公开(公告)号：US20150362480A1

公开(公告)日：2015-12-17

申请号：US14513774

申请日：2014-10-14

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Eun Joo SONG ,  Hyunjung LEE ,  Eunmi BAN ,  Eunice EunKyeong KIM ,  Young Sook YOO

IPC: G01N33/50 ,  G01N33/58

CPC classification number: G01N33/68 ,  G01N33/502 ,  G01N2500/02

Abstract: Provided are a method of analyzing ubiquitin-proteasome activity with respect to a target polypeptide and a method of screening a ubiquitin-proteasome inhibitor. According to the provided methods, the pattern of lysis of a target polypeptide by a ubiquitin-proteasome in target cells may be quantitatively analyzed in a rapid and highly sensitive way, a ubiquitin-proteasome inhibitor may be screened in a rapid and highly sensitive way, and an anticancer agent and the activity thereof may be rapidly screened.

Abstract translation: 提供了一种分析相关靶多肽的泛素 - 蛋白酶体活性的方法和筛选泛素 - 蛋白酶体抑制剂的方法。 根据提供的方法，可以以快速和高度敏感的方式定量分析目标细胞中泛素 - 蛋白酶体的目标多肽的裂解模式，可以以快速和高度敏感的方式筛选泛素 - 蛋白酶体抑制剂， 并且可以快速筛选抗癌剂并且其活性。