公开(公告)号：US12226745B2

公开(公告)日：2025-02-18

申请号：US16403339

申请日：2019-05-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham Rosenbaum ,  Collyn Seeger ,  Jeremy Gray ,  Hua Yu

IPC: C12Q1/6853 ,  B01J19/00 ,  C12Q1/6844 ,  C40B50/14

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：US11293056B2

公开(公告)日：2022-04-05

申请号：US16183722

申请日：2018-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham Rosenbaum ,  Collyn Seeger ,  Jeremy Gray ,  Hua Yu

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q1/6874

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：US11268117B2

公开(公告)日：2022-03-08

申请号：US16308803

申请日：2017-06-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Hua Yu ,  Theo Nikiforov ,  Abraham Rosenbaum

IPC: C12P19/34 ,  C12Q1/6853 ,  C12Q1/6844

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related compositions and kits for recombinase-mediated nucleic acid amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using at least one blocked primer that contains a 5′ domain, at least one nucleotide that is cleavable by an RNase H enzyme, a 3′ domain, wherein the primer is not extendable by a polymerase, and wherein the 3′ domain has a length of 7-100 nucleotides, for example 10-30 nucleotides. These methods and the use of a blocked primer reduce or eliminate non-specific amplification products, such as primer dimers, which are generated in RPA reactions.

公开(公告)号：US20190255505A1

公开(公告)日：2019-08-22

申请号：US16403339

申请日：2019-05-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham Rosenbaum ,  Collyn Seeger ,  Jeremy Gray ,  Hua Yu

IPC: B01J19/00 ,  C12Q1/6853

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.