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    Systems and methods for improved imaging and fluorescence in flow cytometry and other applications
    2.
    发明授权
    Systems and methods for improved imaging and fluorescence in flow cytometry and other applications 有权

    公开(公告)号：US11788949B2

    公开(公告)日：2023-10-17

    申请号：US17548964

    申请日：2021-12-13

    Applicant: Life Technologies Corporation

    Inventor： Michael D Ward ,  Gregory Kaduchak ,  Dmitriy Strelkov

    IPC: G01N15/14 ,  G01N15/00

    CPC classification number: G01N15/1436 ,  G01N2015/0065 ,  G01N2015/1438 ,  G01N2015/1443

    Abstract: Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

    Systems And Methods For Improved Imaging And Fluorescence In Flow Cytometry And Other Applications
    3.
    发明公开
    Systems And Methods For Improved Imaging And Fluorescence In Flow Cytometry And Other Applications 审中-公开

    公开(公告)号：US20240094110A1

    公开(公告)日：2024-03-21

    申请号：US18240441

    申请日：2023-08-31

    Applicant: Life Technologies Corporation

    Inventor： Michael D Ward ,  Gregory Kaduchak ,  Dmitriy Strelkov

    IPC: G01N15/14

    CPC classification number: G01N15/1436 ,  G01N2015/0065

    Abstract: Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

    Reduced Speckle Illumination Systems And Methods
    4.
    发明申请
    Reduced Speckle Illumination Systems And Methods 有权

    公开(公告)号：US20220187614A1

    公开(公告)日：2022-06-16

    申请号：US17549114

    申请日：2021-12-13

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LTD

    Inventor： Michael D Ward ,  Gregory Kaduchak ,  Lingzhi ZHU

    IPC: G02B27/48 ,  F21V8/00 ,  G02B6/44 ,  G02B6/028

    Abstract: Provided are laser sources, the laser sources comprising at least one diode; and an optic fiber of a predefined length disposed between the laser source and a position for a target such that the optic fiber communicates light pulses from the laser source as a source light to the position for the target, wherein the position is illuminated by the source light so as to reduce speckles in a captured image of the target. Also provided are methods for providing source light for generating an image, comprising: generating illumination with one or more laser diodes; and passing the illumination through an optic fiber having a plurality of bends therein such that source light is emitted from the optic fiber so as to illuminate a target with the source light, the source light reducing speckles in an image of the target.

    Systems And Methods For Improved Imaging And Fluorescence In Flow Cytometry And Other Applications
    5.
    发明申请
    Systems And Methods For Improved Imaging And Fluorescence In Flow Cytometry And Other Applications 有权

    公开(公告)号：US20220187189A1

    公开(公告)日：2022-06-16

    申请号：US17548964

    申请日：2021-12-13

    Applicant: Life Technologies Corporation

    Inventor： Michael D Ward ,  Gregory Kaduchak ,  Dmitriy Strelkov

    IPC: G01N15/14

    Abstract: Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

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