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公开(公告)号：US20210102227A1
公开(公告)日：2021-04-08
申请号：US17120163
申请日：2020-12-12
Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY
Inventor： Yuanda SONG , Md. Ahsanul Kabir KHAN , Junhuan YANG , Huaiyuan ZHANG , Wu YANG , Qing LIU
Abstract: The present disclosure relates to a construction method of a Mucor circinelloides cell factory for producing dihomo-γ-linolenic acid and a fermentation technology, belonging to the field of genetic engineering. In the present disclosure, γ-linolenic acid elongase gene glelo is obtained from Mortierella alpine by cloning, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and the gene glelo is integrated into Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-glelo, and finally, the expression of the gene glelo in Mucor circinelloides is realized. The lipid content in the recombinant strain Mc-glelo is not obviously different from that in the control strain Mc1552, however, the lipid composition changes greatly, and dihomo-γ-linolenic acid appears in the lipids of the recombinant strain Mc-glelo, and the content thereof reaches 5.7% of the total fatty acids. Under optimized fermentation conditions and in the presence of precursor fatty acid, the DGLA content reaches 7.6%. The new recombinant strain was deposited in China General Microbiological Culture Collection Center on Jun. 20, 2018, with the address of No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The accession number given to the biological material by the collection center is CGMCC No. 15887, and the suggested taxonomic denomination is Mucor circinelloides-GLELO.
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公开(公告)号：US20210102225A1
公开(公告)日：2021-04-08
申请号：US17120160
申请日：2020-12-12
Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY
Inventor： Yuanda SONG , Md. Ahsanul Kabir KHAN , Junhuan YANG , Yao ZHANG , Wu YANG , Shaoqi LI
Abstract: Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid SDA and fermentation technology thereof. Δ15 Des gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and Δ15 Des is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-Δ15, finally, the expression of the Δ15 Des gene in Mucor circinelloides is realized. The recombinant new strain was preserved in China General Microbiological Culture Collection Center on Jun. 20, 2018, and the address is NO. 1 West Beichen Road, Chaoyang District, Beijing. The center gave the biological material the preservation number CGMCC No. 15888, and the suggested classification name is Mucor circinelloides-D15D.
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公开(公告)号：US20210292380A1
公开(公告)日：2021-09-23
申请号：US17262960
申请日：2019-11-20
Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY
Inventor： Yuanda SONG , Junhuan YANG , Wu YANG , Shaoqi LI
Abstract: The present application discloses a dicarboxylic acid transporter and its encoding gene dit gene for increasing oil yield of Mucor circinelloides, the dit gene is cloned from the high-yield M. circinelloides WJ11, and the dit gene is transformed into M. circinelloides deficient strain Mu402, the dit gene is integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mc-Dit. The total fatty acid content of the Mc-Dit strain increased by 33.76% and the intracellular lipid content may reach up to 17.67% of the dry biomass.
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公开(公告)号：US20220177526A1
公开(公告)日：2022-06-09
申请号：US17270661
申请日：2019-11-20
Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY
Inventor： Yuanda SONG , Junhuan YANG , Wu YANG
Abstract: A C4-dicarboxylic acid transporter and its encoding gene C4mt gene can increase oil yield of Mucor circinelloides, the C4mt gene may be cloned from the high-yield M. circinelloides WJ11, and the C4mt gene is transformed into M. circinelloides deficient strain Mu402, the C4mt gene can be integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mu-C4mt. The total fatty acid content of the Mu-C4mt strain can be increased by 25.30% and the intracellular lipid content may reach up to 16.34% of the dry biomass.
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