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公开(公告)号：US11028373B2
公开(公告)日：2021-06-08
申请号：US16381010
申请日：2019-04-11
发明人： Yougen Li , Mark S. Payne , Jared B. Parker , Slavko Kralj , Veli Alkan , Richard R. Bott , Robert Dicosimo , Qiong Cheng , Ellen D. Semke , Susan Marie Hennessey
摘要： Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
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公开(公告)号：US11952598B2
公开(公告)日：2024-04-09
申请号：US17338842
申请日：2021-06-04
发明人： Yougen Li , Mark S. Payne , Jared B. Parker , Slavko Kralj , Veli Alkan , Richard R. Bott , Robert Dicosimo , Qiong Cheng , Ellen D. Semke , Susan Marie Hennessey
CPC分类号： C12N9/1051 , C08B37/0009 , C12P19/04 , C12P19/18
摘要： Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
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公开(公告)号：US11473116B2
公开(公告)日：2022-10-18
申请号：US17073439
申请日：2020-10-19
发明人： Zheyong Yu , Slavko Kralj , Zhenghong Zhang , Laurie A. Howe
摘要： Reaction compositions are disclosed herein comprising at least water, beta-glucose -1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.
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