公开(公告)号：US07250095B2

公开(公告)日：2007-07-31

申请号：US10617262

申请日：2003-07-10

申请人： Murdo M. Black ,  Colin Butters ,  Wah O. Ho ,  John Rippeth

发明人： Murdo M. Black ,  Colin Butters ,  Wah O. Ho ,  John Rippeth

IPC分类号： G01N27/327

CPC分类号： C12Q1/005

摘要： A non-mediated enzyme electrode comprises a base substrate (2) on which is provided an electrically conductive base layer (8) comprising finely divided platinum group metal or oxide bonded together by a resin; a top layer on the base layer (8), the top layer comprising a buffer. A catalytically active quantity of an oxidoreductase enzyme is provided in at least one of the base layer and the top layer. The invention also provides a biosensor (20) which includes an enzyme electrode, and methods of manufacturing the enzyme electrode and biosensor.

摘要翻译： 非介导的酶电极包括基底（2），在其上设置有包含细分的铂族金属或通过树脂粘合在一起的氧化物的导电性基底层（8） 在基层（8）上的顶层，顶层包括缓冲液。 催化活性量的氧化还原酶被提供在基层和顶层中的至少一个中。 本发明还提供了包括酶电极的生物传感器（20），以及制造酶电极和生物传感器的方法。

公开(公告)号：US20050150762A1

公开(公告)日：2005-07-14

申请号：US11028941

申请日：2005-01-04

申请人： Colin Butters ,  Wah Ho ,  John Rippeth

发明人： Colin Butters ,  Wah Ho ,  John Rippeth

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001 ,  G01N27/3272

摘要： A biosensor (20) for indicating electrochemically the catalytic activity of an enzyme in the presence of a biological fluid containing an analyte acted upon by said enzyme comprises: (a) a first substrate (2); (b) a second substrate (18) overlying at least a part of the first substrate (2); (c) a working electrode (24) on one of the substrates, the working electrode (24) including a catalytically-active quantity of said enzyme; (d) a counter electrode (22) on one of the substrates; (e) conductive tracks (4, 6) connected to said working (24) and counter (22) electrodes for making electrical connections with a test meter apparatus; (f) a spacer layer (14) having a channel (16) therein and disposed between the first substrate (2) and the second substrate (18), the spacer layer channel (16) co-operating with adjacent surfaces to define a capillary flow path which extends from an edge of at least one of said substrates (2, 18) to said electrodes (22, 24); wherein the electrodes (22, 24) are arranged such that a fluid sample which flows along the capillary flow path from said edge will substantially completely cover the working electrode (24) before the fluid sample makes contact with any part of the counter electrode (22).

摘要翻译： 用于在含有由所述酶作用的分析物的生物流体存在下电化学指示酶的催化活性的生物传感器（20）包括：（a）第一基底（2）; （b）覆盖所述第一基板（2）的至少一部分的第二基板（18）; （c）在一个基板上的工作电极（24），所述工作电极（24）包含催化活性量的所述酶; （d）一个基板上的对置电极（22）; （e）连接到所述工作（24）和计数器（22）电极的导电轨道（4,6），用于与测试仪器设备进行电连接; （f）在其中具有通道（16）并设置在第一基板（2）和第二基板（18）之间的间隔层（14），间隔层通道（16）与相邻表面协作以限定毛细管 从至少一个所述衬底（2,18）的边缘延伸到所述电极（22,24）的流动路径; 其中所述电极（22,24）被布置成使得在所述流体样品与所述对电极（22）的任何部分接触之前，使来自所述边缘的毛细流动路径流动的流体样品将基本上完全覆盖所述工作电极（24） ）。

公开(公告)号：US20050150763A1

公开(公告)日：2005-07-14

申请号：US11028942

申请日：2005-01-04

申请人： Colin Butters ,  Wah Ho ,  Christopher Mayne ,  John Rippeth

发明人： Colin Butters ,  Wah Ho ,  Christopher Mayne ,  John Rippeth

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001 ,  G01N27/3272

摘要： A non-mediated biosensor for indicating amperometrically the catalytic activity of an oxidoreductase enzyme in the presence of a fluid containing a substance acted upon by said enzyme, the biosensor comprising: (a) a first substrate; (b) a working electrode and a counter electrode on the first substrate; (c) conductive tracks connected to said electrodes for making electrical connections with a test meter apparatus; (d) a second substrate overlying at least a part of the first substrate; and (e) a spacer layer having a channel therein and disposed between the first substrate and the second substrate, the spacer layer channel co-operating with adjacent surfaces to define a capillary flow path which does not contain a mesh and which extends from an edge of at least one of said substrates to said electrodes; wherein the working electrode includes: (f) an electrically-conductive base layer comprising particles of finely divided platinum-group metal or platinum-group metal oxide bonded together by a resin; (g) a top layer on the base layer, said top layer comprising a buffer; and (h) a catalytically-active quantity of said oxidoreductase enzyme in at least one of said base layer and said top layer.

摘要翻译： 一种非介导的生物传感器，用于在含有由所述酶作用的物质的流体的存在下，用电子式表示氧化还原酶的催化活性，所述生物传感器包括：（a）第一底物; （b）第一基板上的工作电极和对电极; （c）连接到所述电极的导电轨道，用于与测试仪器设备进行电连接; （d）覆盖所述第一基板的至少一部分的第二基板; 和（e）间隔层，其中具有通道并设置在第一基板和第二基板之间，间隔层通道与相邻表面配合以限定毛细管流动路径，其不包含网状物并且从边缘 至少一个所述衬底到所述电极; 其中所述工作电极包括：（f）导电性基层，其包含通过树脂粘合在一起的细碎的铂族金属或铂族金属氧化物的颗粒; （g）基层上的顶层，所述顶层包括缓冲液; 和（h）在所述基底层和所述顶层中的至少一个中的催化活性量的所述氧化还原酶。

公开(公告)号：US20130112573A1

公开(公告)日：2013-05-09

申请号：US13696380

申请日：2011-05-06

申请人： Michael Noble ,  Craig Nelson ,  Mark Humphries ,  Carys Lloyd ,  David Edington ,  John Rippeth

发明人： Michael Noble ,  Craig Nelson ,  Mark Humphries ,  Carys Lloyd ,  David Edington ,  John Rippeth

IPC分类号： C12Q1/00

CPC分类号： G01N27/3272 ,  C12Q1/006 ,  C12Q1/54 ,  G01N27/3274 ,  G01N33/4915 ,  G01N2496/00

摘要： A method and device are provided for measuring a level of a clinically relevant analyte (such as glucose) in a fluid (such as blood). The device includes a flow path for conducting said fluid through the device; a detection chamber arranged on said flow path; and detector means arranged to detect analyte levels in the fluid in said chamber, wherein: said detection chamber contains a predetermined amount of an analyte such that that analyte mixes with fluid in the detection chamber to form, at the detector means, a calibration sample of the fluid at a time after the arrival of the fluid in said detection chamber, and said detector means is arranged to detect a first analyte level of an unadulterated sample of the fluid at a first time which is before the formation of said calibration sample and to detect a second analyte level of said calibration sample at a second time which is after the formation of said calibration sample.

摘要翻译： 提供了一种用于测量流体（例如血液）中的临床相关分析物（例如葡萄糖）的水平的方法和装置。 该装置包括用于通过该装置传导所述流体的流路; 布置在所述流路上的检测室; 以及检测器装置，其布置成检测所述室中的流体中的分析物水平，其中：所述检测室包含预定量的分析物，使得分析物与检测室中的流体混合，以在检测器装置处形成校准样品 在流体到达所述检测室之后的时间处的流体和所述检测器装置被布置成在形成所述校准样品之前的第一时间检测流体的未掺杂样品的第一分析物水平，并且 在形成所述校准样品之后的第二时间检测所述校准样品的第二分析物水平。

公开(公告)号：US20070017824A1

公开(公告)日：2007-01-25

申请号：US11488326

申请日：2006-07-18

申请人： John Rippeth ,  Wah Ho

发明人： John Rippeth ,  Wah Ho

IPC分类号： G01N33/487 ,  G01F1/64

CPC分类号： G01N27/3272

摘要： A biosensor (12) includes a working electrode (5) and a counter electrode (3), wherein at least a part of the working electrode (5) is disposed between and in electrical connection with two separate conductive tracks (1,2). Other aspects of the invention provide a meter (26) for use with the biosensor (12) and a method of determining whether a fluid sample applied to the biosensor (12) has sufficiently wetted the working electrode (5).

摘要翻译： 生物传感器（12）包括工作电极（5）和对置电极（3），其中工作电极（5）的至少一部分设置在两个独立的导电轨道（1,2）之间并与之连接。 本发明的其它方面提供了一种与生物传感器（12）一起使用的仪表（26），以及一种确定施加到生物传感器（12）上的流体样品是否已充分润湿工作电极（5）的方法。

公开(公告)号：US20070000777A1

公开(公告)日：2007-01-04

申请号：US11445486

申请日：2006-06-01

申请人： Wah Ho ,  John Rippeth ,  Paul Chambers

发明人： Wah Ho ,  John Rippeth ,  Paul Chambers

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001

摘要： A test system for measuring analyte concentration in a fluid sample includes: (i) a capillary-fill biosensor (20) having a working electrode (24), a reference electrode (22) and a separate counter electrode (23), arranged such that a fluid sample which flows evenly along the capillary flow path will substantially completely cover the reference electrode (22) before the fluid sample makes contact with any part of the counter electrode (23); and (ii) a test meter (42) for receiving the biosensor (20), the meter (42) including: first signal circuitry (31) for producing a first signal when an electrical circuit is detected between the reference electrode (22) and the working electrode (24); second signal circuitry (33) for producing a second signal when an electrical circuit is detected between the counter electrode (23) and at least one of the reference electrode (22) and the working electrode (24); a timer (35) for determining the time interval between production of said first signal and said second signal; and a processor (32) for triggering an error condition if said time interval exceeds a preset value or if said second signal is not produced within a preset time after said first signal is produced.

摘要翻译： 用于测量流体样品中的分析物浓度的测试系统包括：（i）具有工作电极（24），参比电极（22）和单独的对置电极（23）的毛细填充生物传感器（20），其布置成 在流体样品与对电极（23）的任何部分接触之前，沿毛细管流动路径均匀流动的流体样品基本上完全覆盖参考电极（22）。 所述仪表（42）包括：第一信号电路（31），用于当在所述参考电极（22）与所述参考电极（22）之间检测到电路时产生第一信号;以及（ii）用于接收所述生物传感器 工作电极（24）; 第二信号电路（33），用于当在对电极（23）和参考电极（22）和工作电极（24）中的至少一个之间检测到电路时产生第二信号; 定时器（35），用于确定所述第一信号的产生与所述第二信号之间的时间间隔; 以及处理器（32），用于如果所述时间间隔超过预设值则触发错误状态，或者如果在产生所述第一信号之后的预设时间内不产生所述第二信号。