公开(公告)号：US20150142323A1

公开(公告)日：2015-05-21

申请号：US13261898

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Wessel ,  Jamie Cho ,  Howard Weiss ,  Eric Van Helene ,  Thomas B. Morrison

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  G16B40/00 ,  G16B45/00

Abstract: According to one exemplary embodiment, a method for providing a amplification quality metric to a user is provided. The method includes receiving amplification data from an amplification of a sample to generate an amplification curve. The amplification curve includes an exponential region and a transition region. The method further includes determining a first value of the transition region and determining a second value of the transition region. The first value is the beginning of the transition region and the second value is the end of the transition region. Next, the amplification quality metric is calculated based on at least the first value and the second value. Then, the amplification quality metric is displayed to the user.

Abstract translation: 根据一个示例性实施例，提供了一种用于向用户提供放大质量度量的方法。 该方法包括从样品的扩增接收扩增数据以产生扩增曲线。 扩增曲线包括指数区域和过渡区域。 该方法还包括确定过渡区域的第一值并确定过渡区域的第二值。 第一个值是过渡区域的开始，第二个值是过渡区域的结束。 接下来，基于至少第一值和第二值来计算放大质量度量。 然后，向用户显示放大质量度量。