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公开(公告)号：US20210239664A1
公开(公告)日：2021-08-05
申请号：US17263080
申请日：2019-07-31
申请人： Mie University
摘要： The present invention aims to provide a method for preparing exosomes comprising: (i) a step for ultrafiltering a sample containing at least one exosome; and (ii) a step for subjecting the sample that can be obtained from step (i) to anion exchange column chromatography.
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公开(公告)号：US11714068B2
公开(公告)日：2023-08-01
申请号：US17263080
申请日：2019-07-31
申请人： Mie University
CPC分类号： G01N30/88 , G01N1/405 , G01N1/4077 , G01N2001/4088
摘要： The disclosure provides a method for preparing exosomes using: (i) a step for ultrafiltering a sample containing at least one exosome; and (ii) a step for subjecting the sample that can be obtained from step (i) to anion exchange column chromatography.
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公开(公告)号：US11083744B2
公开(公告)日：2021-08-10
申请号：US15559771
申请日：2016-03-18
申请人： MIE UNIVERSITY , KYOTO UNIVERSITY
IPC分类号： A61K31/7105 , A61P35/00 , A61K35/17 , C12Q1/6876 , C12N5/0775 , A61K9/08 , A61K9/00 , A61K45/06
摘要： Therapeutic agents effective for treating cell-proliferative diseases contain extracellular vesicles (exosomes) released from cytotoxic T cells or miRNA obtained from extracellular vesicles (exosomes) released from cytotoxic T cells, such as human CD8+ T cells. Such therapeutic agents suppress the proliferation of mesenchymal cells surrounding cancer cells, e.g., by killing the mesenchymal cells, such that the cancer cells become isolated and unable to metastasize. Cell-proliferative diseases are thus treatable by administering such a therapeutic agent to a patient.
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