公开(公告)号：US20040171039A1

公开(公告)日：2004-09-02

申请号：US10716063

申请日：2003-11-17

Applicant: Quantum Dot Corporation

Inventor： Marcel P. Bruchez ,  Jennifer Lai ,  Vince Phillips ,  Andrew R. Watson ,  Edith Wong

IPC: C12Q001/68

CPC classification number: B82Y5/00 ,  B82Y10/00 ,  C12Q1/6818 ,  C12Q1/6834 ,  C12Q2537/143 ,  C12Q2561/119 ,  C12Q2525/301

Abstract: Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide and/or an amplification product therefrom are provided. The methods comprise contacting a sample suspected of containing the target polynucleotide with a polynucleotide that can bind specifically thereto; this polynucleotide is conjugated to a substrate, preferably an encoded bead conjugate. An amplification reaction can first be used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay for the target polynucleotide. An amplification product detection complex and method of forming the same are also provided. The methods are particularly useful in multiplex settings where a plurality of targets are present. Amplification product assay complexes and amplification product assay arrays are also provided, along with methods of forming the same. Kits comprising reagents for performing such methods are also provided.

Abstract translation: 提供了用于测定靶多核苷酸和/或其扩增产物的样品的方法，组合物和制品。 所述方法包括使怀疑含有靶多核苷酸的样品与可特异结合的多核苷酸接触; 该多核苷酸与底物缀合，优选编码的珠缀合物。 首先可以使用扩增反应来产生来自目标多核苷酸的扩增产物，使得其可以用于间接测定靶多核苷酸。 还提供了扩增产物检测复合物及其形成方法。 这些方法在存在多个目标的多路复用设置中特别有用。 还提供了扩增产物测定复合物和扩增产物测定阵列，以及其形成方法。 还提供了包含用于执行这些方法的试剂的试剂盒。

公开(公告)号：US20020028457A1

公开(公告)日：2002-03-07

申请号：US09882193

申请日：2001-06-13

Applicant: Quantum Dot Corporation

Inventor： Stephen Alexander Empedocles ,  Andrew R. Watson ,  Vince Phillips ,  Edith Wong

IPC: C12Q001/68

CPC classification number: B82Y15/00 ,  G01N33/588

Abstract: The present invention provides assays that allow for the detection of a single copy of a target of interest. The target species is either directly or indirectly labeled with a semiconductor nanocrytal, nullquantum dot.null The bright and tunable fluorescence of the quantum dot is readily detected using methods described herein. Also provided are assays that are based on the colocalization of two or more differently colored quantum dots on a single target species, which provides superbly sensitive assays in which the decrease in assay sensitivity caused by non-specific binding of assay mixture components to the assay substrate is minimized. The assays are of use to detect target species including, but are not limited to, nucleic acids, polypeptides, small organic bioactive agents (e.g., drugs, agents of war, herbicides, pesticides, etc.) and organisms.

Abstract translation: 本发明提供允许检测感兴趣的靶标的单拷贝的测定。 目标物种直接或间接用半导体纳米碳“”量子点“标记。 使用本文所述的方法容易地检测量子点的明亮和可调谐的荧光。 还提供了基于在单个靶物种上两种或更多种不同颜色的量子点共定位的测定法，其提供了非常敏感的测定，其中由测定混合物组分非特异性结合到测定底物引起的测定灵敏度降低 被最小化。 该测定法用于检测靶物种，包括但不限于核酸，多肽，小有机生物活性剂（例如药物，战争剂，除草剂，农药等）和生物体。