公开(公告)号：US20040171039A1

公开(公告)日：2004-09-02

申请号：US10716063

申请日：2003-11-17

Applicant: Quantum Dot Corporation

Inventor： Marcel P. Bruchez ,  Jennifer Lai ,  Vince Phillips ,  Andrew R. Watson ,  Edith Wong

IPC: C12Q001/68

CPC classification number: B82Y5/00 ,  B82Y10/00 ,  C12Q1/6818 ,  C12Q1/6834 ,  C12Q2537/143 ,  C12Q2561/119 ,  C12Q2525/301

Abstract: Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide and/or an amplification product therefrom are provided. The methods comprise contacting a sample suspected of containing the target polynucleotide with a polynucleotide that can bind specifically thereto; this polynucleotide is conjugated to a substrate, preferably an encoded bead conjugate. An amplification reaction can first be used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay for the target polynucleotide. An amplification product detection complex and method of forming the same are also provided. The methods are particularly useful in multiplex settings where a plurality of targets are present. Amplification product assay complexes and amplification product assay arrays are also provided, along with methods of forming the same. Kits comprising reagents for performing such methods are also provided.

Abstract translation: 提供了用于测定靶多核苷酸和/或其扩增产物的样品的方法，组合物和制品。 所述方法包括使怀疑含有靶多核苷酸的样品与可特异结合的多核苷酸接触; 该多核苷酸与底物缀合，优选编码的珠缀合物。 首先可以使用扩增反应来产生来自目标多核苷酸的扩增产物，使得其可以用于间接测定靶多核苷酸。 还提供了扩增产物检测复合物及其形成方法。 这些方法在存在多个目标的多路复用设置中特别有用。 还提供了扩增产物测定复合物和扩增产物测定阵列，以及其形成方法。 还提供了包含用于执行这些方法的试剂的试剂盒。

公开(公告)号：US20020144644A1

公开(公告)日：2002-10-10

申请号：US10032809

申请日：2001-12-26

Applicant: Quantum Dot Corporation

Inventor： Donald A. Zehnder ,  Marcel P. Bruchez ,  Joseph A. Treadway ,  Jonathan P. Earhart

IPC: C30B023/00 ,  C30B025/00 ,  C30B028/12

CPC classification number: B82Y30/00 ,  B82Y10/00 ,  C30B7/00 ,  C30B29/605 ,  Y10T117/10

Abstract: Nanocrystals are synthesized with a high degree of control over reaction conditions and hence product quality in a flow-through reactor in which the reaction conditions are maintained by on-line detection of characteristic properties of the product and by adjusting the reaction conditions accordingly. The coating of nanocrystals is achieved in an analogous manner.

Abstract translation: 合成了对反应条件的高度控制，从而在流通反应器中高度控制纳米晶体，其中通过在线检测产物的特征性质并通过相应地调节反应条件来维持反应条件。 以类似的方式实现纳米晶体的涂层。

公开(公告)号：US20040247517A1

公开(公告)日：2004-12-09

申请号：US10726716

申请日：2003-12-02

Applicant: Quantum Dot Corporation, a corporation of the state of California

Inventor： Donald A. Zehnder ,  Marcel P. Bruchez ,  Joseph A. Treadway ,  Jonathan P. Earhart

IPC: C01B019/04 ,  H01L021/00 ,  C01B019/00 ,  C01B025/14

CPC classification number: B82Y30/00 ,  B82Y10/00 ,  C30B7/00 ,  C30B29/605 ,  Y10T117/10

Abstract: Nanocrystals are synthesized with a high degree of control over reaction conditions and hence product quality in a flow-through reactor in which the reaction conditions are maintained by on-line detection of characteristic properties of the product and by adjusting the reaction conditions accordingly. The coating of nanocrystals is achieved in an analogous manner.

公开(公告)号：US20040166505A1

公开(公告)日：2004-08-26

申请号：US10631573

申请日：2003-07-30

Applicant: Quantum Dot Corporation

Inventor： Marcel P. Bruchez ,  R. Hugh Daniels ,  Stephen A. Empedocles ,  Vince E. Phillips ,  Edith Y. Wong ,  Donald A. Zehnder

IPC: C12Q001/68

CPC classification number: G01N33/54346 ,  B82Y15/00 ,  G01N33/533 ,  G01N33/588 ,  G01N2458/00 ,  Y10S977/924

Abstract: The use of semiconductor nanocrystals as detectable labels in various chemical and biological applications is disclosed. The methods find use for detecting a single analyte, as well as multiple analytes by using more than one semiconductor nanocrystal as a detectable label, each of which emits at a distinct wavelength.

Abstract translation: 公开了在各种化学和生物应用中使用半导体纳米晶体作为可检测标记。 该方法用于通过使用多于一种半导体纳米晶体作为可检测标记来检测单个分析物以及多个分析物，每个分析物以不同的波长发射。

公开(公告)号：US20010034034A1

公开(公告)日：2001-10-25

申请号：US09887914

申请日：2001-06-21

Applicant: Quantum Dot Corporation

Inventor： Marcel P. Bruchez ,  R. Hugh Daniels ,  Stephen A. Empedocles ,  Vince E. Phillips ,  Edith Y. Wong ,  Donald A. Zehnder

IPC: C12Q001/68

CPC classification number: G01N33/54346 ,  B82Y15/00 ,  G01N33/533 ,  G01N33/588 ,  G01N2458/00 ,  Y10S977/924

Abstract: The use of semiconductor nanocrystals as detectable labels in various chemical and biological applications is disclosed. The methods find use for detecting a single analyte, as well as multiple analytes by using more than one semiconductor nanocrystal as a detectable label, each of which emits at a distinct wavelength.