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    MULTI-COMPONENT INHIBITORS OF NUCLEIC ACID POLYMERASES
    1.
    发明申请
    MULTI-COMPONENT INHIBITORS OF NUCLEIC ACID POLYMERASES 审中-公开
    核酸聚合物的多组分抑制剂

    公开(公告)号：US20170037458A1

    公开(公告)日：2017-02-09

    申请号：US15297348

    申请日：2016-10-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John BISHOP ,  Jun LEE

    IPC: C12Q1/68 ,  C12N9/00 ,  C07K16/40 ,  C12P19/34

    CPC classification number: C07K16/40 ,  C07K2317/24 ,  C07K2317/76 ,  C12N9/93 ,  C12P19/34 ,  C12Q1/6848 ,  C12Q2527/127 ,  C12Q2521/119 ,  C12Q2521/101

    Abstract: The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity. The disclosed multi-component inhibitors are useful for DNA sequencing, nucleic acid amplification, cloning and synthesis, and the like.

    Abstract translation: 本发明提供核酸聚合酶的多组分抑制剂，制备方法及其使用方法。 多组分抑制剂的一个组分是结合聚合酶（即，聚合酶结合分子（PBM））的分子，但不会基本上抑制其聚合酶活性。 另一个组分是结合PBM（即PBM结合分子）的分子或分子复合物。 PBM和PBM结合分子/复合物的组合基本上抑制聚合酶活性。 所公开的多组分抑制剂可用于DNA测序，核酸扩增，克隆和合成等。

    COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR)
    2.
    发明申请
    COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR) 有权

    公开(公告)号：US20130164788A1

    公开(公告)日：2013-06-27

    申请号：US13764754

    申请日：2013-02-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jun LEE ,  Ayoub Rashtchian

    IPC: C12P19/34

    CPC classification number: C12P19/34 ,  C12N9/1276 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/149 ,  C12Q2527/125 ,  C12Q2521/107

    Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

    Methods of Synthesizing and Labeling Nucleic Acid Molecules
    4.
    发明申请
    Methods of Synthesizing and Labeling Nucleic Acid Molecules 审中-公开
    合成和标记核酸分子的方法

    公开(公告)号：US20150126723A1

    公开(公告)日：2015-05-07

    申请号：US14533029

    申请日：2014-11-04

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jun LEE ,  Weidong ZHENG

    IPC: C07H21/04 ,  C07H1/00

    CPC classification number: C07H21/04 ,  C07H1/00 ,  C07H21/02 ,  C12Q1/6813

    Abstract: The present invention is generally related to composition, kits and methods for synthesizing nucleic acid molecules and particularly for synthesizing labeled nucleic acid molecules. Specifically, the invention relates to methods, kits and compositions for synthesizing indirectly labeled nucleic acid molecules. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection, diagnostics, and array analysis.

    Abstract translation: 本发明通常涉及用于合成核酸分子，特别是用于合成标记的核酸分子的组合物，试剂盒和方法。 具体地，本发明涉及用于合成间接标记的核酸分子的方法，试剂盒和组合物。 根据本发明生产的标记的核酸分子特别适用于用于核酸检测，诊断和阵列分析的标记探针。

    SEPARATION AND PURIFICATION OF NUCLEIC ACID FROM PARAFFIN-CONTAINING SAMPLES
    5.
    发明申请
    SEPARATION AND PURIFICATION OF NUCLEIC ACID FROM PARAFFIN-CONTAINING SAMPLES 审中-公开
    从含PARAFFIN的样品中分离和纯化核酸

    公开(公告)号：US20160215281A1

    公开(公告)日：2016-07-28

    申请号：US15003179

    申请日：2016-01-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Song-Hua KE ,  Craig BRANCH ,  Jun LEE ,  Todd Peterson ,  Karl HECKER

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1003 ,  C07H1/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/101

    Abstract: Disclosed are rapid and reproducible methods for separating and purifying nucleic acids from paraffin-containing tissue samples. The disclosed methods involve a melting step, where paraffin-containing tissue samples are heated in the presence of a detergent, causing the paraffin to melt and tissue sample cells to lyse. From the resulting two-phase mixture (i.e., a paraffin phase and an aqueous phase), the aqueous phase is collected for purification of nucleic acid. Protease(s) can be used at one or more points in the separation process to facilitate tissue cell lysis and/or degrade proteins that could degrade nucleic acid or interfere with subsequent genetic manipulation or analysis.

    Abstract translation: 公开了用于从含石蜡组织样品中分离和纯化核酸的快速和可重复的方法。 所公开的方法包括熔融步骤，其中在洗涤剂存在下加热含石蜡的组织样品，使石蜡熔化并组织样品细胞裂解。 从所得的两相混合物（即，石蜡相和水相）中收集水相以纯化核酸。 可以在分离过程中的一个或多个点使用蛋白酶，以促进组织细胞裂解和/或降解可能降解核酸或干扰后续遗传操作或分析的蛋白质。

    COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR)
    6.
    发明申请
    COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR) 有权
    反向转录酶 - 聚合酶链反应的组合物和方法（RT-PCR）

    公开(公告)号：US20150017688A1

    公开(公告)日：2015-01-15

    申请号：US14498827

    申请日：2014-09-26

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jun LEE ,  Ayoub RASHTCHIAN

    IPC: C12P19/34 ,  C12N9/12

    CPC classification number: C12P19/34 ,  C12N9/1276 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/149 ,  C12Q2527/125 ,  C12Q2521/107

    Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

    Abstract translation: 本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。 具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法 （或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。 因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。 本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

    METHODS OF SYNTHESIZING AND LABELING NUCLEIC ACID MOLECULES
    8.
    发明申请
    METHODS OF SYNTHESIZING AND LABELING NUCLEIC ACID MOLECULES 审中-公开

    公开(公告)号：US20180371008A1

    公开(公告)日：2018-12-27

    申请号：US16023051

    申请日：2018-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jun LEE ,  Weidong ZHENG

    IPC: C07H21/04 ,  C07H21/02 ,  C12Q1/6813 ,  C07H1/00

    Abstract: The present invention is generally related to composition, kits and methods for synthesizing nucleic acid molecules and particularly for synthesizing labeled nucleic acid molecules. Specifically, the invention relates to methods, kits and compositions for synthesizing indirectly labeled nucleic acid molecules. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection, diagnostics, and array analysis.

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