公开(公告)号：US06743601B1

公开(公告)日：2004-06-01

申请号：US09456042

申请日：1999-12-06

申请人： Robert F. Bonner ,  Seth R Goldstein ,  Paul D. Smith ,  Thomas Pohida

发明人： Robert F. Bonner ,  Seth R Goldstein ,  Paul D. Smith ,  Thomas Pohida

IPC分类号： G01N130

CPC分类号： G01N1/2813 ,  G01N2001/284 ,  Y10T428/2813 ,  Y10T428/2839 ,  Y10T436/25375

摘要： An apparatus and process for the micro juxtaposition is set forth where a selectively activatable surface is maintained spaced apart from the tissue sample and juxtaposed to the tissue sample by activation. In the typical case, activation occurs by laser radiation with the material of the activatable surface thermally expanding and bringing about the desired micro juxtaposition. The disclosed micro juxtapositioning can cause locally and microscopically pressure on tissue sample, insertion to the tissue sample, or contact of an activated or prepared surface to the tissue sample.

摘要翻译： 阐述了用于微并列的装置和方法，其中可选择性激活的表面保持与组织样本间隔开并且通过激活并置到组织样本。 在典型情况下，通过激活辐射发生激活，其中可激活表面的材料热膨胀并产生所需的微并置。 所公开的微并置可能导致组织样本的局部和显微压力，组织样本的插入或活化或制备的表面与组织样品的接触。

公开(公告)号：US06465208B1

公开(公告)日：2002-10-15

申请号：US09806267

申请日：2001-07-06

申请人： Rick A. Rogers

发明人： Rick A. Rogers

IPC分类号： G01N130

CPC分类号： G01N1/30 ,  G01N2001/302 ,  Y10S435/968

摘要： The present invention clarifies and contrasts stain intact biological tissue samples for microscopic analysis. A biological specimen is depigmented and then contacted with a fluorescent dye, wherein the dye stains the specimen nonspecifically. The depigmented, non-specifically stained specimen is equilibrated in a clearing solution, wherein the refractive index of the clearing solution approximates that of the specimen, thereby preparing the biological specimen for visual analysis.

摘要翻译： 本发明澄清和对比染色完整的生物组织样品用于显微镜分析。 生物样品脱色，然后与荧光染料接触，其中染料非特异性地染色样品。 脱色的非特异染色的样品在清除溶液中平衡，其中清除溶液的折射率近似于样品的折射率，从而制备用于目视分析的生物样品。

公开(公告)号：US06531317B2

公开(公告)日：2003-03-11

申请号：US09741861

申请日：2000-12-22

申请人： Raouf A. Guirguis ,  Mariamena El-Amin

发明人： Raouf A. Guirguis ,  Mariamena El-Amin

IPC分类号： G01N130

CPC分类号： G01N1/30 ,  Y10T436/10 ,  Y10T436/108331

摘要： The present invention is directed to a fixative composition, the use of the fixative composition in preparing cytological or histological specimens, and a method of preparing particulate matter, such as cytology, hematology and microbiology specimens, for examination by collecting the particulate matter in a uniform layer, preferably a monolayer, and fixing the particles in a composition according to the present invention. The cytological, hematological, microbiological and histological fixative composition of the present invention contains an aldehyde crosslinker, a polyol and a detergent. The method of the present invention for preserving the particulate or histological specimen uses the fixative composition containing an aldehyde crosslinker, a polyol and a detergent.

公开(公告)号：US06436663B1

公开(公告)日：2002-08-20

申请号：US09709437

申请日：2000-11-13

申请人： Mark H. Potts

发明人： Mark H. Potts

IPC分类号： G01N130

CPC分类号： G01N1/30

摘要： A method of simultaneously staining for various opportunistic pathogens stains tissues sections with Ziehl-Neelsen carbo-fuchsin, Schiff reagent, and a mixture of metanil yellow and stock fast green solution. In particular, the method is useful for staining tissue sections suspected of containing opportunistic pathogens commonly found in AIDS patients, i.e., acid fast mycobacteria, fungus, and Pneumocystis carinii.

摘要翻译： 一种同时染色各种机会性病原体的染色方法，用Ziehl-Neelsen碳 - 品红，席夫试剂以及淡黄色和原料快绿色溶液的混合物染色组织切片。 特别地，该方法可用于染色怀疑含有艾滋病患者通常发现的机会性病原体的组织切片，即耐酸分枝杆菌，真菌和卡氏肺囊虫。

公开(公告)号：US06387653B1

公开(公告)日：2002-05-14

申请号：US09289181

申请日：1999-04-09

申请人： John Voneiff ,  Jay Frederick Gibson

发明人： John Voneiff ,  Jay Frederick Gibson

IPC分类号： G01N130

CPC分类号： G01N1/312 ,  G01N1/06 ,  Y10T83/472 ,  Y10T83/6492 ,  Y10T83/9292

摘要： An apparatus and method for producing tissue slides is disclosed. The apparatus includes a holding assembly for manipulating the sample block, a blade assembly for preparing a thin section from the sample block, and a transfer roller mechanism for transferring the thin section to a receiving medium. The apparatus further includes a controller that may track the sample block and thin section. The method includes the steps of first locating a sample embedded within a support medium, which may be paraffin or a similar medium. Next, the embedded sample is oriented in such a way that its working surface is presented. This orientation may entail determining the orientation of the embedded sample with respect to the blade that will produce the largest cross-sectional area. Next, a slice of the sample from said embedded sample is removed and subsequently transferred to a suitable receiving medium, which may include a microscope slide.

摘要翻译： 公开了一种用于生产组织载玻片的装置和方法。 该装置包括用于操作样品块的保持组件，用于从样品块制备薄部分的刀片组件和用于将薄部分转移到接收介质的转印辊机构。 该装置还包括可以跟踪样品块和薄部分的控制器。 该方法包括以下步骤：首先将嵌入在载体介质（其可以是石蜡或类似介质）中​​的样品定位。 接下来，嵌入式样品被定向成使其工作表面呈现。 该取向可能需要确定嵌入样品相对于将产生最大横截面积的叶片的取向。 接下来，去除来自所述嵌入样品的样品切片并随后转移到合适的接收介质，其可以包括显微镜载玻片。

公开(公告)号：US06372512B1

公开(公告)日：2002-04-16

申请号：US09154430

申请日：1998-09-16

申请人： Russell L. Kerschmann

发明人： Russell L. Kerschmann

IPC分类号： G01N130

CPC分类号： G01N1/30 ,  G01N1/36 ,  Y10T436/25

摘要： A method for preparing an organic sample for cutting and subsequent examination, involving immersing the sample in a composition containing: an infiltrating substance; an embedding substance, which can be the same or different from the infiltrating substance; and a stain that chemically associates with the organic sample, wherein the stain exhibits different detectable properties when associated and not associated with the sample.

摘要翻译： 一种制备用于切割和随后检查的有机样品的方法，包括将样品浸入含有：渗透物质的组合物中; 与渗透物质相同或不同的包埋物质; 以及与有机样品化学结合的污渍，其中当相关联并且不与样品相关联时，染色剂显示不同的可检测性质。

公开(公告)号：US06372451B1

公开(公告)日：2002-04-16

申请号：US09840628

申请日：2001-04-23

申请人： Laurence C. Schmued

发明人： Laurence C. Schmued

IPC分类号： G01N130

CPC分类号： G01N1/30 ,  C01B25/45 ,  G01N2001/302

摘要： A novel aurophosphate stain for staining a slide-mounted brain tissue slice or section to label myelin therein, the method of staining, and the method of making the stain. The stain is potassium aurophosphate or sodium aurophosphate produced as the reaction product of an aurochloride and a dibasic potassium or sodium phosphate. Slide-mounted brain tissue slices are stained by immersing the slice in a warn solution of the aurophosphate. The stained slice may be intensified by immersing the slide-mounted stained tissue slice in a potassium tetrachloroaureate solution. The stained or intensified slice can be fixed by immersion in a sodium thiosulfate solution. Large bundles of stained myelin appear deep red-brown, while smaller bundles and individual fibers appear black.

摘要翻译： 一种用于染色载玻片安装的脑组织切片或切片以标记髓磷脂的新型磷酸盐染色剂，染色方法以及染色方法。 该染色剂是由磷酸二氢钾或磷酸氢二钠作为反应产物产生的磷酸二钾或焦磷酸钠。 通过将切片浸入磷酸氢盐的警告溶液中来将滑动安装的脑组织切片染色。 通过将载玻片安装的染色的组织切片浸入四氯化碳酸钾溶液中可以增强染色的切片。 通过浸入硫代硫酸钠溶液中可以固定染色或加强的切片。 染色髓鞘的大束出现深红棕色，较小的束和单个纤维呈黑色。

公开(公告)号：US06774361B2

公开(公告)日：2004-08-10

申请号：US10157232

申请日：2002-05-30

申请人： Moungi G. Bawendi ,  Klavs F. Jensen

发明人： Moungi G. Bawendi ,  Klavs F. Jensen

IPC分类号： G01N130

CPC分类号： G06K19/06046 ,  B82Y15/00 ,  G01N33/532 ,  G01N33/533 ,  G01N33/588 ,  Y10S420/903

摘要： A novel encoding system and methods for determining the location and/or identity of a particular item or component of interest is provided. In particular, the present invention utilizes a “barcode” comprising one or more sizes of semiconductor nanocrystals (quantum dots) having characteristic spectral emissions, to either “track” the location of a particular item of interest or to identify a particular item of interest. The semiconductor nanocrystals used in the inventive “barcoding” scheme can be tuned to a desired wavelength to produce a characteristic spectral emission in narrow spectral widths, and with a symmetric, nearly Gaussian line shape, by changing the composition and size of the quantum dot. Additionally, the intensity of the emission at a particular characteristic wavelength can also be varied, thus enabling the use of binary or higher order encoding schemes. The information encoded by the quantum dot can be spectroscopically decoded, thus providing the location and/or identity of the particular item or component of interest. In particular, a single primary light source can be used to decode the inventive barcode. In particularly preferred embodiments, the present system and method is used in applications to security systems, to the tracking of consumer items such as jewelry, vehicles, or paper. In other particularly preferred embodiments, the present system and method is used in applications for biochemistry to track the location of biomolecules such as DNA sequences, combinatorial chemistry, and genomics for encoding and probe identifiers.

摘要翻译： 提供了一种新颖的编码系统和用于确定感兴趣的特定项目或组件的位置和/或身份的方法。 特别地，本发明利用包括具有特征光谱发射的一个或多个尺寸的半导体纳米晶体（量子点）的“条形码”来“跟踪”特定感兴趣的物体的位置或识别特定的感兴趣物品。 在本发明的“条形码”方案中使用的半导体纳米晶体可以通过改变量子点的组成和尺寸而被调谐到期望的波长以产生窄光谱宽度的特征光谱发射，并具有对称的几乎高斯线形状。 此外，特定特征波长处的发射强度也可以变化，从而能够使用二进制或更高阶编码方案。 由量子点编码的信息可以进行光谱解码，从而提供感兴趣的特定项目或组件的位置和/或身份。 特别地，可以使用单个主光源来解码本发明的条形码。 在特别优选的实施例中，本系统和方法用于安全系统的应用，对诸如珠宝，车辆或纸张的消费品的跟踪。 在其它特别优选的实施方案中，本系统和方法用于生物化学应用以跟踪生物分子的位置，例如编码和探针标识符的DNA序列，组合化学和基因组学。

公开(公告)号：US06746851B1

公开(公告)日：2004-06-08

申请号：US09483248

申请日：2000-01-14

申请人： Ken K. Tseung ,  Glenn Takayama ,  Norman K. Rhett ,  Mark V. Corl

发明人： Ken K. Tseung ,  Glenn Takayama ,  Norman K. Rhett ,  Mark V. Corl

IPC分类号： G01N130

CPC分类号： G01N1/312

摘要： A method and apparatus for specimen slide (710) preparation is disclosed. The method and apparatus of the present invention uses slide trays (700) that have receptacles for at least one specimen slide (710) and an associated reagent pack (720). The specimen slide (710) and reagent pack (720) includes respective identifiers (420, 411) that specify a particular slide preparation protocol that should be followed. The method reads the identifiers (420, 411) on the reagent pack and the slide to verify that the correct slide preparation protocol is being used.

摘要翻译： 公开了一种用于标本滑动（710）制备的方法和装置。 本发明的方法和装置使用具有用于至少一个标本滑块（710）和相关联的试剂盒（720）的插座的滑动托盘（700）。 标本滑块（710）和试剂盒（720）包括指定应遵循的特定幻灯片准备协议的相应标识符（420,411）。 该方法读取试剂盒和幻灯片上的标识符（420,411），以验证是否正在使用正确的幻灯片准备协议。

公开(公告)号：US06709836B2

公开(公告)日：2004-03-23

申请号：US09968043

申请日：2001-10-02

申请人： Wilhelmus Hermanus Hendrikus Maria Kieftenbeld

发明人： Wilhelmus Hermanus Hendrikus Maria Kieftenbeld

IPC分类号： G01N130

CPC分类号： C08J3/092 ,  C08J2333/06 ,  G01N1/30 ,  G02B21/34 ,  Y10T428/2891

摘要： The invention relates to a mounting medium that can be used in the preparation of slides for investigation by microscopy techniques. More in particular the mounting medium comprises a solution, in at least one organic solvent selected from the group consisting of saturated hydrocarbons optionally mixed with one or more alcohols, of at least one (meth)acrylate resin based on one or more monomers having formula I: wherein R is hydrogen or a methyl group and n has a value of 0-19. The mounting medium of the invention is particularly suited for use in histochemistry, immunochemistry and/or cytochemistry, i.e. to provide slides of samples of blood, cells tissue or other biological fluids or materials, including but not limited to samples/materials which have been stained to facilitate microscopic examination, e.g. for scientific and/or diagnostic purposes.

摘要翻译： 本发明涉及一种可用于制备幻灯片以用显微技术研究的安装介质。 更具体地说，安装介质包含在至少一种选自基于一种或多种具有式I的单体的至少一种（甲基）丙烯酸酯树脂的任选与一种或多种醇混合的饱和烃的有机溶剂中的溶液 ：其中R是氢或甲基，n的值为0-19.本发明的安装介质特别适用于组织化学，免疫化学和/或细胞化学，即提供血样，细胞组织 或其他生物流体或材料，包括但不限于已经被染色以促进显微镜检查的样品/材料，例如， 用于科学和/或诊断目的。