公开(公告)号：US20120237518A1

公开(公告)日：2012-09-20

申请号：US13157551

申请日：2011-06-10

申请人： Miki YAMAGUCHI ,  Kazunori Kato ,  Hirofumi HAMADA ,  Kazuyasu NAKAMURA ,  Yoshiyuki SUGIMOTO ,  Tsuguo KUBOTA ,  Masahiro IKEDA

发明人： Miki YAMAGUCHI ,  Kazunori Kato ,  Hirofumi HAMADA ,  Kazuyasu NAKAMURA ,  Yoshiyuki SUGIMOTO ,  Tsuguo KUBOTA ,  Masahiro IKEDA

IPC分类号： A61K39/395 ,  C12N1/21 ,  C12N5/10 ,  A61P35/00 ,  C07K16/30 ,  G01N33/574 ,  C12N15/13 ,  C12N15/63 ,  C12P21/02

CPC分类号： C07K16/30 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/732 ,  C07K2317/92

摘要： The present invention relates to a monoclonal antibody or an antibody fragment thereof, which binds to the extracellular region of human Trop-2 with high affinity and exhibits high ADCC activity and high antitumor activity; a hybridoma which produces the antibody; a DNA which encodes the antibody; a vector which comprises the DNA; a transformant obtainable by introducing the vector; a process for producing an antibody or an antibody fragment thereof using the hybridoma or the transformant; and a therapeutic agent or a diagnostic agent using the antibody fragment thereof.

摘要翻译： 本发明涉及一种单克隆抗体或其抗体片段，其以高亲和力结合人Trop-2的细胞外区域并表现出高ADCC活性和高抗肿瘤活性; 产生抗体的杂交瘤; 编码抗体的DNA; 包含DNA的载体; 通过引入载体可获得的转化体; 使用杂交瘤或转化体生产抗体或其抗体片段的方法; 以及使用其抗体片段的治疗剂或诊断剂。

公开(公告)号：US20090280564A1

公开(公告)日：2009-11-12

申请号：US12437639

申请日：2009-05-08

申请人： Osamu HONMOU ,  Hirofumi HAMADA

发明人： Osamu HONMOU ,  Hirofumi HAMADA

IPC分类号： C12N5/02

CPC分类号： C12N5/0618 ,  A61K35/12 ,  C12N2500/44 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2506/02 ,  C12N2506/1353 ,  C12N2506/1369

摘要： Mesodermal stem cells or ES cells, prepared from the mononuclear cell fraction isolated from bone marrow fluid or umbilical blood, were found to differentiate into neural stem cells, neurons, or glial cells when cultured in a basal culture medium. In addition, the differentiation of the mesodermal stem cells or ES cells into neural cells was promoted through the addition of an ischemic brain extract to the above-mentioned basal culture medium. Furthermore, the neural cells obtained using the above-described method for inducing differentiation were revealed to have neural regeneration potency in a brain infarction model, a dementia model, a spinal cord injury model and a demyelination model.In addition, according to the present invention, mesodermal stem cells can be differentiated into neural cells by immortalizing the mesodermal stem cells by highly expressing or activating an immortalization gene in the mesodermal stem cells and culturing the cells under an appropriate condition. The methods of the present invention are very useful in the medical field of neural regeneration.

摘要翻译： 当从基础培养基中培养时，发现从分离自骨髓液或脐血的单核细胞级分制备的中胚层干细胞或ES细胞分化为神经干细胞，神经元或神经胶质细胞。 此外，通过向上述基础培养基中加入缺血性脑提取物促进了中胚层干细胞或ES细胞向神经细胞的分化。 此外，使用上述诱导分化方法获得的神经细胞显示在脑梗死模型，痴呆模型，脊髓损伤模型和脱髓鞘模型中具有神经再生效力。 此外，根据本发明，中胚层干细胞可以通过高效表达或激活中胚层干细胞中的永生化基因并在合适的条件下培养细胞，使中胚层干细胞永生化，将其分化为神经细胞。 本发明的方法在神经再生的医学领域中非常有用。