-
1.
公开(公告)号:US20190233810A1
公开(公告)日:2019-08-01
申请号:US16312212
申请日:2017-06-30
IPC分类号: C12N15/10
CPC分类号: C12N15/1013 , C12N15/1006
摘要: The present invention relates to improved methods of isolating nucleic acids. In particular the method comprises the use of a wash buffer comprising bivalent cations prior to elution of the nucleic acid.
-
2.
公开(公告)号:US20180267036A1
公开(公告)日:2018-09-20
申请号:US15993468
申请日:2018-05-30
发明人: Christina Fan , Olaf Zoellner
IPC分类号: G01N33/566 , G06F19/10 , G01N33/68 , G01N33/543 , G01N33/50 , C12Q1/68 , C12N15/10 , C12Q1/6806 , C12Q1/6823 , C12Q1/6869 , G06F19/00
CPC分类号: G01N33/566 , B01J2219/00646 , B01J2219/00722 , B01J2219/00725 , C07K2319/01 , C12N15/1006 , C12N15/1096 , C12Q1/68 , C12Q1/6804 , C12Q1/6806 , C12Q1/6823 , C12Q1/6869 , G01N33/505 , G01N33/54306 , G01N33/54313 , G01N33/6842 , G01N33/6857 , G06F19/00 , G16B25/10 , G16B99/00 , G16H10/20 , G16H20/13 , C12Q2525/161 , C12Q2525/173 , C12Q2563/179 , C12Q2565/514
摘要: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.
-
公开(公告)号:US20180245064A1
公开(公告)日:2018-08-30
申请号:US15757323
申请日:2016-09-02
申请人: Qiagen Sciences LLC
发明人: Vince MORONEY , Eddie ADAMS , Mark N. BROLASKI , Ingemar PEDRON
IPC分类号: C12N15/10
CPC分类号: C12N15/101 , C12N15/1003 , C12N15/1006 , C12N15/1013 , C12N15/1017
摘要: Provided are methods and compositions for negatively and positively selecting for different size nucleic acid (e.g., DNA or RNA) fragments on borosilicate glass fiber membranes, silica and metal oxide surfaces such that only those fragments falling within a desired size range are obtained.
-
公开(公告)号:US10053731B2
公开(公告)日:2018-08-21
申请号:US15696469
申请日:2017-09-06
发明人: Paul Blainey , Soohong Kim
IPC分类号: B01L3/00 , C12Q1/6874 , B01D35/02 , B01L7/00 , C12N15/10 , C12Q1/6806 , F16K99/00
CPC分类号: C12Q1/6874 , B01D35/02 , B01L3/502707 , B01L3/502715 , B01L3/502738 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/0668 , B01L2200/10 , B01L2300/021 , B01L2300/0663 , B01L2300/0861 , B01L2300/0867 , B01L2300/0887 , B01L2300/123 , B01L2400/0409 , B01L2400/0487 , B01L2400/06 , B01L2400/0622 , B01L2400/0655 , C12N15/1006 , C12Q1/6806 , F16K99/0015 , F16K99/0026 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0084
摘要: The invention generally provides a sieve valve including: a substrate defining a channel; a flexible membrane adapted and configured for deployment at an intersection with the channel; and one or more protrusions extending into the channel from the substrate or the flexible membrane. The one or more protrusions define a plurality of recesses extending beyond the intersection between the channel and the flexible membrane;A microfluidic circuit including one or more sieve valves. In particular embodiments, the circuit comprises one or more input/output valves. The one or one or more input/output valves can include one or more input valves and one or more output valves.The microfluidic circuit can further include a mixing circuit. At least one of the sieve valves can be positioned between the one or more input/output valves and the mixing circuit.The invention further provides methods of using the device for the analysis of samples comprising cells.
-
5.
公开(公告)号:US20180201922A1
公开(公告)日:2018-07-19
申请号:US15917010
申请日:2018-03-09
申请人: Kaneka Corporation
IPC分类号: C12N15/10
CPC分类号: C12N15/1006 , C12M1/00 , C12M1/26 , C12N15/00 , C12Q1/24 , G01N2333/35
摘要: A method for separating a nucleic acid from a specimen includes accommodating a specimen in an accommodation space provided in a nucleic acid-collecting member, transferring a treatment reagent to the nucleic acid-collecting member by connecting a treatment reagent-accommodating container to a treatment reagent-supplying opening of the nucleic acid-collecting member, obtaining a mixture by mixing the specimen and the treatment reagent in a mixing space formed by combining an inner container space of the treatment reagent-accommodating container and the accommodation space of the nucleic acid-collecting member, and pressure-feeding the mixture by reducing a volume of the inner container space of the treatment reagent-accommodating container and discharging the mixture from an mixture-discharging opening of the nucleic acid-collecting member via a carrier that adsorbs the nucleic acid released in the mixture while allowing the mixture to permeate therethrough.
-
公开(公告)号:US20180148766A1
公开(公告)日:2018-05-31
申请号:US15568471
申请日:2016-02-26
申请人: AJ INNUSCREEN GMBH
发明人: Timo Hillebrand , Thorsten Stroh
IPC分类号: C12Q1/6806 , B01L3/02 , G01N1/40
CPC分类号: C12N15/1006 , B01L3/0275 , B01L2200/023 , B01L2200/0631 , B01L2200/0647 , B01L2300/0854 , B01L2300/16 , C12N15/1013 , C12Q1/6806 , G01N1/405 , G01N35/10
摘要: Device and method for automated extraction of nucleic acids, comprising a body that can be immersed partly or completely in a reaction cavity, characterized in that at least the part immersed in the reaction cavity has a non-smooth surface. After lysis, an organic substance, preferably alcohols or ketones, is mixed with a biological sample. This mixture is now brought into contact with a material characterized by a non-smooth surface. Under these conditions, nucleic acids are adsorbed on the surface of the material being used. Thereupon washing steps with known alcoholic washing solutions may be carried out. After drying, the adsorbed nucleic acid is detached from the material by addition of water or a buffer of low salt concentration, whereupon it can be used for downstream applications.
-
公开(公告)号:US20180094254A1
公开(公告)日:2018-04-05
申请号:US15728199
申请日:2017-10-09
IPC分类号: C12N15/10
CPC分类号: C12N15/101 , C12N15/1003 , C12N15/1006 , C12N15/1013 , C12N15/1017
摘要: Provided herein is technology related to processing samples of nucleic acids and particularly, but not exclusively, to methods for enriching samples for small nucleic acids, such as small circulating cell-free DNA.
-
公开(公告)号:US20180087078A1
公开(公告)日:2018-03-29
申请号:US15566904
申请日:2016-04-15
发明人: David A. Weitz , Huidan Zhang , Hohn Heyman , Allon Moshe Klein
IPC分类号: C12P19/34 , C12N15/10 , C12Q1/6869
CPC分类号: C12P19/34 , C12N15/1006 , C12N15/1065 , C12N15/1093 , C12Q1/6869 , C12Q2600/156 , C12Q2563/159 , C12Q2565/514 , C12Q2523/319 , C12Q2531/113 , C12Q2535/122 , C12Q2563/179
摘要: The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.
-
公开(公告)号:US20180087049A1
公开(公告)日:2018-03-29
申请号:US15698326
申请日:2017-09-07
发明人: Wai-Bing Mak , Dominik Duelli , Shihai Huang
CPC分类号: C12N15/1006 , C12N15/1013 , C12Q1/6806 , C12Q1/706 , C12Q2527/125
摘要: The invention provides methods for isolating DNA from a blood sample collected into tubes containing a rapid clot activator, which involves the use of a lysis buffer comprising an alcohol.
-
公开(公告)号:US09896683B2
公开(公告)日:2018-02-20
申请号:US14812694
申请日:2015-07-29
CPC分类号: C12N15/1013 , C12N15/1006 , C12N15/1017 , C12Q1/6806 , C12Q1/6883 , C12Q2600/158 , C12Q2600/178 , C12Q2527/125
摘要: Methods for isolating circulating small RNAs, e.g., microRNA (miRNA), from plasma samples, e.g., that comprise using an alkaline phenol:chloroform extraction, and methods of use thereof, including for the detection, prognosis, and/or monitoring of disease in a subject.
-
-
-
-
-
-
-
-
-