公开(公告)号：US20220026424A1

公开(公告)日：2022-01-27

申请号：US17431128

申请日：2020-02-26

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Hyonchol KIM ,  Dai KATO ,  Naoshi KOJIMA ,  Shohei YAMAMURA ,  Tomoyuki KAMATA

IPC: G01N33/543 ,  G01N21/66 ,  G01N33/574 ,  G01N33/553

Abstract: In order to provide a specific solution for producing a microstructure equipped with a mechanism for selectively detecting a marker molecule expressed by a target cell, or a specific biomolecule, and for detecting and identifying a molecule to be detected using the microstructure, the present invention provides a nearly hemispherical shell-shaped structure made of a first conductive material, and an electrode layer made of a second conductive material disposed on the concave side of the nearly hemispherical shell-shaped structure, wherein the first conductive material includes a magnetic material and the second conductive material includes an electrode material, and the size (diameter) of the cavity surrounded by the electrode layer on the concave side of the nearly hemispherical shell-shaped structure is in the range of about 10 nm to about 50 μm.

公开(公告)号：US20180251759A1

公开(公告)日：2018-09-06

申请号：US15579841

申请日：2016-06-08

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Yoshio KATO ,  Naoshi KOJIMA

IPC: C12N15/113 ,  C12N15/67

Abstract: Provided is a method for synthesizing a protein, into which a nucleobase amino acid (NBA) is introduced at a desired position, that comprises: a step for preparing mRNA into which a modified codon is inserted at a desired position downstream of an initiation codon; and a step for translating the aforesaid mRNA into a protein in the presence of tRNA, said tRNA recognizing the modified codon and being acylated with the NBA. Also provided is a ribozyme that catalyzes the aminoacylation of tRNA and comprises two RNA molecules.

公开(公告)号：US20220307063A1

公开(公告)日：2022-09-29

申请号：US17615853

申请日：2020-06-24

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Shunsuke TOMITA ,  Ryoji KURITA ,  Hideyuki TAMAKI ,  Hiroyuki KUSADA ,  Naoshi KOJIMA ,  Sayaka ISHIHARA ,  Koyomi MIYAZAKI ,  Isao YUMOTO

IPC: C12Q1/04 ,  G01N21/64

Abstract: A method for analyzing a microbiota, comprising (1) dissolving a probe capable of non-specifically interacting with a plurality of microorganisms in a plurality of solvents having different ionic strengths and pH levels, wherein the probe comprises: (a) a cationic polymer and (b) an environment-sensitive fluorophore; (2) adding a test sample containing the microbiota to a plurality of probe solutions prepared in the step (1), thereby microorganisms in the test sample and the probe are interacted non-specifically; (3) measuring fluorescence intensities of the plurality of probe solutions to which the test sample has been added in the step (2); and (4) comparing the pattern of fluorescence intensities obtained in the step (3) with the pattern of fluorescence intensities obtained from a reference sample.