公开(公告)号：US10590458B2

公开(公告)日：2020-03-17

申请号：US15442202

申请日：2017-02-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Marquant ,  Michael Reinhardt

IPC: G01N27/327 ,  C12Q1/00 ,  G01N33/487 ,  G01N33/49

Abstract: A method of detecting at least one analyte in a test sample is provided comprising a) contacting the test sample (i) to an active chemistry matrix changing at least one electrochemical property dependent on an enzymatic activity active in the presence of the analyte, the active chemistry matrix contacting a first electrode; and (ii) to an inactive chemistry matrix, the inactive chemistry matrix contacting a second electrode, b) closing an electrical circuit including the first electrode, the second electrode, and the active chemistry matrix and inactive chemistry matrix, followed by determining a first value of the at least one electrochemical property, c) inverting electrical polarity of the electrical circuit of b), followed by determining a second value of the at least one electrochemical property, and d) detecting the at least one analyte based on the first value and on the second value.

公开(公告)号：US20170240945A1

公开(公告)日：2017-08-24

申请号：US15442202

申请日：2017-02-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Marquant ,  Michael Reinhardt

IPC: C12Q1/00 ,  G01N33/49 ,  G01N27/327 ,  G01N33/487

Abstract: A method of detecting at least one analyte in a test sample is provided comprising a) contacting the test sample (i) to an active chemistry matrix changing at least one electrochemical property dependent on an enzymatic activity active in the presence of the analyte, the active chemistry matrix contacting a first electrode; and (ii) to an inactive chemistry matrix, the inactive chemistry matrix contacting a second electrode, b) closing an electrical circuit including the first electrode, the second electrode, and the active chemistry matrix and inactive chemistry matrix, followed by determining a first value of the at least one electrochemical property, c) inverting electrical polarity of the electrical circuit of b), followed by determining a second value of the at least one electrochemical property, and d) detecting the at least one analyte based on the first value and on the second value.

公开(公告)号：US11473118B2

公开(公告)日：2022-10-18

申请号：US16735773

申请日：2020-01-07

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Marquant ,  Michael Reinhardt

IPC: G01N27/327 ,  C12Q1/00 ,  G01N33/487 ,  G01N33/49

Abstract: A method of detecting at least one analyte in a test sample is provided comprising a) contacting the test sample (i) to an active chemistry matrix changing at least one electrochemical property dependent on an enzymatic activity active in the presence of the analyte, the active chemistry matrix contacting a first electrode; and (ii) to an inactive chemistry matrix, the inactive chemistry matrix contacting a second electrode, b) closing an electrical circuit including the first electrode, the second electrode, and the active chemistry matrix and inactive chemistry matrix, followed by determining a first value of the at least one electrochemical property, c) inverting electrical polarity of the electrical circuit of b), followed by determining a second value of the at least one electrochemical property, and d) detecting the at least one analyte based on the first value and on the second value.