公开(公告)号：US11479778B2

公开(公告)日：2022-10-25

申请号：US17285816

申请日：2020-11-30

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda Song ,  Caili Sun ,  Huaiyuan Zhang ,  Wu Yang ,  Meiling Chen

IPC: C12N15/80 ,  C12N9/10

Abstract: The present invention relates to the technical field of gene engineering and particularly relates to a method for constructing non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield, recombinant strain constructed by method, and application of recombinant strain. According to the present invention, a diacylglycerol acyltransferase gene (DGAT) is overexpressed in Mucor circinelloides WJ11 by a homologous recombination technology, and exogenous oil is added for fermentation, such that the non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield is constructed. Compared with the control strain Mc2075, the fat yield of the Mucor circinelloides is increased; and when the diacylglycerol acyltransferase (DGAT) is transformed into the uracil defective type of Mucor circinelloides WJ11, the fatty acid composition changes after fermentation, and the lipid content may reach 53% of dry cell weight after the fermentation condition is optimized.