公开(公告)号：US06229024B1

公开(公告)日：2001-05-08

申请号：US09234766

申请日：1999-01-21

申请人： Laurence C. Schmued

发明人： Laurence C. Schmued

IPC分类号： C07D31180

CPC分类号： C07D311/80 ,  C09B11/08 ,  G01N1/30 ,  G01N33/6896 ,  G01N2001/302

摘要： An anionic fluorescein homologue capable of selectively staining degenerating neurons in brain slices, the method of making the anionic fluorescein homologue, and the method of using the same for detecting neuronal degeneration. Two anionic fluorescein based fluorescent stains are the water soluble tribasic salt of a mixture of the fluorescein isomers 5-carboxyfluorescein and 6-carboxyfluorescein, and the water soluble tetrabasic salt of 5,6 dicarboxyfluorescein.

摘要翻译： 能够选择性地染色脑切片中的退化神经元的阴离子荧光素同系物，制备阴离子荧光素同源物的方法，以及使用该阴离子荧光素同源物检测神经元变性的方法。 两种基于阴离子荧光素的荧光染料是荧光素异构体5-羧基荧光素和6-羧基荧光素的混合物的水溶性三元盐，以及5,6二羧基荧光素的水溶性四元盐。

公开(公告)号：US5078041A

公开(公告)日：1992-01-07

申请号：US532594

申请日：1990-06-04

申请人： Laurence C. Schmued

发明人： Laurence C. Schmued

IPC分类号： G10H3/18

CPC分类号： G10H3/185 ,  G10H2220/471 ,  G10H2220/501 ,  G10H2220/555 ,  Y10S84/24

摘要： A suspension bridge pickup mechanism for stringed musical instruments. Vibrations from the strings are transferred to a suspended bridge which is in contact at spaced points with resonator plates of a plurality of bimorphic piezoelectric elements. The bridge has a high length to transverse thickness ratio resulting in a signal of wide frequency range and high amplitude. The tonal characteristics and the relative harmonic content of the signal can be altered by selecting or rejecting specific transducers which will contribute to the output signal utilizing a switching circuit. The transducers may be positioned under different portions of the bridge resulting in greater low frequency response in the median region of the bridge, maximal mid frequency response in the paramedian region, and maximal high frequency response near the ends of the bridge. Resonator plates of large area and small thickness are employed for maximal reproduction of low frequencies and resonator plates of small area and greater thickness are employed for maximal reproduction of high frequencies. The instrument strings cross and are supported by the bridge asymmetrically of its length.

摘要翻译： 用于弦乐器的悬吊桥拾取机构。 来自琴弦的振动被转移到与多个双压电元件的谐振器板在间隔点处接触的悬挂桥。 该桥具有高长度与横向厚度比，导致宽频率范围和高振幅的信号。 可以通过选择或拒绝使用切换电路有助于输出信号的特定换能器来改变信号的音调特性和相对谐波含量。 换能器可以位于桥的不同部分下，导致桥的中间区域中的较低的低频响应，辅助区域中的最大中频响应以及桥端部附近的最大高频响应。 大面积和小厚度的谐振器板用于低频的最大再现，并且使用小面积和较大厚度的谐振器板用于高频率的最大再现。 仪器串交叉并由桥梁不对称地支撑其长度。

公开(公告)号：US4716905A

公开(公告)日：1988-01-05

申请号：US780100

申请日：1985-09-25

申请人： Laurence C. Schmued

发明人： Laurence C. Schmued

IPC分类号： A61K49/00 ,  A61B5/05

CPC分类号： A61K49/0021

摘要： A fluorescent retrograde axonal tracer method for use in neuroanatomical studies of the brain involves the use of dilute solutions of amidino-stilbene compounds or salts, specifically 2-hydroxy-4,4'-diamidinostilbene isethionate. The amidino stilbene tracer exhibits a high sensitivity and fluorescent intensity, retrogradely fills axons and dendrites extensively, is highly resistant to fading, is compatible with virtually all other histochemical procedures, and exhibits minimal uptake by fibers of passage. The method of using amidino stilbene or stilbamadine compounds involves injection of a dilute solution of the compound into the area of the brain to be studied. After a period of time, the tissue is examined under a fluorescence microscope.

摘要翻译： 用于脑的神经解剖学研究的荧光逆行轴突示踪方法涉及使用脒基 - 二苯乙烯化合物或盐，特别是2-羟基-4,4'-二脒基苯乙烯磺酸盐的稀释溶液。 脒基二苯乙烯示踪剂具有高灵敏度和荧光强度，广泛地逆行填充轴突和树突，具有高度抗褪色性，几乎与所有其他组织化学方法相容，并且表现出通过纤维的最小吸收。 使用脒基二苯乙烯或芪巴胺化合物的方法包括将化合物的稀释溶液注入待研究的脑区域。 一段时间后，在荧光显微镜下检查组织。

公开(公告)号：US06372451B1

公开(公告)日：2002-04-16

申请号：US09840628

申请日：2001-04-23

申请人： Laurence C. Schmued

发明人： Laurence C. Schmued

IPC分类号： G01N130

CPC分类号： G01N1/30 ,  C01B25/45 ,  G01N2001/302

摘要： A novel aurophosphate stain for staining a slide-mounted brain tissue slice or section to label myelin therein, the method of staining, and the method of making the stain. The stain is potassium aurophosphate or sodium aurophosphate produced as the reaction product of an aurochloride and a dibasic potassium or sodium phosphate. Slide-mounted brain tissue slices are stained by immersing the slice in a warn solution of the aurophosphate. The stained slice may be intensified by immersing the slide-mounted stained tissue slice in a potassium tetrachloroaureate solution. The stained or intensified slice can be fixed by immersion in a sodium thiosulfate solution. Large bundles of stained myelin appear deep red-brown, while smaller bundles and individual fibers appear black.

摘要翻译： 一种用于染色载玻片安装的脑组织切片或切片以标记髓磷脂的新型磷酸盐染色剂，染色方法以及染色方法。 该染色剂是由磷酸二氢钾或磷酸氢二钠作为反应产物产生的磷酸二钾或焦磷酸钠。 通过将切片浸入磷酸氢盐的警告溶液中来将滑动安装的脑组织切片染色。 通过将载玻片安装的染色的组织切片浸入四氯化碳酸钾溶液中可以增强染色的切片。 通过浸入硫代硫酸钠溶液中可以固定染色或加强的切片。 染色髓鞘的大束出现深红棕色，较小的束和单个纤维呈黑色。