公开(公告)号：US20150225774A1

公开(公告)日：2015-08-13

申请号：US14603076

申请日：2015-01-22

Applicant: Life Technologies Corporation

Inventor： Maxim G. BREVNOV ,  Elena BOLCHAKOVA ,  Manohar FURTADO

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6869 ,  C12Q2563/131 ,  C12Q2563/143

Abstract: A method for preparing a nucleic acid sample for nucleic acid sequencing includes amplifying a nucleic acid target sequence using a primer bound to a first capture substrate; capturing an amplified nucleic acid product by the first capture substrate; generating at least one sequencing ladder from the amplified nucleic acid product using at least one sequencing primer; capturing the at least one sequencing ladder by hybridizing the at least one sequencing ladder to a complementary capture compound on a second capture substrate; and removing the at least one sequencing ladder from the second capture substrate. The first and/or second capture substrate may include a magnetic particle. Other methods, workflows, kits, and computer program media for nucleic acid sample preparation are also disclosed.

Abstract translation: 用于制备用于核酸测序的核酸样品的方法包括使用与第一捕获底物结合的引物扩增核酸靶序列; 通过第一捕获基质捕获扩增的核酸产物; 使用至少一个测序引物从扩增的核酸产物产生至少一个测序梯; 通过将所述至少一个测序梯度杂交到第二捕获基底上的互补捕获化合物来捕获所述至少一个测序梯; 以及从所述第二捕获基板去除所述至少一个测序梯。 第一和/或第二捕获基板可以包括磁性颗粒。 还公开了用于核酸样品制备的其它方法，工作流程，试剂盒和计算机程序培养基。

公开(公告)号：US20150307869A1

公开(公告)日：2015-10-29

申请号：US14697962

申请日：2015-04-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kevin HACKER ,  Gregory GOVONI ,  Nikolay SERGEEV ,  Elena BOLCHAKOVA ,  Maxim BREVNOV ,  Manohar FURTADO ,  Johnie YOUNG ,  James NURSE ,  Mariela CUADRAS

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  B01D29/13 ,  C12N1/066 ,  C12Q1/68 ,  C12Q1/689 ,  C12Q1/6895 ,  G01N21/75

Abstract: Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.

Abstract translation: 提供了用于检测诸如饮用水，药物溶液和组织培养基的流体中的微生物污染物如细菌和真菌的装置和方法。 更具体地，提供了用于捕获和处理来自流体的微生物的过滤装置，以及基于物理破坏与小珠和裂解溶液的组合的微生物的回收，裂解和检测的改进方法。