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1.发明申请DETECTION OF miRNA USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION 审中-公开使用激光诱导荧光检测的毛细管电泳检测miRNA公开(公告)号：US20140099633A1
公开(公告)日：2014-04-10
申请号：US13682941
申请日：2012-11-21
Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventor： Eunmi BAN , Dong-Kyu Chae , Eun Joo Song
IPC: C12Q1/68
CPC classification number: C12Q1/6883 , C12Q1/6816 , C12Q2600/158 , C12Q2600/178 , C12Q2525/207 , C12Q2565/629
Abstract: Disclosed is a method for detecting a miRNA present in a sample in trace amounts and a kit for detecting the same. According to the present invention, the miRNA present in the sample in trace amounts can be quantitatively analyzed in short time. The detection method of the present invention may be used for fast diagnosis of various diseases wherein miRNAs are involved, for example, cardiovascular diseases including myocardial infarction.
Abstract translation: 公开了一种用于检测微量样品中存在的miRNA的方法和用于检测其的试剂盒。 根据本发明，可以在短时间内定量分析存在于微量样品中的miRNA。 本发明的检测方法可用于快速诊断涉及miRNA的各种疾病，例如包括心肌梗死的心血管疾病。
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2.发明申请METHOD OF ANALYZING UBIQUITIN-PROTEASOME ACTIVITY AND METHOD OF SCREENING UBIQUITIN-PROTEASOME INHIBITOR USING THE SAME 有权分析UBIQUITIN-PROTEASOME活性的方法和使用其筛选UBIQUITIN-PROTEASOME抑制剂的方法公开(公告)号：US20150362480A1
公开(公告)日：2015-12-17
申请号：US14513774
申请日：2014-10-14
Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventor： Eun Joo SONG , Hyunjung LEE , Eunmi BAN , Eunice EunKyeong KIM , Young Sook YOO
CPC classification number: G01N33/68 , G01N33/502 , G01N2500/02
Abstract: Provided are a method of analyzing ubiquitin-proteasome activity with respect to a target polypeptide and a method of screening a ubiquitin-proteasome inhibitor. According to the provided methods, the pattern of lysis of a target polypeptide by a ubiquitin-proteasome in target cells may be quantitatively analyzed in a rapid and highly sensitive way, a ubiquitin-proteasome inhibitor may be screened in a rapid and highly sensitive way, and an anticancer agent and the activity thereof may be rapidly screened.
Abstract translation: 提供了一种分析相关靶多肽的泛素 - 蛋白酶体活性的方法和筛选泛素 - 蛋白酶体抑制剂的方法。 根据提供的方法，可以以快速和高度敏感的方式定量分析目标细胞中泛素 - 蛋白酶体的目标多肽的裂解模式，可以以快速和高度敏感的方式筛选泛素 - 蛋白酶体抑制剂， 并且可以快速筛选抗癌剂并且其活性。
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3.发明申请METHOD FOR ENHANCING EXTRACTION EFFICIENCY OF MIRNA FROM CELLS BY THE ADDITION OF TRITON X-100 审中-公开通过添加TRITON X-100来提高细胞提取MIRNA提取效率的方法公开(公告)号：US20140205998A1
公开(公告)日：2014-07-24
申请号：US13758357
申请日：2013-02-04
Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventor： Eun Joo SONG , Eunmi BAN , Dong-Kyu CHAE
IPC: C12Q1/68
CPC classification number: C12N15/1003 , C12Q1/6816 , C12Q2525/207 , C12Q2527/125
Abstract: The present invention relates to a method for improving detection efficiency of miRNAs existing in cells in trace amounts by adding Triton X-100. In accordance with the present invention, miRNAs existing in a sample in trace amounts can be quantitatively analyzed in short time. Further, the miRNA detection method according to the present invention wherein Triton X-100 is used can improve detection efficiency by about 2 times as compared to when only a TRIzol reagent is used.
Abstract translation: 本发明涉及通过加入Triton X-100来提高存在于微量细胞中的miRNA的检测效率的方法。 根据本发明，可以在短时间内定量分析存在于痕量样品中的miRNA。 此外，与仅使用TRIzol试剂相比，使用Triton X-100的根据本发明的miRNA检测方法可以将检测效率提高约2倍。
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