公开(公告)号：US20150008340A1

公开(公告)日：2015-01-08

申请号：US14495241

申请日：2014-09-24

Applicant: OLYMPUS CORPORATION

Inventor： Katsumasa FUJITA ,  Takeharu NAGAI ,  Kenta SAITO ,  Masahito YAMANAKA ,  Shinichi TAKIMOTO

IPC: G01N21/64

CPC classification number: G02B21/0076 ,  A61B6/037 ,  A61B6/0478 ,  A61B6/501 ,  G01N21/6458 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2201/068 ,  G01N2201/0697 ,  G01T1/2985

Abstract: A fluorescence observation method of the present invention for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules includes: subjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules; and simultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light.

Abstract translation: 本发明的用于检测从两种以上荧光分子发出的多种荧光的荧光观察方法包括：通过激发具有激发波长的光，使两种或更多种荧光分子中的每一种进行多光子激发 或者在可见光区域中短于700nm，在使用两种或更多种荧光分子的深紫外区域中的吸收波长带产生荧光; 并且同时检测在激发光的激发波长的短波长侧或短波长侧和长波长侧的两端产生的多种荧光。