公开(公告)号：US20230036959A1

公开(公告)日：2023-02-02

申请号：US17790934

申请日：2021-01-04

Applicant: SOUTH DAKOTA BOARD OF REGENTS TECHNOLOGY TRANSFER OFFICE

Inventor： Joy Scaria

IPC: C12Q1/04 ,  C12Q1/18 ,  A61K35/742 ,  C12N1/20

Abstract: A composition and method for identifying a microbial composition that inhibits colonization of an enteric pathogen in an animal is disclosed. The method includes removing a microbial sample from a digestive tract of at least one healthy individual, culturing the microbial sample, isolating one or more microbial species within the microbial sample, and identifying one or more isolated microbial species. The method further includes determining one or more bile-tolerant properties of the one or more isolated microbial species, and creating one or more microbial compositions of the one or more isolated microbial species that are bile-tolerant. The method further includes determining an ability of the one or more microbial compositions to inhibit growth of an enteric pathogen in at least one of an assay, and identifying one or more microbial compositions from the one or more microbial compositions capable of inhibiting growth of enteric pathogens in an animal.

公开(公告)号：US20220233612A1

公开(公告)日：2022-07-28

申请号：US17615556

申请日：2020-05-30

Applicant: SOUTH DAKOTA BOARD OF REGENTS TECHNOLOGY TRANSFER OFFICE

Inventor： Joy Scaria

IPC: A61K35/747 ,  C12N1/20 ,  C12Q1/18 ,  A61K35/744 ,  A61K35/742 ,  A61K35/741 ,  A61P31/04

Abstract: A method for identifying a microbial composition that inhibits colonization of an enteric pathogen of a first animal is disclosed. The method includes removing a microbial sample from the digestive tract of a second animal. The method further includes culturing the microbial sample, isolating, cultivating and identifying microbial species within the microbial sample. The method further includes creating compositions of one or more isolated microbial species and determining the ability of the composition to inhibit growth of an enteric pathogen in at least one of an in vitro or an in vivo assay. The method further includes identifying a microbial composition capable of inhibiting growth of enteric pathogens in a first animal. A microbial composition that inhibits colonization of an enteric pathogen of a first animal is also disclosed. The composition includes microorganisms from a group comprising Faecalicoccus, Lactobacillus, Megamonas, Staphylococcus, Bacillus, Enterococcus, Olsenella, Megasphaera, Pseudoflavonifractor, and Massiliomicrobiota.