody detected protein tag comprises at least two peptide sequences of epitopes selected from primary antibodies of at least two different species.

Abstract translation: 一种制备二抗检测蛋白质标签的表位的方法，包括：构建表达载体，其包含用于编码第二抗体检测的蛋白质标签的核苷酸序列，所述核苷酸序列包含至少一个选自至少一个第一抗体的表位，所述表位被相应的 二抗; 在宿主细胞中表达载体; 并获得在宿主细胞中表达的二抗检测蛋白质标签。 此外，构建蛋白质分子量标记梯的方法，包括构建多个具有不同分子量的重组蛋白质标签，其中每个重组蛋白质标签包含一抗的至少一个表位。 此外，二次抗体检测的蛋白质标签包含至少两个选自至少两种不同物种的一抗的表位的肽序列。