专利检索 ap:("Shengjun An" OR "Xiqing Chai" OR "Kunsheng Wang" OR "Tiemei Shao" OR "Zhan Jiao" OR "Xin Wen" OR "Xue Li" OR "Pei Liu" OR "Haigang Lu" OR "Yunyu Chen" OR "Liangyuan Hu" OR "Haimin Xu" OR "Chenggang Yu") AND inv:"Liangyuan Hu" 第 1 页

1.

发明授权
Seed-specific expression vector and its construction methods and applications 有权

公开(公告)号：US09745362B2

公开(公告)日：2017-08-29

申请号：US13515209

申请日：2010-12-09

申请人： Shengjun An , Xiqing Chai , Kunsheng Wang , Tiemei Shao , Zhan Jiao , Xin Wen , Xue Li , Pei Liu , Haigang Lu , Yunyu Chen , Liangyuan Hu , Haimin Xu , Chenggang Yu

发明人： Shengjun An , Xiqing Chai , Kunsheng Wang , Tiemei Shao , Zhan Jiao , Xin Wen , Xue Li , Pei Liu , Haigang Lu , Yunyu Chen , Liangyuan Hu , Haimin Xu , Chenggang Yu

IPC分类号： C07K14/775 , C12N15/82

CPC分类号： C07K14/775 , C12N15/8257

摘要： A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of Arachis hypogaea oleosin gene-apolipopoprotein A-IMilano (A-IM) gene driven by Brassica napus oleosin gene promoter is inserted between the HindIII and SacI sites of a plant binary expression vector pBI121, obtaining the plant expression vector pBINOA of the invention. In addition, a method for producing apolipoprotein A-IMilano is provided, in which the expression vector is used to transform oil sunflower which is used as a plant bioreactor. The method can not only improve the yield of apolipoprotein A-IMilano, but also greatly reduce production costs, and is suitable for industrial production.