公开(公告)号：US20200047093A1

公开(公告)日：2020-02-13

申请号：US16341326

申请日：2017-10-18

Applicant: Massachusetts Institute of Technology

Inventor： Michael S. Bono ,  Sydney B. Beasley ,  Emily Barret Hanhauser ,  Chintan Vaishnav ,  Anastasios John Hart ,  Rohit Nandkumar Karnik

IPC: B01D35/06 ,  B01L3/00 ,  G01N1/10

Abstract: Systems, devices, and methods for detecting contamination (e.g., bacteria) in fluid are provided. The systems, devices, and methods allow for filtering a fluid sample using a filter to capture and concentrate cells (e.g., bacteria) to detect electrochemical properties thereof. The cells can be exposed to a reagent that diffuses into the cells to produce a product of interest that can be used in analysis of the fluid sample. The product of interest can diffuse out of the filter into a fluid storage component for detection and analysis by an analysis component. After the sampling is completed, the filter can be detached and discarded. Other aspects of the present disclosure, including enhancements and various systems and methods for concentrating cells and analyzing the same, are also provided.

公开(公告)号：US11612839B2

公开(公告)日：2023-03-28

申请号：US16341326

申请日：2017-10-18

Applicant: Massachusetts Institute of Technology

Inventor： Michael S. Bono ,  Sydney B. Beasley ,  Emily Barret Hanhauser ,  Chintan Vaishnav ,  Anastasios John Hart ,  Rohit Nandkumar Karnik

IPC: G01N1/10 ,  B01D35/06 ,  B01L3/00

Abstract: Systems, devices, and methods for detecting contamination (e.g., bacteria) in fluid are provided. The systems, devices, and methods allow for filtering a fluid sample using a filter to capture and concentrate cells (e.g., bacteria) to detect electrochemical properties thereof. The cells can be exposed to a reagent that diffuses into the cells to produce a product of interest that can be used in analysis of the fluid sample. The product of interest can diffuse out of the filter into a fluid storage component for detection and analysis by an analysis component. After the sampling is completed, the filter can be detached and discarded. Other aspects of the present disclosure, including enhancements and various systems and methods for concentrating cells and analyzing the same, are also provided.