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    METHODS AND SYSTEMS FOR INSTRUMENT VALIDATION
    2.
    发明申请
    METHODS AND SYSTEMS FOR INSTRUMENT VALIDATION 审中-公开
    仪器验证的方法和系统

    公开(公告)号：US20160231245A1

    公开(公告)日：2016-08-11

    申请号：US15016485

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Thomas Wessel ,  Yong Chu ,  Jacob Freudenthal ,  David Woo

    IPC: G01N21/64

    CPC classification number: G01N21/6428 ,  G01N21/274 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G16B40/00

    Abstract: In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (Ct) values of amplification curves generated from the samples of the first quantity and a second set of Ct values of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Ct values at each of the plurality of fluorescence thresholds.

    Abstract translation: 在一个示例性实施例中，提供了用于验证仪器的方法。 该方法包括从验证板接收放大数据以产生多个扩增曲线。 验证板包括第一数量和第二数量的样本，并且每个扩增曲线包括指数区域。 该方法还包括基于多个扩增曲线的指数区域来确定一组荧光阈值，并且针对该组的每个荧光阈值确定从样品中产生的扩增曲线的第一组循环阈值（Ct）值 从第二数量的样本产生的第一数量和第二组扩增曲线的Ct值。 该方法包括基于多个荧光阈值中的每个荧光阈值处的Ct值来计算第一和第二量是否足够可区分。

    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION
    3.
    发明申请
    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 审中-公开

    公开(公告)号：US20180292320A1

    公开(公告)日：2018-10-11

    申请号：US16010359

    申请日：2018-06-15

    Applicant: Life Technologies Corporation

    Inventor： Yong Chu ,  Jeffrey Marks ,  Jacob Freudenthal ,  Thomas Wessel ,  David Woo

    IPC: G01N21/64 ,  C12Q1/686 ,  G01N21/27 ,  C12Q1/6851

    Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging fluorescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

    Methods and Systems for Streamlining Optical Calibration
    4.
    发明申请
    Methods and Systems for Streamlining Optical Calibration 有权
    简化光学校准的方法和系统

    公开(公告)号：US20140294268A1

    公开(公告)日：2014-10-02

    申请号：US14348206

    申请日：2012-09-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jacob K. Freudenthal ,  Jeffrey A. Marks ,  Francis T. Cheng ,  Thomas Wessel

    IPC: G06T7/00

    CPC classification number: G06T7/0012 ,  G06T5/008 ,  G06T5/50 ,  G06T2207/10024 ,  G06T2207/10064 ,  G06T2207/10144 ,  G06T2207/20021 ,  G06T2207/20216 ,  G06T2207/30072

    Abstract: A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.

    Abstract translation: 提供了一种用于校准生物仪器的方法。 该方法包括以下步骤：获取至少一个生物样本阵列的图像，确定图像内的第一感兴趣区域，其中所述第一感兴趣区域包括所述至少一个生物阵列上的第一多个位置; 以及在所述第一感兴趣区域内识别与所述至少一个生物阵列上的所述第一多个位置中的每一个相关联的多个图像元素。

    Determining the limit of detection of rare targets using digital PCR
    6.
    发明授权
    Determining the limit of detection of rare targets using digital PCR 有权

    公开(公告)号：US11004540B2

    公开(公告)日：2021-05-11

    申请号：US15579407

    申请日：2016-06-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Nivedita Sumi Majumdar ,  Thomas Wessel

    IPC: G16B40/00 ,  G06N7/00

    Abstract: A method for determining false positives calls in a biological data plot is provided. The method includes identifying a first data cluster as non-amplification data points within the biological data plot and identifying a second data cluster as wild-type positives within the biological data plot. The method further includes estimating a position in the biological data plot of a center of the first and second data clusters. The method further includes determining, for each data point within the first data cluster, a probability of belonging to the first data cluster and determining, for each data point within the second data cluster, a probability of belonging to the second data cluster. The method includes applying a probability threshold for each data point within the first and second data cluster to identify false positives.

    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION
    9.
    发明申请
    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 有权
    生物仪器校准的方法和系统

    公开(公告)号：US20160231246A1

    公开(公告)日：2016-08-11

    申请号：US15017249

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Yong Chu ,  Jeffrey Marks ,  Jacob Freudenthal ,  Thomas Wessel ,  David Woo

    IPC: G01N21/64 ,  C12Q1/68

    CPC classification number: G01N21/6428 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/127 ,  G01N2201/13 ,  G01N2333/922

    Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

    Abstract translation: 在一个示例性实施例中，提供了一种用于校准仪器的方法。 该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。 该方法包括执行感兴趣区域（ROI）校准以确定图像中的反应位置位置。 该方法还包括进行纯染料校准，以通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来确定每个反应位点中使用的荧光染料的贡献。 该方法还包括执行仪器归一化校准以确定滤波器归一化因子。 该方法包括执行RNase P验证以验证仪器能够区分两种不同数量的样品。

    METHOD AND SYSTEM FOR DETERMINING AN AMPLIFICATION QUALITY METRIC
    10.
    发明申请
    METHOD AND SYSTEM FOR DETERMINING AN AMPLIFICATION QUALITY METRIC 审中-公开
    用于确定放大质量公差的方法和系统

    公开(公告)号：US20150142323A1

    公开(公告)日：2015-05-21

    申请号：US13261898

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Thomas Wessel ,  Jamie Cho ,  Howard Weiss ,  Eric Van Helene ,  Thomas B. Morrison

    IPC: C12Q1/68

    CPC classification number: C12Q1/6851 ,  G16B40/00 ,  G16B45/00

    Abstract: According to one exemplary embodiment, a method for providing a amplification quality metric to a user is provided. The method includes receiving amplification data from an amplification of a sample to generate an amplification curve. The amplification curve includes an exponential region and a transition region. The method further includes determining a first value of the transition region and determining a second value of the transition region. The first value is the beginning of the transition region and the second value is the end of the transition region. Next, the amplification quality metric is calculated based on at least the first value and the second value. Then, the amplification quality metric is displayed to the user.

    Abstract translation: 根据一个示例性实施例，提供了一种用于向用户提供放大质量度量的方法。 该方法包括从样品的扩增接收扩增数据以产生扩增曲线。 扩增曲线包括指数区域和过渡区域。 该方法还包括确定过渡区域的第一值并确定过渡区域的第二值。 第一个值是过渡区域的开始，第二个值是过渡区域的结束。 接下来，基于至少第一值和第二值来计算放大质量度量。 然后，向用户显示放大质量度量。

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