公开(公告)号：US20160178618A1

公开(公告)日：2016-06-23

申请号：US14973303

申请日：2015-12-17

Applicant: STC.UNM

Inventor： James P. Freyer ,  Andrew P. Shreve ,  Jacqueline DeLora

IPC: G01N33/50 ,  G01N33/574 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00

CPC classification number: C12M29/10 ,  C12M21/08 ,  C12M25/14 ,  C12M35/08 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34 ,  C12M41/38 ,  C12M41/46

Abstract: Disclosed is a perfusion device and methods of use including a generally cylindrical body and a packed bed of nanosensor-cell embedded matrix spheres (nanoCEMS) disposed between layers of inert microspheres. A concentration of a molecule of interest can be established within the perfusion device to effect the cellular and chemical microenvironment of the nanoCEMS, the nanoCEMS in turn creating their own concentration gradients nutrients and waste products in response, which can be measured by nanosensors. The collected measurements can be applied to a transport model to calculate concentrations of various molecules at discreet locations in the perfusion chamber. Also disclosed is a method for making nanoCEMS by mixing a polymeric mixture with a crosslinking solution and dispersed through a nested dispensing device such that mixing of the two mixtures occurs in air. A piezoelectric transducer coupled to the dispensing device controls the droplet formation rate.

Abstract translation: 公开了一种灌注装置和使用方法，包括设置在惰性微球体层之间的纳米传感器 - 电池嵌入式基质球（nanoCEMS）的大体圆柱体和填充床。 可以在灌注装置内建立一个关注分子的浓度，以实现nanoCEMS的细胞和化学微环境，nanoCEMS反过来又产生了自己的浓度梯度营养物质和废物，这可以通过纳米传感器测量。 所收集的测量值可应用于运输模型，以计算灌注腔中谨慎位置处各种分子的浓度。 还公开了通过将聚合物混合物与交联溶液混合并通过嵌套分配装置分散来制备nanoCEMS的方法，使得两种混合物在空气中混合。 耦合到分配装置的压电换能器控制液滴形成速率。