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公开(公告)号：US3899376A
公开(公告)日：1975-08-12
申请号：US33498673
申请日：1973-02-23
Applicant: NAT RES DEV
Inventor： RICHMOND WILLIAM
CPC classification number: C12N9/0006 , C12Q1/26 , C12Q1/60 , Y10S210/922 , Y10S435/815 , Y10S435/866 , Y10S435/872 , Y10S435/911 , Y10S435/913 , Y10S435/923 , Y10S435/924 , Y10S435/942
Abstract: Enzyme preparations which will convert cholesterol to Delta 4cholestenone and hydrogen peroxide are obtained from certain Nocardia species belonging to the Mycobacterium rhodocrous group. The preparations have a cholesterol oxidase specific activity of at least 1 unit per 5 mg of protein nitrogen and, when in liquid form, a potency of at least 10 2 units/ml. They are prepared by growing the organism and recovering the enzyme preparation, preferably by extracting the harvested cells with a surface active agent such as Triton X-100. The enzyme preparations are used to assay for cholesterol by measuring the amount in which one of the products of the cholesterol oxidase reaction, preferably hydrogen peroxide, is formed or the quantity in which oxygen is used.
Abstract translation: 将胆固醇转化为DELTA4-胆甾烯酮和过氧化氢的酶制剂从属于分枝杆菌属的诺卡氏菌属获得。 该制剂具有每5mg蛋白质氮至少1单位的胆固醇氧化酶比活性，并且当以液体形式时，其效力为至少10 -3单位/ ml。 它们通过生长生物体并回收酶制剂，优选通过用表面活性剂如Triton X-100萃取收获的细胞来制备。 酶制剂用于通过测量胆固醇氧化酶反应的产物之一，优选过氧化氢的形成量或使用氧气的量来测定胆固醇。
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公开(公告)号：US3907645A
公开(公告)日：1975-09-23
申请号：US43589274
申请日：1974-01-23
Applicant: NAT RES DEV
Inventor： RICHMOND WILLIAM
CPC classification number: C12N9/0006 , C12Q1/26 , C12Q1/60 , Y10S210/922 , Y10S435/815 , Y10S435/866 , Y10S435/872 , Y10S435/911 , Y10S435/913 , Y10S435/923 , Y10S435/924 , Y10S435/942
Abstract: Enzyme preparations which will convert cholesterol to Delta 4cholestenone and hydrogen peroxide are obtained from certain Nocardia species belonging to the Mycobacterium rhodocrous group. The preparations have a cholesterol oxidase specific activity of at least 1 unit per 5 mg of protein nitrogen and, when in liquid form, a potency of at least 10 2 units/ml. They are prepared by growing the organism and recovering the enzyme preparation, preferably by extracting the harvested cells with a surface active agent such as Triton X-100. The enzyme preparations are used to assay for cholesterol by measuring the amount in which one of the products of the cholesterol oxidase reaction, preferably hydrogen peroxide, is formed or the quantity in which oxygen is used.
Abstract translation: 将胆固醇转化为DELTA4-胆甾烯酮和过氧化氢的酶制剂从属于分枝杆菌属的诺卡氏菌属获得。 该制剂具有每5mg蛋白质氮至少1单位的胆固醇氧化酶比活性，并且当以液体形式时，其效力为至少10 -3单位/ ml。 它们通过生长生物体并回收酶制剂，优选通过用表面活性剂如Triton X-100萃取收获的细胞来制备。 酶制剂用于通过测量胆固醇氧化酶反应的产物之一，优选过氧化氢的形成量或使用氧气的量来测定胆固醇。
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公开(公告)号：US3907642A
公开(公告)日：1975-09-23
申请号：US28958172
申请日：1972-09-15
Applicant: NAT RES DEV
Inventor： RICHMOND WILLIAM
CPC classification number: C12N9/0006 , C12Q1/26 , C12Q1/60 , Y10S210/922 , Y10S435/815 , Y10S435/866 , Y10S435/872 , Y10S435/911 , Y10S435/913 , Y10S435/923 , Y10S435/924 , Y10S435/942
Abstract: Enzyme preparations which will convert cholesterol to Delta 4cholestenone and hydrogen peroxide are obtained from certain Nocardia species belonging to the Mycobacterium rhodocrous group. The preparations have a cholesterol oxidase specific activity of at least 1 unit per 5 mg of protein nitrogen and, when in liquid form, a potency of at least 10 2 units/ml. They are prepared by growing the organism and recovering the enzyme preparation, preferably by extracting the harvested cells with a surface active agent such as Triton X-100. The enzyme preparations are used to assay for cholesterol by measuring the amount in which one of the products of the cholesterol oxidase reaction, preferably hydrogen peroxide, is formed or the quantity in which oxygen is used.
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