公开(公告)号：US20230131285A1

公开(公告)日：2023-04-27

申请号：US17889654

申请日：2022-08-17

Applicant: Life Technologies Corporation

Inventor： Shrutii SARDA ,  Geoffrey LOWMAN

IPC: C12Q1/6883

Abstract: Prediction of a clinical response to a therapy of a subject with an autoimmune disease based on B cell immune repertoire may include determining a plurality of clone frequencies in a biological sample from the subject, wherein the clone frequencies include: IgM, IgD, IgG3, IgG4, IgA, IgM with first somatic hypermutation (SHM) level, IgG1 with second SHM level. A plurality of decision criteria may be applied to features including the plurality of clone frequencies. Each decision criterion applies at least one threshold to a feature, wherein the plurality of decision criteria provides a plurality of output values. The plurality of output values may be summed and a sigmoid transformation may be applied to the summed value to form a prediction value. The prediction value may be compared to a final threshold to identify the subject as a likely responder or non-responder to an autoimmune disease therapy.

公开(公告)号：US20220251669A1

公开(公告)日：2022-08-11

申请号：US17658493

申请日：2022-04-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shrutii SARDA ,  Anna MCGEACHY ,  Rajesh GOTTIMUKKALA ,  David MERRILL ,  Heesun SHIN ,  Aren EWING ,  Wing LEE ,  Birgit DREWS ,  Fiona HYLAND ,  Janice AU-YOUNG

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/686 ,  G16B30/10

Abstract: The present disclosure provides compositions and methods, as well as combinations, kits, and systems that include the compositions and methods, for amplification, detection, characterization, assessment, profiling and/or measurement of nucleic acids in samples, particularly biological samples. Compositions and methods provided herein include combinations of microbial species target-specific nucleic acid primers for selective amplification and/or combinations of primers for amplification of nucleic acids from a large group of taxonomically related microorganisms. In one aspect, amplified nucleic acids obtained using the compositions and methods can be used in various processes including nucleic acid sequencing and used to detect the presence of microbial species and assess microbial populations in a variety of samples. In accordance with the teachings and principles, new methods, systems and non-transitory machine-readable storage medium are provided to compress reference sequence databases used in mapping sequence reads for analysis and profiling of microbial populations.