公开(公告)号：US20240287435A1

公开(公告)日：2024-08-29

申请号：US18573974

申请日：2023-05-12

申请人： AQUILA BIOLABS GMBH

发明人： Konrad Herzog ,  Julian Sidram ,  Felix Kortmann

IPC分类号： C12M1/34 ,  C12M1/06

CPC分类号： C12M41/30 ,  C12M27/02 ,  C12M41/26

摘要： Methods and devices for monitoring the content of mixed reactors, where the reactor has at least one property to be monitored that influences at least one signal of at least one sensory component, where the at least one sensory component moves within the reactor so that it is not permanently located in the detection region of the at least one measuring arrangement, and detection is carried out by at least one measuring arrangement while the at least one sensor is located in the detection region.

公开(公告)号：US20240263110A1

公开(公告)日：2024-08-08

申请号：US18410960

申请日：2024-01-11

申请人： POET Research, Inc.

发明人： Jason Alan Bootsma

IPC分类号： C12F3/10 ,  A23K10/38 ,  A23K20/163 ,  A23K50/10 ,  C10L1/02 ,  C11B1/00 ,  C12M1/00 ,  C12M1/34

CPC分类号： C12F3/10 ,  A23K10/38 ,  A23K20/163 ,  A23K50/10 ,  C10L1/026 ,  C11B1/00 ,  C12M41/26 ,  C12M43/00 ,  C12M47/10 ,  C12M47/14 ,  Y02P60/87

摘要： Systems and methods for fractionating whole stillage from an ethanol production facility are provided. Whole stillage undergoes a separation of its liquid portion (thin stillage) from the solid portion (fiber cake). In some embodiments, the solids and liquids in whole stillage may be separated utilizing a screening centrifuge. The fiber cake may be dried to generate a high fiber animal feed. The thin stillage may be provided to a three-phase separator for separation into an oil emulsion, an aqueous clarified stillage, and a protein paste. The protein paste may be dried to generate a high protein animal feed with greater than about 45% protein content. The clarified thin stillage is condensed to yield a syrup with greater than around 60% solids. The oil emulsion is subjected to a pH adjustment to liberate the oil from the emulsion, which is then separated.

公开(公告)号：US12012585B2

公开(公告)日：2024-06-18

申请号：US17699278

申请日：2022-03-21

申请人： TEEWINOT LIFE SCIENCES CORPORATION

发明人： Richard Peet ,  Malcolm J. Kavarana ,  Mingyang Sun ,  Peter C. Michels ,  John D. Rabenstein ,  C. Seth Pearson ,  Geoffrey M. Fox

IPC分类号： C12M1/34 ,  C12M1/00 ,  C12M1/36 ,  C12M1/40 ,  C12P7/42 ,  C12P17/06

CPC分类号： C12M41/30 ,  C12M1/34 ,  C12M1/36 ,  C12M1/40 ,  C12M21/18 ,  C12M23/58 ,  C12M41/12 ,  C12M41/26 ,  C12M41/40 ,  C12M41/48 ,  C12M47/10 ,  C12P7/42 ,  C12P17/06

摘要： The disclosure provides systems and methods for producing a cannabinoid product, which comprises contacting a cannabinoid precursor in a first phase with a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible. The disclosure also provides a composition comprising the cannabinoid precursor in a first phase and a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible.

公开(公告)号：US20240174968A1

公开(公告)日：2024-05-30

申请号：US18284949

申请日：2022-03-30

申请人： CORNING INCORPORATED

发明人： Christopher Bowman Horner ,  Amy Claire Kauffman

IPC分类号： C12M1/34 ,  C12M1/00 ,  C12M1/24 ,  C12M1/36

CPC分类号： C12M41/34 ,  C12M23/08 ,  C12M23/28 ,  C12M23/38 ,  C12M41/26 ,  C12M41/48

摘要： A disposable sensor cap (100) configured to attach to a cell culture device comprises a plurality of sensors for measuring cell culture media conditions. For example, the plurality of sensors (180) may measure dissolved gases and essential molecules of choice without the risk of contamination of the vessel. The sensors may be integrated in a body (150) of the sensor cap. During the measurement period, a small volume of cell culture media may be captured in a sampling chamber of the sensor cap when the cell culture vessel is tipped. An inductor coil (160) on the sensor cap allows for noninvasive wireless reading of the measurements.

公开(公告)号：US20240132822A1

公开(公告)日：2024-04-25

申请号：US18401976

申请日：2024-01-02

申请人： Terumo Kabushiki Kaisha ,  Terumo BCT, Inc.

发明人： Masatsugu IGARASHI ,  Thomas J. Felt

IPC分类号： C12M1/12 ,  C12M1/34 ,  C12M3/00

CPC分类号： C12M25/10 ,  C12M23/48 ,  C12M37/00 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34

摘要： A cell culturing system includes a cell culturing device and a support device. The cell culturing device cultures cells by allowing a culture medium inside circulation flow paths connected to the bioreactor to flow inside the bioreactor. The cell culturing device is connected to the circulation flow paths and includes a waste liquid flow path in order to discard the liquid flowing through the circulation flow paths, and a waste liquid accommodation unit in which the liquid guided from the waste liquid flow path is accommodated. A sampling unit, in which the culture medium that is guided from the circulation flow paths to the waste liquid flow path is collected, is connected to the waste liquid flow path.

公开(公告)号：US11965153B2

公开(公告)日：2024-04-23

申请号：US17517422

申请日：2021-11-02

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Matthew Angelini ,  Ashley Witmer ,  Anthony Debiase

IPC分类号： C12M1/34 ,  C12M1/00

CPC分类号： C12M41/46 ,  C12M21/14 ,  C12M29/04 ,  C12M29/10 ,  C12M41/14 ,  C12M41/26 ,  C12M41/30 ,  C12M41/32 ,  C12M41/38 ,  C12M43/00

摘要： A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

公开(公告)号：US20240110159A1

公开(公告)日：2024-04-04

申请号：US18535216

申请日：2023-12-11

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Donald E. INGBER ,  Hyun Jung Kim

IPC分类号： C12N5/071 ,  C12M1/00 ,  C12M1/06 ,  C12M1/12 ,  C12M1/24 ,  C12M1/34 ,  C12M1/42 ,  C12M3/00 ,  C12N1/14 ,  C12N1/20 ,  C12N5/074

CPC分类号： C12N5/0697 ,  C12M21/08 ,  C12M23/08 ,  C12M23/20 ,  C12M23/26 ,  C12M23/34 ,  C12M23/38 ,  C12M25/02 ,  C12M27/02 ,  C12M29/04 ,  C12M29/06 ,  C12M35/04 ,  C12M35/08 ,  C12M41/12 ,  C12M41/26 ,  C12M41/40 ,  C12M41/44 ,  C12N1/14 ,  C12N1/20 ,  C12N5/0679 ,  C12N5/068 ,  C12N5/0696

摘要： The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.

公开(公告)号：US11939559B2

公开(公告)日：2024-03-26

申请号：US17148190

申请日：2021-01-13

申请人： Genentech, Inc.

发明人： Dinesh Baskar ,  Jenny Hsiung ,  Woon-Lam Susan Leung ,  Inn H. Yuk

IPC分类号： C12M1/00 ,  C12M1/34 ,  C12M1/36 ,  C12M3/06 ,  C12N5/00

CPC分类号： C12M23/14 ,  C12M23/28 ,  C12M27/16 ,  C12M29/10 ,  C12M29/20 ,  C12M41/12 ,  C12M41/26 ,  C12M41/32 ,  C12M41/48 ,  C12N5/0018 ,  C12N2500/05

摘要： An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

公开(公告)号：US11884906B1

公开(公告)日：2024-01-30

申请号：US18116550

申请日：2023-03-02

申请人： University of South Florida

发明人： Subhra Mohapatra ,  Shyam S. Mohapatra ,  Tao Wang

IPC分类号： C12M3/06 ,  B01L3/00 ,  C12M1/32 ,  C12M1/12 ,  G01N33/50 ,  C12M1/34 ,  C12N5/09

CPC分类号： C12M23/16 ,  B01L3/502715 ,  C12M23/12 ,  C12M25/14 ,  C12M41/26 ,  C12M41/36 ,  C12N5/0693 ,  G01N33/5011 ,  B01L2300/0627 ,  B01L2300/0819 ,  B01L2300/0829 ,  C12N2503/02 ,  C12N2513/00

摘要： A 4D-perfused tumoroid-on-a-chip platform used in personalized cancer treatment. The platform includes a plate with a plurality of bottomless wells that resides atop a microfluidic channel layer, which in turn resides atop a surface acoustic wave (SAW) based sensor layer that is capable of measuring potential pH values of fluids disposed within the platform. The microfluidic channel layer includes a plurality of bioreactors, with each bioreactor including an inlet well, a culture well, and an outlet well. The inlet well, culture well, and outlet well form a closed system via fluid conduits spanning from the inlet well to the culture well, as well as from the culture well to the outlet well. Due to the fluid flow from the plate to the chip, and from the inlet well to the outlet well on the chip through the culture well, target cell (tumoroid) growth is promoted within the culture well.

公开(公告)号：US20240002771A1

公开(公告)日：2024-01-04

申请号：US18039365

申请日：2021-11-19

申请人： CORNING INCORPORATED

发明人： Stephen Joseph Caracci ,  Joel Andrew Schultes

IPC分类号： C12M1/00 ,  C12M1/34

CPC分类号： C12M29/26 ,  C12M29/10 ,  C12M41/12 ,  C12M41/26 ,  C12M41/34 ,  C12M29/06

摘要： A media conditioning vessel is provided for a perfusion bioreactor system. The media conditioning vessel includes a vessel having an interior cavity to hold a volume of liquid cell culture media; a media inlet to return cell culture media from a perfusion bioreactor to the vessel; and a media outlet to transfer cell culture media out of the vessel to the perfusion bioreactor. Sensors to measure or detect a characteristic of the cell culture media are provided in the form of inline sensors in at least one of the media inlet and the media outlet or patch sensors attached to a sidewall or bottom of the media conditioning vessel.