公开(公告)号：US20240094110A1

公开(公告)日：2024-03-21

申请号：US18240441

申请日：2023-08-31

Applicant: Life Technologies Corporation

Inventor： Michael D Ward ,  Gregory Kaduchak ,  Dmitriy Strelkov

IPC: G01N15/14

CPC classification number: G01N15/1436 ,  G01N2015/0065

Abstract: Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

公开(公告)号：US11788949B2

公开(公告)日：2023-10-17

申请号：US17548964

申请日：2021-12-13

Applicant: Life Technologies Corporation

Inventor： Michael D Ward ,  Gregory Kaduchak ,  Dmitriy Strelkov

IPC: G01N15/14 ,  G01N15/00

CPC classification number: G01N15/1436 ,  G01N2015/0065 ,  G01N2015/1438 ,  G01N2015/1443

Abstract: Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

公开(公告)号：US20220187189A1

公开(公告)日：2022-06-16

申请号：US17548964

申请日：2021-12-13

Applicant: Life Technologies Corporation

Inventor： Michael D Ward ,  Gregory Kaduchak ,  Dmitriy Strelkov

IPC: G01N15/14

Abstract: Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.