公开(公告)号：US11261480B2

公开(公告)日：2022-03-01

申请号：US16849833

申请日：2020-04-15

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Geir Fonnum ,  Nini Kjus

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

公开(公告)号：US09909165B2

公开(公告)日：2018-03-06

申请号：US15431678

申请日：2017-02-13

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Geir Fonnum ,  Nini Kjus

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support while maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

公开(公告)号：US20200308630A1

公开(公告)日：2020-10-01

申请号：US16849833

申请日：2020-04-15

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Geir Fonnum ,  Nini Kjus

IPC: C12Q1/6806 ,  C12N15/10

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

公开(公告)号：US10787659B2

公开(公告)日：2020-09-29

申请号：US16137448

申请日：2018-09-20

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Hannah Lindstroem

IPC: C12N15/10

Abstract: The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves mixing dibromochloromethane, iodochloromethane or mixtures thereof with a biological sample that has been mixed with a phenol-based extraction solution. Centrifugation of the sample is not needed to achieve phase separation which occurs using the present methods in as little as one to two minutes with no phenol carryover.

公开(公告)号：USRE46207E1

公开(公告)日：2016-11-22

申请号：US29454427

申请日：2013-05-09

Applicant: LIFE TECHNOLOGIES AS

Inventor： Gaute Brede ,  Darren Ellis ,  Tine Hildisch ,  Jeremy (“Jez”) Mundy ,  Lars Nokleby ,  Erlend Ragnhildstveit ,  Richard Toften ,  Kornelija Zgonc