公开(公告)号：US20210102225A1

公开(公告)日：2021-04-08

申请号：US17120160

申请日：2020-12-12

Applicant: SHANDONG UNIVERSITY OF TECHNOLOGY

Inventor： Yuanda SONG ,  Md. Ahsanul Kabir KHAN ,  Junhuan YANG ,  Yao ZHANG ,  Wu YANG ,  Shaoqi LI

IPC: C12P7/64 ,  C12N9/02

Abstract: Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid SDA and fermentation technology thereof. Δ15 Des gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and Δ15 Des is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-Δ15, finally, the expression of the Δ15 Des gene in Mucor circinelloides is realized. The recombinant new strain was preserved in China General Microbiological Culture Collection Center on Jun. 20, 2018, and the address is NO. 1 West Beichen Road, Chaoyang District, Beijing. The center gave the biological material the preservation number CGMCC No. 15888, and the suggested classification name is Mucor circinelloides-D15D.