公开(公告)号：US20190242824A1

公开(公告)日：2019-08-08

申请号：US16258703

申请日：2019-01-28

申请人： Xidian University

发明人： Xueli CHEN ,  Yonghua ZHAN ,  Nan WANG ,  Honghao CAO ,  Duofang CHEN ,  Shouping ZHU ,  Jimin LIANG

IPC分类号： G01N21/64 ,  G01N21/47

CPC分类号： G01N21/6486 ,  G01N21/4795 ,  G01N21/6458 ,  G01N2021/1787

摘要： The invention discloses a two-mode Raman optical projection tomography system. Samples are irradiated by the laser beam after the beam being expanded by beam expander. Optical signal of each mode will be separated by the beam splitter. Sparse sampling method is used for signal collection. Optical transmission projection signal acquisition module collects transmitted light of samples to form optical projection image. Multi-spectral Raman scattering signal acquisition module collects Raman scattering light produced by samples. Background noise is removed from the collected data. Sparse sampling data are reconstructed by using algebraic reconstruction method (ART) based on TV minimization. The three-dimensional structure image obtained by reconstruction and the three-dimensional chemical compositions image are fused to obtain the three-dimensional volume image with multiple information.

公开(公告)号：US20190234874A1

公开(公告)日：2019-08-01

申请号：US16319099

申请日：2017-07-19

申请人： ALTIUS INSTITUTE FOR BIOMEDICAL SCIENCES ,  UNIVERSITY OF WASHINGTON

发明人： John Stamatoyannopoulos ,  Shreeram AKILESH

IPC分类号： G01N21/64 ,  G01N21/78 ,  C12P19/34 ,  A01N1/02 ,  C12N5/09 ,  C12N5/078 ,  G01N1/30

CPC分类号： G01N21/64 ,  A01N1/0221 ,  C12N5/0634 ,  C12N5/0694 ,  C12P19/34 ,  C12Q1/6841 ,  C40B30/04 ,  C40B40/06 ,  G01N1/30 ,  G01N21/6458 ,  G01N21/78 ,  G01N33/56966 ,  G01N33/574 ,  G01N33/582 ,  G01N2015/0038 ,  C12Q2521/301 ,  C12Q2563/107

摘要： Disclosed herein are methods of detecting a regulatory element, determining the localization of a regulatory element, and/or measuring the activity of a regulatory element.

公开(公告)号：US20190212271A1

公开(公告)日：2019-07-11

申请号：US16245241

申请日：2019-01-10

申请人： Mikhail Zamkov

发明人： Mikhail Zamkov

IPC分类号： G01N21/64 ,  G02B21/16

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N21/6447 ,  G01N2021/6439 ,  G01N2021/6471 ,  G02B21/16

摘要： A method and apparatus for detecting more than one fluorophore in a sample. A broad spectrum excitation light is passed through a shaping filter before illuminating the target sample with more than one fluorophore to be detected, where the optical density of the shaping filter changes based on the wavelength of the broad-spectrum excitation light passing through it.

公开(公告)号：US20190204609A1

公开(公告)日：2019-07-04

申请号：US16295284

申请日：2019-03-07

申请人： Verily Life Sciences LLC

发明人： Supriyo Sinha ,  Cheng-Hsu Wu

IPC分类号： G02B27/10 ,  G02B5/20 ,  G02B21/00 ,  G01J3/12 ,  G02B27/14 ,  G02B26/00 ,  G01J3/02 ,  G01N21/64 ,  G01N21/31 ,  G01J3/28

CPC分类号： G02B27/1006 ,  G01J3/02 ,  G01J3/12 ,  G01J3/2823 ,  G01J2003/1213 ,  G01N21/31 ,  G01N21/6456 ,  G01N21/6458 ,  G02B5/20 ,  G02B21/0064 ,  G02B26/007 ,  G02B27/10 ,  G02B27/102 ,  G02B27/141

摘要： Systems and methods for filtering an optical beam are described. In one implementation, a system for filtering an input optical beam includes a first beamsplitter, a first spectral slicing module, a second spectral slicing module, and a second beamsplitter. The first beamsplitter is configured to split the input optical beam into a first optical beam and a second optical beam. The first spectral slicing module has a first passband and is configured to filter the first optical beam. The second spectral slicing module has a second passband and is configured to filter the second optical beam. The second beamsplitter is configured to combine the first optical beam and the second optical beam into an output optical beam. The first and second spectral slicing modules may each comprise a longpass filter and a shortpass filter aligned along its optical axis, and the longpass filter and/or the shortpass filter are rotatable relative to the optical axis. Advantageously, the optical system allows for tunable spectral filtering of the input optical beam suitable for 2-D imaging systems.

公开(公告)号：US20190203263A1

公开(公告)日：2019-07-04

申请号：US16249681

申请日：2019-01-16

申请人： 454 Life Sciences Corporation

发明人： Jan Berka ,  Yi-Ju Chen ,  John H. Leamon ,  Steve Lefkowitz ,  Kenton L. Lohman ,  Vinod B. Makhijani ,  Jonathan M. Rothberg ,  Gary J. Sarkis ,  Maithreyan Srinivasan ,  Michael P. Weiner

IPC分类号： C12Q1/686 ,  C12Q1/6874 ,  C12N15/10 ,  B01L3/00 ,  G01N21/25 ,  G01N21/64 ,  C12Q1/6806 ,  G01R33/12 ,  C07H21/00 ,  C12Q1/6869 ,  C12Q1/6867 ,  C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6834

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/5085 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0877 ,  C07H21/00 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6484 ,  G01R33/1269 ,  Y02P20/582 ,  Y10T436/143333 ,  C12Q2565/119 ,  C12Q2531/113 ,  C12Q2565/537 ,  C12Q2547/107 ,  C12Q2547/101 ,  C12Q2537/143 ,  C12Q2527/125 ,  C12Q2525/186 ,  C12Q2565/301 ,  C12Q2563/149 ,  C12Q2531/125 ,  C12Q2537/149 ,  C12Q2535/119

摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

公开(公告)号：US20190187453A1

公开(公告)日：2019-06-20

申请号：US16223511

申请日：2018-12-18

申请人： Commissariat a l'energie atomique et aux energies alternatives

发明人： Ondrej Mandula ,  Cedric Allier

IPC分类号： G02B21/36 ,  G02B21/16 ,  G02B21/06 ,  G01N21/64 ,  G06K9/00

CPC分类号： G02B21/367 ,  G01N21/4788 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/6491 ,  G02B21/06 ,  G02B21/16 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/005 ,  G03H2001/0447 ,  G03H2001/0452 ,  G06K9/00127

摘要： The invention relates to a method for observing a fluorescent sample (10), lying in a plane (P10), called the sample plane, the sample comprising a fluorescent agent (10f) able to emit a fluorescence light wave (Δf), in a fluorescence spectral band (Δλf), when it is illuminated by an excitation light wave (Δe), in an excitation spectral band (Δλe), the method comprising the following steps: a) illuminating the sample (10) using a first light source (11), in a first illumination spectral band (Δλ1), in the excitation spectral band (Δλe0, and acquiring a first image (I1) of the sample, in the fluorescence spectral band, using an image sensor (30); b) illuminating the sample (10) using a second light source (12), in a second spectral band (Δλ2), outside of the fluorescence spectral band, and acquiring a second image (I2) of the sample, in the second apectral band, using the image sensor; the image sensor (30) being coupled to an optical system (20) such that: in the fluorescence spectral band (αλf), the object focal plane (P1) of the optical system is coincident with the plane (P10) of the sample; in the second spectral band, the object focal plane (P2) of the optical system is offset with respect to the plane (P10) of the sample, the offset being larger than 20 μm.

公开(公告)号：US20190187054A1

公开(公告)日：2019-06-20

申请号：US16323244

申请日：2017-08-09

申请人： ATILA BIOSYSTEMS, INC. ,  SYNERGISTIC DETECTOR DESIGNS, LLC

发明人： Arthur H. ROGERS ,  James T. ALCIVAR

IPC分类号： G01N21/64 ,  G01N21/03

CPC分类号： G01N21/6452 ,  G01N21/0332 ,  G01N21/6456 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2201/0627

摘要： Various embodiments are directed to a fluorescence reader for assessing a fluorescence intensity of one or more substance samples. The fluorescence reader may comprise a sample support structure for accepting sample wells containing corresponding samples therein, an illumination structure positioned below the sample support structure and configured to illuminate one or more samples positioned within the sample wells, and an imaging structure positioned above the sample support structure and configured to detect a fluorescence emitted by the one or more samples. The illumination structure may comprise one or more reflective perimeter walls, together with the support structure, defining an enclosed illumination cavity, and one or more light sources positioned within the enclosed illumination cavity and configured to emit light causing the one or more samples to fluoresce.

公开(公告)号：US20190145882A1

公开(公告)日：2019-05-16

申请号：US16247426

申请日：2019-01-14

申请人： The Regents of the University of California

发明人： Bahram Jalali ,  Eric Diebold ,  Brandon Buckley

IPC分类号： G01N15/14 ,  G01N21/64 ,  G01N33/537

CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N33/537 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2021/6421 ,  G01N2201/067

摘要： An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.

公开(公告)号：US20190086262A1

公开(公告)日：2019-03-21

申请号：US16091837

申请日：2017-04-03

申请人： HORIBA FRANCE SAS

发明人： Simon RICHARD ,  Sébastien LADEN ,  Dmitri KOUZNETSOV

IPC分类号： G01J3/28 ,  G01J3/44 ,  G01N21/64 ,  G01N21/65 ,  G06T3/40

CPC分类号： G01J3/2823 ,  G01J3/027 ,  G01J3/2803 ,  G01J3/44 ,  G01J3/4406 ,  G01J2003/2813 ,  G01J2003/2826 ,  G01N21/6458 ,  G01N21/65 ,  G06T3/4061

摘要： Disclosed is a method for acquiring and forming a spectrometry image, including the following steps: a) acquiring an initial structural image of an area of a sample; b) breaking down the initial structural image so as to determine a multi-scale spatial sample of the area of the sample; c) determining a plurality of spectrometry measurement positions in the area of the sample, as a function of the multi-scale spatial sampling determined in step b); d) consecutively, for each spectrometry measurement position determined in step c), positioning the excitation beam and acquiring a spectrometry measurement; and e) reconstructing a spectrometry image point-by-point from the spectrometry measurements acquired in step d).

公开(公告)号：US20190049382A1

公开(公告)日：2019-02-14

申请号：US16079900

申请日：2017-02-23

申请人： Single Technologies AB

发明人： Raoul Stubbe ,  Bengt Sahlgren ,  Johan Strömqvist

IPC分类号： G01N21/64 ,  G01N21/51 ,  G02B19/00 ,  G02B21/00 ,  G02B21/26 ,  G02B21/33 ,  G02B27/10 ,  G02B27/14

CPC分类号： G01N21/6458 ,  G01N21/474 ,  G01N21/51 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2021/6482 ,  G01N2021/6484 ,  G01N2201/0833 ,  G01N2201/103 ,  G02B19/0019 ,  G02B19/0057 ,  G02B21/0032 ,  G02B21/0036 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/26 ,  G02B21/33 ,  G02B27/1006 ,  G02B27/141

摘要： An apparatus for imaging a includes an light source for illuminating the sample simultaneously in a line focus or an array of foci; and a sensor for detecting photons emitted or scattered from the sample simultaneously in an array of fields of view. An array of sub-observation volumes in the sample, from which photons are emitted or scattered during imaging, is defined by the volumes in space where the line focus or array of foci from the light source overlap with the corresponding array of field of views of the sensor. A cylindrical sample holder holds the sample at a surface and is rotatably arranged such that at least a portion of the sample can be transported through at least one of the sub-observation volumes by rotating the sample holder. The apparatus can be used in a high-throughput method of imaging.