公开(公告)号：US20210292380A1

公开(公告)日：2021-09-23

申请号：US17262960

申请日：2019-11-20

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda SONG ,  Junhuan YANG ,  Wu YANG ,  Shaoqi LI

IPC: C07K14/37 ,  C12N15/80

Abstract: The present application discloses a dicarboxylic acid transporter and its encoding gene dit gene for increasing oil yield of Mucor circinelloides, the dit gene is cloned from the high-yield M. circinelloides WJ11, and the dit gene is transformed into M. circinelloides deficient strain Mu402, the dit gene is integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mc-Dit. The total fatty acid content of the Mc-Dit strain increased by 33.76% and the intracellular lipid content may reach up to 17.67% of the dry biomass.

公开(公告)号：US20220186237A1

公开(公告)日：2022-06-16

申请号：US17285816

申请日：2020-11-30

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda SONG ,  Caili SUN ,  Huaiyuan ZHANG ,  Wu YANG ,  Meiling CHEN

IPC: C12N15/80 ,  C12N9/10

Abstract: The present invention relates to the technical field of gene engineering and particularly relates to a method for constructing non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield, recombinant strain constructed by method, and application of recombinant strain. According to the present invention, a diacylglycerol acyltransferase gene (DGAT) is overexpressed in Mucor circinelloides WJ11 by a homologous recombination technology, and exogenous oil is added for fermentation, such that the non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield is constructed. Compared with the control strain Mc2075, the fat yield of the Mucor circinelloides is increased; and when the diacylglycerol acyltransferase (DGAT) is transformed into the uracil defective type of Mucor circinelloides WJ11, the fatty acid composition changes after fermentation, and the lipid content may reach 53% of dry cell weight after the fermentation condition is optimized.

公开(公告)号：US20210102225A1

公开(公告)日：2021-04-08

申请号：US17120160

申请日：2020-12-12

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda SONG ,  Md. Ahsanul Kabir KHAN ,  Junhuan YANG ,  Yao ZHANG ,  Wu YANG ,  Shaoqi LI

IPC: C12P7/64 ,  C12N9/02

Abstract: Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid SDA and fermentation technology thereof. Δ15 Des gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and Δ15 Des is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-Δ15, finally, the expression of the Δ15 Des gene in Mucor circinelloides is realized. The recombinant new strain was preserved in China General Microbiological Culture Collection Center on Jun. 20, 2018, and the address is NO. 1 West Beichen Road, Chaoyang District, Beijing. The center gave the biological material the preservation number CGMCC No. 15888, and the suggested classification name is Mucor circinelloides-D15D.

公开(公告)号：US20210093688A1

公开(公告)日：2021-04-01

申请号：US17120162

申请日：2020-12-12

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda SONG ,  Syed AMMAR ,  Shaoqi LI ,  Wu YANG

IPC: A61K36/9068 ,  A61K36/537 ,  A61K36/52 ,  A61K36/49 ,  A61K36/54 ,  A61K36/31 ,  A61K36/71 ,  A61K36/287 ,  A61K36/22 ,  A61K36/889 ,  A61K36/63 ,  A61K36/60 ,  A61K36/8962 ,  A61K36/738 ,  A61K36/45 ,  A61K9/14 ,  A61K9/00 ,  A61K9/48

Abstract: The present disclosure provides a natural plant preparation, including extracts of the following parts of plants: roots of Salvia miltiorrhiza Bunge, Juglans regia, Aesculus hippocastanum L. and Zingiber officinale Rosc., barks of Cinnamomum cassia Presl, seeds of Sesamum indicum, Carica papaya L., Nigella damascena L. and Punica granatum L., leaves of Centaurea cyanus L., resin of Pistacia Lentiscus, drupes of Cocos nucifera L., beans of Theobroma cacao L., dried pulp and kernels of Phoenix dactylifera L., dried pulp of Olea europaea, Ficus carica Linn., Walnut and Vaccinium Spp, cloves of Allium sativum, and petals of Rosa sp. The present disclosure further provides a method for preparing a plant preparation, including treating the above parts of plants with ethanol.

公开(公告)号：US20220177526A1

公开(公告)日：2022-06-09

申请号：US17270661

申请日：2019-11-20

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda SONG ,  Junhuan YANG ,  Wu YANG

IPC: C07K14/37 ,  C12N15/80

Abstract: A C4-dicarboxylic acid transporter and its encoding gene C4mt gene can increase oil yield of Mucor circinelloides, the C4mt gene may be cloned from the high-yield M. circinelloides WJ11, and the C4mt gene is transformed into M. circinelloides deficient strain Mu402, the C4mt gene can be integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mu-C4mt. The total fatty acid content of the Mu-C4mt strain can be increased by 25.30% and the intracellular lipid content may reach up to 16.34% of the dry biomass.

公开(公告)号：US20210102227A1

公开(公告)日：2021-04-08

申请号：US17120163

申请日：2020-12-12

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda SONG ,  Md. Ahsanul Kabir KHAN ,  Junhuan YANG ,  Huaiyuan ZHANG ,  Wu YANG ,  Qing LIU

IPC: C12P7/64 ,  C12N15/80 ,  C12N1/14 ,  C12N9/02

Abstract: The present disclosure relates to a construction method of a Mucor circinelloides cell factory for producing dihomo-γ-linolenic acid and a fermentation technology, belonging to the field of genetic engineering. In the present disclosure, γ-linolenic acid elongase gene glelo is obtained from Mortierella alpine by cloning, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and the gene glelo is integrated into Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-glelo, and finally, the expression of the gene glelo in Mucor circinelloides is realized. The lipid content in the recombinant strain Mc-glelo is not obviously different from that in the control strain Mc1552, however, the lipid composition changes greatly, and dihomo-γ-linolenic acid appears in the lipids of the recombinant strain Mc-glelo, and the content thereof reaches 5.7% of the total fatty acids. Under optimized fermentation conditions and in the presence of precursor fatty acid, the DGLA content reaches 7.6%. The new recombinant strain was deposited in China General Microbiological Culture Collection Center on Jun. 20, 2018, with the address of No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The accession number given to the biological material by the collection center is CGMCC No. 15887, and the suggested taxonomic denomination is Mucor circinelloides-GLELO.