公开(公告)号：US20140242578A1

公开(公告)日：2014-08-28

申请号：US13865745

申请日：2013-04-18

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Eun Joo SONG ,  Eunmi Ban

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: The present invention relates to a method for simultaneously detecting multiple miRNAs present in a sample and a kit for detecting same. According to the present invention, two or more miRNAs existing in trace amounts in a sample can be analyzed through only one measurement. The detection method of the present invention may be used for fast diagnosis of various diseases wherein miRNAs are involved, for example, cardiovascular diseases including myocardial infarction, with high accuracy.

Abstract translation: 本发明涉及同时检测样品中存在的多个miRNA的方法和用于检测样品的试剂盒。 根据本发明，可以通过仅一次测量来分析样品中痕量存在的两种或更多种miRNA。 本发明的检测方法可以用于快速诊断其中涉及miRNA的各种疾病，例如包括心肌梗死的心血管疾病，具有高精度。

公开(公告)号：US20150344887A1

公开(公告)日：2015-12-03

申请号：US14300590

申请日：2014-06-10

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Eun Joo SONG ,  Eunice EunKyeong Kim ,  Tanuza Das

IPC: C12N15/113

CPC classification number: C12N15/1137 ,  C12N2310/14 ,  C12Y301/02015 ,  C12Y304/19012

Abstract: Disclosed herein is an siRNA that inhibits the expression of USP 15 protein and a pharmaceutical composition containing the same for preventing or treating cancers. According to the present invention, the siRNA of the present invention inhibits the expression of USP 15 protein thereby substantially inhibiting the growth and metastasis of cancer cells.

Abstract translation: 本文公开了抑制USP15蛋白质的表达的siRNA和含有该siRNA的药物组合物，用于预防或治疗癌症。 根据本发明，本发明的siRNA抑制USP15蛋白的表达，从而基本上抑制癌细胞的生长和转移。

公开(公告)号：US20150362480A1

公开(公告)日：2015-12-17

申请号：US14513774

申请日：2014-10-14

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Eun Joo SONG ,  Hyunjung LEE ,  Eunmi BAN ,  Eunice EunKyeong KIM ,  Young Sook YOO

IPC: G01N33/50 ,  G01N33/58

CPC classification number: G01N33/68 ,  G01N33/502 ,  G01N2500/02

Abstract: Provided are a method of analyzing ubiquitin-proteasome activity with respect to a target polypeptide and a method of screening a ubiquitin-proteasome inhibitor. According to the provided methods, the pattern of lysis of a target polypeptide by a ubiquitin-proteasome in target cells may be quantitatively analyzed in a rapid and highly sensitive way, a ubiquitin-proteasome inhibitor may be screened in a rapid and highly sensitive way, and an anticancer agent and the activity thereof may be rapidly screened.

Abstract translation: 提供了一种分析相关靶多肽的泛素 - 蛋白酶体活性的方法和筛选泛素 - 蛋白酶体抑制剂的方法。 根据提供的方法，可以以快速和高度敏感的方式定量分析目标细胞中泛素 - 蛋白酶体的目标多肽的裂解模式，可以以快速和高度敏感的方式筛选泛素 - 蛋白酶体抑制剂， 并且可以快速筛选抗癌剂并且其活性。

公开(公告)号：US20140205998A1

公开(公告)日：2014-07-24

申请号：US13758357

申请日：2013-02-04

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Eun Joo SONG ,  Eunmi BAN ,  Dong-Kyu CHAE

IPC: C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q1/6816 ,  C12Q2525/207 ,  C12Q2527/125

Abstract: The present invention relates to a method for improving detection efficiency of miRNAs existing in cells in trace amounts by adding Triton X-100. In accordance with the present invention, miRNAs existing in a sample in trace amounts can be quantitatively analyzed in short time. Further, the miRNA detection method according to the present invention wherein Triton X-100 is used can improve detection efficiency by about 2 times as compared to when only a TRIzol reagent is used.

Abstract translation: 本发明涉及通过加入Triton X-100来提高存在于微量细胞中的miRNA的检测效率的方法。 根据本发明，可以在短时间内定量分析存在于痕量样品中的miRNA。 此外，与仅使用TRIzol试剂相比，使用Triton X-100的根据本发明的miRNA检测方法可以将检测效率提高约2倍。