公开(公告)号：US20220251669A1

公开(公告)日：2022-08-11

申请号：US17658493

申请日：2022-04-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shrutii SARDA ,  Anna MCGEACHY ,  Rajesh GOTTIMUKKALA ,  David MERRILL ,  Heesun SHIN ,  Aren EWING ,  Wing LEE ,  Birgit DREWS ,  Fiona HYLAND ,  Janice AU-YOUNG

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/686 ,  G16B30/10

Abstract: The present disclosure provides compositions and methods, as well as combinations, kits, and systems that include the compositions and methods, for amplification, detection, characterization, assessment, profiling and/or measurement of nucleic acids in samples, particularly biological samples. Compositions and methods provided herein include combinations of microbial species target-specific nucleic acid primers for selective amplification and/or combinations of primers for amplification of nucleic acids from a large group of taxonomically related microorganisms. In one aspect, amplified nucleic acids obtained using the compositions and methods can be used in various processes including nucleic acid sequencing and used to detect the presence of microbial species and assess microbial populations in a variety of samples. In accordance with the teachings and principles, new methods, systems and non-transitory machine-readable storage medium are provided to compress reference sequence databases used in mapping sequence reads for analysis and profiling of microbial populations.

公开(公告)号：US20210062271A1

公开(公告)日：2021-03-04

申请号：US16948046

申请日：2020-08-28

Applicant: Life Technologies Corporation

Inventor： David CHI ,  Aren EWING ,  Na LI ,  Zunping LUO ,  Amir MARCOVITZ

IPC: C12Q1/6886

Abstract: Provided are methods and compositions for preparing a library of target nucleic acid sequences that are useful for assessing gene mutations for oncology biomarker profiling of samples. In particular, a target-specific primer panel is provided that allows for selective amplification of oncology biomarker target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with oncology biomarkers from two or more sample types. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants of one or more targeted sequences associated with oncology.