-
公开(公告)号：US20120258877A1
公开(公告)日：2012-10-11
申请号：US13024272
申请日：2011-02-09
申请人： Robert M. POPE , Xiquan Liang , Mahbod Hajivandi , John Leite
发明人： Robert M. POPE , Xiquan Liang , Mahbod Hajivandi , John Leite
CPC分类号： G01N33/57415 , G01N33/57496 , G01N33/60 , Y10T436/143333
摘要： Methods are provided for identifying proteins that are differentially expressed in disease state and normal cells. Stable isotope labeling of cells in culture allows for the identification of a multiplicity of proteins whose differential abundance in normal and disease state cells can be indicative of the disease state. Biomarkers are identified for breast cancer, in which the biomarkers are proteins having a two-fold or greater difference in abundance between breast cancer and normal cells. Identified biomarkers can be used detection methods that can provide diagnosis, typing, staging, or prognosis of cancer, such as breast cancer, or can be used to predict the response of cancer, such as breast cancer, to one or more anti-cancer agents.
摘要翻译： 提供了用于鉴定在疾病状态和正常细胞中差异表达的蛋白质的方法。 培养物中细胞的稳定同位素标记允许鉴定多种蛋白质，其正常和疾病状态细胞中的差异丰度可以指示疾病状态。 鉴定乳腺癌的生物标志物，其中生物标志物是乳腺癌和正常细胞之间丰度差异大于2倍或更大的蛋白质。 可以使用识别的生物标志物可以提供诸如乳腺癌的癌症的诊断，分型，分期或预后的检测方法，或者可以用于预测癌症如乳腺癌对一种或多种抗癌剂的反应 。
-
公开(公告)号：US20090226913A1
公开(公告)日：2009-09-10
申请号：US12340341
申请日：2008-12-19
申请人： Robert M. Pope , Xiquan Liang , Mahbod Hajivandi , John Leite
发明人： Robert M. Pope , Xiquan Liang , Mahbod Hajivandi , John Leite
IPC分类号： C12Q1/68 , C12Q1/00 , G01N33/574 , G01N33/00
CPC分类号： G01N33/57415 , G01N33/57496 , G01N33/60 , Y10T436/143333
摘要： Methods are provided for identifying proteins that are differentially expressed in disease state and normal cells. Stable isotope labeling of cells in culture allows for the identification of a multiplicity of proteins whose differential abundance in normal and disease state cells can be indicative of the disease state. Biomarkers are identified for breast cancer, in which the biomarkers are proteins having a two-fold or greater difference in abundance between breast cancer and normal cells. Identified biomarkers can be used detection methods that can provide diagnosis, typing, staging, or prognosis of cancer, such as breast cancer, or can be used to predict the response of cancer, such as breast cancer, to one or more anti-cancer agents.
摘要翻译： 提供了用于鉴定在疾病状态和正常细胞中差异表达的蛋白质的方法。 培养物中细胞的稳定同位素标记允许鉴定多种蛋白质，其正常和疾病状态细胞中的差异丰度可以指示疾病状态。 鉴定乳腺癌的生物标志物，其中生物标志物是乳腺癌和正常细胞之间丰度差异大于2倍或更大的蛋白质。 可以使用识别的生物标志物可以提供诸如乳腺癌的癌症的诊断，分型，分期或预后的检测方法，或者可以用于预测癌症如乳腺癌对一种或多种抗癌剂的反应 。
-
3.发明申请公开(公告)号：US20070105181A1
公开(公告)日：2007-05-10
申请号：US11417264
申请日：2006-05-04
申请人： Robert Pope , Xiquan Liang , Mahbod Hajivandi , John Leite
发明人： Robert Pope , Xiquan Liang , Mahbod Hajivandi , John Leite
IPC分类号： C12Q1/37
CPC分类号： G01N33/57415 , G01N33/57496 , G01N33/60 , Y10T436/143333
摘要： Methods are provided for identifying proteins that are differentially expressed in disease state and normal cells. Stable isotope labeling of cells in culture allows for the identification of a multiplicity of proteins whose differential abundance in normal and disease state cells can be indicative of the disease state. Biomarkers are identified for breast cancer, in which the biomarkers are proteins having a two-fold or greater difference in abundance between breast cancer and normal cells. Identified biomarkers can be used detection methods that can provide diagnosis, typing, staging, or prognosis of cancer, such as breast cancer, or can be used to predict the response of cancer, such as breast cancer, to one or more anti-cancer agents.
摘要翻译： 提供了用于鉴定在疾病状态和正常细胞中差异表达的蛋白质的方法。 培养物中细胞的稳定同位素标记允许鉴定多种蛋白质，其正常和疾病状态细胞中的差异丰度可以指示疾病状态。 鉴定乳腺癌的生物标志物，其中生物标志物是乳腺癌和正常细胞之间丰度差异大于2倍或更大的蛋白质。 可以使用识别的生物标志物可以提供诸如乳腺癌的癌症的诊断，分型，分期或预后的检测方法，或者可以用于预测癌症如乳腺癌对一种或多种抗癌剂的反应 。
-
公开(公告)号：US20070031911A1
公开(公告)日：2007-02-08
申请号：US11368996
申请日：2006-03-07
申请人： John Leite , Robert Pope , Mahbod Hajivandi , Xiquan Liang
发明人： John Leite , Robert Pope , Mahbod Hajivandi , Xiquan Liang
CPC分类号： G01N27/62 , G01N33/60 , G01N33/6848 , G01N33/6851 , G01N2458/15 , Y10T436/24
摘要： The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. Methods of the present invention also include methods for quantifying biomolecules where the copy biomolecule standard is made by expressing the biomolecule using a recombinant cell. Further included are the biomolecule standards themselves, method for making such standards, kits, systems, reagents, and engineered cells relating to the use of biomolecule standards in mass spectrometric analysis.
摘要翻译： 本发明提供了在质谱分析中定量生物分子例如多肽的方法。 该方法包括使用具有与感兴趣的生物分子中天然存在的同位素不同的至少一个原子同位素的生物分子标准。 本发明的方法还包括用于定量生物分子的方法，其中通过使用重组细胞表达生物分子来制备复制生物分子标准物。 还包括生物分子标准本身，制备此类标准的方法，试剂盒，系统，试剂和与质谱分析中使用生物分子标准有关的工程细胞。
-
5.发明申请公开(公告)号：US20060023808A1
公开(公告)日：2006-02-02
申请号：US11131744
申请日：2005-05-17
申请人： Mahbod Hajivandi , Robert Pope , Mehrnoosh Sadeghi , Xiquan Liang , John Leite
发明人： Mahbod Hajivandi , Robert Pope , Mehrnoosh Sadeghi , Xiquan Liang , John Leite
IPC分类号： H04L25/49
CPC分类号： H01J49/0009 , C07K14/00 , Y10T436/105831
摘要： The invention provides compositions, kits, and methods for calibrating a mass spectrometer using two or more recombinant proteins and one or more energy-absorbing molecules. The recombinant proteins of the invention display high purities, making them suitable for use in mass spectrometry.
摘要翻译： 本发明提供了使用两种或更多种重组蛋白质和一种或多种能量吸收分子校准质谱仪的组合物，试剂盒和方法。 本发明的重组蛋白显示出高纯度，使其适合用于质谱。
-
6.发明申请公开(公告)号：US20120205592A1
公开(公告)日：2012-08-16
申请号：US13350643
申请日：2012-01-13
IPC分类号： C09K3/00
CPC分类号： H01J49/0009 , C07K14/00 , Y10T436/105831
摘要： The invention provides compositions, kits, and methods for calibrating a mass spectrometer using two or more recombinant proteins and one or more energy-absorbing molecules. The recombinant proteins of the invention display high purities, making them suitable for use in mass spectrometry.
摘要翻译： 本发明提供了使用两种或更多种重组蛋白质和一种或多种能量吸收分子校准质谱仪的组合物，试剂盒和方法。 本发明的重组蛋白显示出高纯度，使其适合用于质谱。
-
公开(公告)号：US20110262947A1
公开(公告)日：2011-10-27
申请号：US13092593
申请日：2011-04-22
IPC分类号： C12Q1/37
CPC分类号： G01N27/62 , G01N33/60 , G01N33/6848 , G01N33/6851 , G01N2458/15 , Y10T436/24
摘要： The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. Methods of the present invention also include methods for quantifying biomolecules where the copy biomolecule standard is made by expressing the biomolecule using a recombinant cell. Further included are the biomolecule standards themselves, method for making such standards, kits, systems, reagents, and engineered cells relating to the use of biomolecule standards in mass spectrometric analysis.
摘要翻译： 本发明提供了在质谱分析中定量生物分子例如多肽的方法。 所述方法包括使用具有与感兴趣的生物分子中天然存在的同位素不同的至少一个原子同位素的生物分子标准物。 本发明的方法还包括用于定量生物分子的方法，其中通过使用重组细胞表达生物分子来制备复制生物分子标准物。 还包括生物分子标准本身，制备此类标准的方法，试剂盒，系统，试剂和与质谱分析中使用生物分子标准有关的工程细胞。
-
公开(公告)号：US07939331B2
公开(公告)日：2011-05-10
申请号：US11368996
申请日：2006-03-07
CPC分类号： G01N27/62 , G01N33/60 , G01N33/6848 , G01N33/6851 , G01N2458/15 , Y10T436/24
摘要： The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. Methods of the present invention also include methods for quantifying biomolecules where the copy biomolecule standard is made by expressing the biomolecule using a recombinant cell. Further included are the biomolecule standards themselves, method for making such standards, kits, systems, reagents, and engineered cells relating to the use of biomolecule standards in mass spectrometric analysis.
摘要翻译： 本发明提供了在质谱分析中量化生物分子例如多肽的方法。 所述方法包括使用具有与感兴趣的生物分子中天然存在的同位素不同的至少一个原子同位素的生物分子标准物。 本发明的方法还包括用于定量生物分子的方法，其中通过使用重组细胞表达生物分子来制备复制生物分子标准物。 还包括生物分子标准本身，制备此类标准的方法，试剂盒，系统，试剂和与质谱分析中使用生物分子标准有关的工程细胞。
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.029 秒)