公开(公告)号：US20190219810A1

公开(公告)日：2019-07-18

申请号：US16364946

申请日：2019-03-26

Applicant: OLYMPUS CORPORATION

Inventor： Masaru MIZUNAKA ,  Shintaro TAKAHASHI ,  Akira MATSUSHITA ,  Yohei TANIKAWA ,  Tsuyoshi MOCHIZUKI ,  Shinichi TAKIMOTO

IPC: G02B21/16 ,  G02B21/02 ,  G02B21/12 ,  G02B21/36 ,  G01N21/64

CPC classification number: G02B21/16 ,  G01N21/17 ,  G01N21/64 ,  G01N21/6458 ,  G01N2021/6478 ,  G02B21/02 ,  G02B21/06 ,  G02B21/10 ,  G02B21/12 ,  G02B21/125 ,  G02B21/24 ,  G02B21/33 ,  G02B21/36 ,  G02B21/367

Abstract: An observation apparatus including: a light-source that emits illumination light and excitation light upward from below a specimen; and an image-capturing optical system having an objective lens that focuses, below the specimen, transmitted light, which is the illumination light that is emitted from the light-source, that is reflected above the specimen, and that has passed through the specimen, and fluorescence that is generated in the specimen that has been irradiated with the excitation light emitted from the light-source, wherein the light-source is disposed radially outside the objective lens.

公开(公告)号：US20200175254A1

公开(公告)日：2020-06-04

申请号：US16785714

申请日：2020-02-10

Applicant: OLYMPUS CORPORATION

Inventor： Shinichi TAKIMOTO ,  Yohei TANIKAWA ,  Taiji MINE ,  Takayuki NAKATOMI ,  Masakazu FUJII

IPC: G06K9/00 ,  G06K9/32 ,  G06F16/78

Abstract: An observation system includes a processor. The processor manages a plurality of images acquired in time series with respect to a biological sample which is being cultured, records a first comment and a first region of interest (RoI) in a storage device in association with the images based on user's instructions, and records a second comment and a second ROI in the storage device in association with the first comment based on user's instructions.

公开(公告)号：US20170010453A1

公开(公告)日：2017-01-12

申请号：US15275359

申请日：2016-09-24

Applicant: OLYMPUS CORPORATION

Inventor： Kentaro IMOTO ,  Shinichi TAKIMOTO ,  Shintaro TAKAHASHI ,  Atsushi DOI

IPC: G02B21/00 ,  G01N21/64

CPC classification number: G02B21/0076 ,  G01N21/6458 ,  G01N2201/06113 ,  G01N2201/1053 ,  G01N2201/12 ,  G02B21/0032 ,  G02B21/006 ,  G02B21/0068 ,  G02B21/008 ,  G02B21/0084

Abstract: Provided is a microscope provided with: a scanner that scans an excitation beam coming from a light source; an objective optical system that focuses the scanned excitation beam onto a sample and that collects fluorescence generated at individual scanning positions in the sample; a detector that detects the collected fluorescence; a light blocking member that is disposed between the detector and the system and that partially blocks the collected fluorescence; a switching portion that switches the positional relationship between the member and a light-focusing point of the excitation beam in the sample between an optically conjugate positional relationship, in which an in-focus fluorescence generated at the light-focusing point passes through the member, and a non-conjugate positional relationship, in which the in-focus fluorescence is blocked by the member; and a computing portion that computes a difference between fluorescence signals acquired by the detector in the two positional relationships.

Abstract translation: 提供了一种显微镜，其具有：扫描来自光源的激发光束的扫描仪; 物镜光学系统，其将扫描的激发光束聚焦到样品上并且收集样品中各个扫描位置产生的荧光; 检测所收集的荧光的检测器; 光阻挡构件，其设置在所述检测器和所述系统之间并部分阻挡所述收集的荧光; 切换部，其以在所述光聚合点处产生的聚焦荧光透过所述构件的光学共轭位置关系之间切换所述构件与所述样品中的所述激发光束的聚光点之间的位置关系， 以及非共轭位置关系，其中聚焦荧光被构件阻挡; 以及计算部分，其计算由两个位置关系中的检测器获取的荧光信号之间的差异。

公开(公告)号：US20160349181A1

公开(公告)日：2016-12-01

申请号：US15233230

申请日：2016-08-10

Applicant: OLYMPUS CORPORATION

Inventor： Akira SATO ,  Shinichi TAKIMOTO

IPC: G01N21/65 ,  G01J3/44 ,  G01J3/42

CPC classification number: G01N21/65 ,  G01J3/28 ,  G01J3/42 ,  G01J3/44 ,  G01N21/35 ,  G01N2201/1293

Abstract: With a spectroscopic analysis method of the present invention, by performing principal component analysis on optical spectra measured at individual positions of a specimen, principal components of a plurality of orders that constitute the individual optical spectra are calculated; for the individual orders, principal-component images in which values thereof are principal-component scores of the individual principal components corresponding to the positions are created; distribution patterns of the principal-component scores are identified in the individual principal-component images; the morphology of the specimen is identified in a morphological image in which the specimen is captured; the principal-component images that have principal-component-score distribution patterns correlated with the morphology of the specimen are identified; and the individual optical spectra are reconstructed by using the principal components of orders corresponding to the orders of the identified principal-component images.

Abstract translation: 利用本发明的光谱分析方法，通过对在试样的各个位置测定的光谱进行主成分分析，算出构成各个光谱的多个次序的主成分; 对于各个订单，产生其中其值为与位置对应的各个主要分量的主分量分数的主分量图像; 主成分分数的分布模式在各个主成分图像中被识别; 在其中捕获样本的形态图像中鉴定样品的形态; 确定了具有与样本形态相关的主分量分布模式的主成分图像; 并且通过使用与所识别的主分量图像的顺序相对应的次序的主成分来重建各个光谱。

公开(公告)号：US20190333215A1

公开(公告)日：2019-10-31

申请号：US16458397

申请日：2019-07-01

Applicant: OLYMPUS CORPORATION

Inventor： Naohiro ARIGA ,  Shintaro TAKAHASHI ,  Yohei TANIKAWA ,  Shinichi TAKIMOTO

IPC: G06T7/00 ,  G01N33/483 ,  G06F17/18

Abstract: A cell observation system includes an imaging element that acquires images of the inside of a culture container in which cells are cultured, the imaging element acquiring the images over time; a computer configured to: quantitatively analyze a culture state of the cells cultured in the culture container on the basis of each of the images acquired by the imaging element; and statistically analyze the quantitatively analyzed data; and a display that displays statistical analysis results in the culture container within a plurality of subculture periods obtained by the computer in a manner allowing comparison of the statistical analysis results.

公开(公告)号：US20180032787A1

公开(公告)日：2018-02-01

申请号：US15725413

申请日：2017-10-05

Applicant: OLYMPUS CORPORATION

Inventor： Yasunobu IGA ,  Shinichi TAKIMOTO ,  Yohei TANIKAWA ,  Yoshinobu AKAHORI

IPC: G06K9/00 ,  G06T7/13 ,  G06T7/00 ,  C12Q1/06

CPC classification number: G06K9/0014 ,  C12M1/00 ,  C12M41/36 ,  C12Q1/06 ,  G01N2015/0693 ,  G01N2015/1486 ,  G06K9/00134 ,  G06T7/0002 ,  G06T7/13 ,  G06T2207/30024 ,  G06T2207/30168 ,  G06T2207/30242

Abstract: For the purpose of counting the number of cells within a culture container readily and accurately, a cell analysis device includes a cell-image acquiring unit that acquires an image of cells within a culture container in which the cells are cultured, a usability determining unit that determines whether or not the image acquired by the cell-image acquiring unit is usable, a number-of-cells counting unit that counts the number of cells within the image determined as being usable by the usability determining unit, and a number-of-cells calculating unit that calculates the number of cells within the culture container based on the number of cells counted by the number-of-cells counting unit.

公开(公告)号：US20170284938A1

公开(公告)日：2017-10-05

申请号：US15631358

申请日：2017-06-23

Applicant: OLYMPUS CORPORATION

Inventor： Shinichi TAKIMOTO ,  Yohei TANIKAWA

IPC: G01N21/64 ,  G02B21/18

CPC classification number: G01N21/64 ,  A61B1/043 ,  A61B5/0071 ,  A61B5/4064 ,  G01N21/6456 ,  G01N2021/6484 ,  G02B21/16 ,  G02B21/18

Abstract: Provided is a biological tissue observation apparatus including an optical fiber probe that receives fluorescence via an input end disposed at one end thereof and that optically guides the fluorescence toward the base end in the longitudinal direction, a photodetector that is disposed facing an output end disposed at the base end of the optical fiber probe and that detects the fluorescence output from the output end, and a scatter suppressing unit provided near the input end of the optical fiber probe.

公开(公告)号：US20170177789A1

公开(公告)日：2017-06-22

申请号：US15405545

申请日：2017-01-13

Applicant: OLYMPUS CORPORATION

Inventor： Yasunobu IGA ,  Yohei TANIKAWA ,  Shinichi TAKIMOTO ,  Yoshinobu AKAHORI

IPC: G06F19/22 ,  G06F17/30 ,  G06F19/00 ,  C12M1/34 ,  G01N21/27

Abstract: A cell observation information processing system includes: a tag acquiring section that acquires, as a search tag for searching for observation information acquired in response to an acquisition order at one time point, tags containing a cell name, first culture tool identifying information for identifying a culture tool for culturing cells under observation, and second culture tool identifying information for identifying a passage-source culture tool; a storing section that stores in an archive section each piece of the observation information acquired at each time point upon assigning thereto the search tag; a search criterion acquiring section that acquires, as a search criterion, tags containing a cell name and first culture tool identifying information; and a retrieving section that searches through the archive section using the acquired search criterion and retrieves search-tagged observation information on cells having undergone passage of culture from a single cell line over a plurality of generations.

公开(公告)号：US20170098033A1

公开(公告)日：2017-04-06

申请号：US15292581

申请日：2016-10-13

Applicant: OLYMPUS CORPORATION

Inventor： Yasunobu IGA ,  Yohei TANIKAWA ,  Shinichi TAKIMOTO

IPC: G06F19/28

CPC classification number: G16B50/00 ,  C12M1/34 ,  G01N33/48 ,  G06F16/5866

Abstract: A cell observation information processing system includes: a tag acquiring section that acquires a tag set for searching for observation information acquired via an acquisition order at one time point; and a storing section that stores into an archive the observation information upon assigning a tag set thereto. The tag acquiring section automatically acquires or generates a possible tag set suggested to be assigned to the observation information as well as accepts an order to change the possible tag set to another tag set from a user, and, if having not received an order to change, acquires the possible tag set as a tag set to be assigned without accepting an input of another tag set by the user, or, if having received an order to change, accepts an input of another tag set by the user and acquires the accepted another tag set as a tag set to be assigned.

公开(公告)号：US20160203186A1

公开(公告)日：2016-07-14

申请号：US15079916

申请日：2016-03-24

Applicant: OLYMPUS CORPORATION

Inventor： Yohei TANIKAWA ,  Yasunobu IGA ,  Shinichi TAKIMOTO

IPC: G06F17/30 ,  G06K9/00

CPC classification number: G06F16/2455 ,  G06F16/434 ,  G06F16/51 ,  G06F16/5866 ,  G06K9/00147 ,  G16H10/40 ,  G16H30/40

Abstract: A cell observation information processing system includes: a tag acquiring section that acquires, for searching for observation information acquired at one time point, at least one of search tags including tags respectively indicating user identifying information, cell identifying information, information on maintenance work, apparatus identifying information, activity data variation information, and cell image; a storing section that assigns the search tags acquired by the tag acquiring section to each set of the observation information acquired at each time point and stores into an archive section each set of the search-tagged observation information; a search criterion acquiring section that acquires, as a search criterion, information containing at least one of the search tags assigned to the observation information; and a retrieving section that searches through the archive section using the search criterion and retrieves data containing search-tagged observation information to which search tags matched with the search criterion have been assigned.

Abstract translation: 小区观测信息处理系统包括：标签获取部分，用于搜索在一个时间点获取的观察信息，包括分别指示用户识别信息的标签的检索标签，小区识别信息，维护工作信息，设备 识别信息，活动数据变化信息和细胞图像; 存储部分，其将由标签获取部分获取的搜索标签分配给在每个时间点获取的每组观察信息，并将各组搜索标记的观察信息存储在归档部分中; 搜索条件获取部分，其获取包含分配给所述观察信息的所述搜索标签中的至少一个的作为搜索准则的信息; 以及检索部分，其使用搜索条件搜索归档部分，并且检索包含搜索标记的观察信息的数据，其中已经分配了与搜索条件匹配的搜索标签。