公开(公告)号：US20170037458A1

公开(公告)日：2017-02-09

申请号：US15297348

申请日：2016-10-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John BISHOP ,  Jun LEE

IPC: C12Q1/68 ,  C12N9/00 ,  C07K16/40 ,  C12P19/34

CPC classification number: C07K16/40 ,  C07K2317/24 ,  C07K2317/76 ,  C12N9/93 ,  C12P19/34 ,  C12Q1/6848 ,  C12Q2527/127 ,  C12Q2521/119 ,  C12Q2521/101

Abstract: The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity. The disclosed multi-component inhibitors are useful for DNA sequencing, nucleic acid amplification, cloning and synthesis, and the like.

Abstract translation: 本发明提供核酸聚合酶的多组分抑制剂，制备方法及其使用方法。 多组分抑制剂的一个组分是结合聚合酶（即，聚合酶结合分子（PBM））的分子，但不会基本上抑制其聚合酶活性。 另一个组分是结合PBM（即PBM结合分子）的分子或分子复合物。 PBM和PBM结合分子/复合物的组合基本上抑制聚合酶活性。 所公开的多组分抑制剂可用于DNA测序，核酸扩增，克隆和合成等。