公开(公告)号：US20210254181A1

公开(公告)日：2021-08-19

申请号：US17301976

申请日：2021-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David MERRILL ,  Pius BRZOSKA ,  Zheng LI ,  Wendy LIN ,  Wing LEE ,  Mandi WONG

IPC: C12Q1/6886 ,  C12Q1/6858 ,  C12Q1/6851

Abstract: A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of SEQ ID NO:1-38, such as SEQ ID NO:1-13. Also disclosed are kits and compositions, each comprising a first probe which specifically hybridizes to at least a portion of at least one reference sequence. Also disclosed is a system configured to perform the above method.

公开(公告)号：US20170096711A1

公开(公告)日：2017-04-06

申请号：US15128076

申请日：2015-03-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David MERRILL ,  Pius BRZOSKA ,  Zheng LI ,  Wendy LIN ,  Wing LEE ,  Mandi WONG

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/166 ,  C12Q2537/16 ,  C12Q2543/10 ,  C12Q2545/101 ,  C12Q2547/107 ,  C12Q2561/101

Abstract: A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of SEQ ID NO:1-38, such as SEQ ID NO:1-13. Also disclosed are kits and compositions, each comprising a first probe which specifically hybridizes to at least a portion of at least one reference sequence. Also disclosed is a system configured to perform the above method.

公开(公告)号：US20240263247A1

公开(公告)日：2024-08-08

申请号：US18635799

申请日：2024-04-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David MERRILL ,  Pius BRZOSKA ,  Zheng LI ,  Wendy LIN ,  Wing LEE ,  Mandi WONG

IPC: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6858

CPC classification number: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/166

Abstract: Disclosed herein is a method including one or more of amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence can have at least 80% sequence identity to at least one of SEQ ID NO:1-38. Also disclosed are kits, systems and compositions that can include a first probe which specifically hybridizes to at least a portion of at least one reference sequence.

公开(公告)号：US20220251669A1

公开(公告)日：2022-08-11

申请号：US17658493

申请日：2022-04-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shrutii SARDA ,  Anna MCGEACHY ,  Rajesh GOTTIMUKKALA ,  David MERRILL ,  Heesun SHIN ,  Aren EWING ,  Wing LEE ,  Birgit DREWS ,  Fiona HYLAND ,  Janice AU-YOUNG

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/686 ,  G16B30/10

Abstract: The present disclosure provides compositions and methods, as well as combinations, kits, and systems that include the compositions and methods, for amplification, detection, characterization, assessment, profiling and/or measurement of nucleic acids in samples, particularly biological samples. Compositions and methods provided herein include combinations of microbial species target-specific nucleic acid primers for selective amplification and/or combinations of primers for amplification of nucleic acids from a large group of taxonomically related microorganisms. In one aspect, amplified nucleic acids obtained using the compositions and methods can be used in various processes including nucleic acid sequencing and used to detect the presence of microbial species and assess microbial populations in a variety of samples. In accordance with the teachings and principles, new methods, systems and non-transitory machine-readable storage medium are provided to compress reference sequence databases used in mapping sequence reads for analysis and profiling of microbial populations.