公开(公告)号：US20170050944A1

公开(公告)日：2017-02-23

申请号：US15202298

申请日：2016-07-05

申请人： Synthetic Genomics, Inc.

发明人： Spiros Kambourakis ,  Benjamin M. Griffin

IPC分类号： C07D307/68 ,  C12P17/04

CPC分类号： C07D307/68 ,  C12P7/58 ,  C12P17/04

摘要： The present invention provides methods of method of synthesizing 2,5-furan dicarboxylic acid (FDCA) and FDCA precursor molecules. The methods involve performing a chemical dehydration reaction on a gluconic acid derivative in the presence of a dehydration catalyst. In some embodiments the gluconic acid derivative can be 2-dehydro-3-deoxy gluconic acid (DHG) or an ester thereof, 2-ketogluconic acid (2KGA) or an ester thereof, and 5-ketogluconic acid (5KGA) or an ester thereof. The 2,5-furan dicarboxylic acid precursor molecule is thereby synthesized, which can be converted into FDCA. The chemical dehydration can be performed by a variety of acid basic catalysts.

摘要翻译： 本发明提供了合成2,5-呋喃二羧酸（FDCA）和FDCA前体分子的方法。 所述方法包括在脱水催化剂存在下在葡萄糖酸衍生物上进行化学脱水反应。 在一些实施方案中，葡萄糖酸衍生物可以是2-脱氢-3-脱氧葡萄糖酸（DHG）或其酯，2-酮基葡萄糖酸（2KGA）或其酯，和5-酮基葡萄糖酸（5KGA）或其酯 。 由此合成了2,5-呋喃二羧酸前体分子，可以将其转化为FDCA。 化学脱水可以通过各种酸性碱性催化剂进行。

公开(公告)号：US09506090B2

公开(公告)日：2016-11-29

申请号：US14033300

申请日：2013-09-20

申请人： SYNTHETIC GENOMICS, INC.

发明人： Spiros Kambourakis ,  Benjamin M. Griffin ,  Kevin V. Martin

IPC分类号： C12P7/58 ,  C07D307/68 ,  C12P17/12

CPC分类号： C12P7/58 ,  C07D307/68 ,  C12P17/12

摘要： The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

摘要翻译： 本发明提供了生产一种或多种酶途径的产物的方法。 用于本发明方法的途径包括一个或多个转化步骤，例如将古罗糖醛酸酶促转化成D-葡糖二酸（步骤7）; 将5-酮葡萄糖酸（5-KGA）酶促转化成L-阿魏酸（步骤15）; 步骤7b）中L-赖康酸转化成Idaric酸的酶促转化; 并将5-酮基葡萄糖酸酯酶促转化成4,6-二羟基2,5-二酮己酸酯（2,5-DDH）（步骤16）。 在一些实施方案中，本发明的方法产生作为产物的2,5-呋喃二羧酸（FDCA）。 所述方法包括作为步骤的酶和化学转化。 还提供了将葡萄糖转化为5-脱氢-4-脱氧葡糖二酸（DDG）和将葡萄糖转化为2,5-呋喃二羧酸（FDCA）的各种途径。 该方法还涉及使用以高特异性和高效率进行反应的工程化酶。 可以生产的其它产品包括代谢产物，例如但不限于古洛糖醛酸，L-艾杜糖醛酸，id酸，葡糖二酸。 可以使用葡萄糖作为底物或在本发明的任何方法或途径中使用任何中间体来生产任何产物。

公开(公告)号：US20150176041A1

公开(公告)日：2015-06-25

申请号：US14548880

申请日：2014-11-20

申请人： Synthetic Genomics, Inc.

发明人： Benjamin M. Griffin ,  Spiros Kambourakis

IPC分类号： C12P7/64 ,  C12N9/02

CPC分类号： C12P7/6409 ,  C12N9/001 ,  C12N9/0071 ,  C12Y114/14001

摘要： The present invention provides methods of producing dicarboxylic acids. The methods involve incubating a fatty acid or hydrocarbon substrate with an enzyme to produce a dicarboxylic acid product. The enzyme acts on the substrate to produce a product that has been both over-oxidized and has undergone cleavage of a C—C bond. In some embodiments the enzymes having these useful characteristics are mutants of a cytochrome P450 enzyme, for example an enzyme of the class CYP102 or a mutant thereof. The invention provides enzymes where these desirable characteristics can be found in a single enzyme, and thus in some embodiments the methods can be performed through the action of a single enzyme.

摘要翻译： 本发明提供了生产二羧酸的方法。 所述方法包括用酶孵育脂肪酸或烃底物以产生二羧酸产物。 该酶作用在底物上以产生已经过度氧化并经历C-C键裂解的产物。 在一些实施方案中，具有这些有用特征的酶是细胞色素P450酶的突变体，例如CYP102类的酶或其突变体。 本发明提供了酶，其中这些所需特征可以在单一酶中发现，因此在一些实施方案中，可以通过单一酶的作用进行该方法。

公开(公告)号：US10087161B2

公开(公告)日：2018-10-02

申请号：US15202298

申请日：2016-07-05

申请人： Synthetic Genomics, Inc.

发明人： Spiros Kambourakis ,  Benjamin M. Griffin

IPC分类号： C07D307/46 ,  C07D307/40 ,  C07D307/68 ,  C12P17/04 ,  C12P7/58

摘要： The present invention provides methods of method of synthesizing 2,5-furan dicarboxylic acid (FDCA) and FDCA precursor molecules. The methods involve performing a chemical dehydration reaction on a gluconic acid derivative in the presence of a dehydration catalyst. In some embodiments the gluconic acid derivative can be 2-dehydro-3-deoxy gluconic acid (DHG) or an ester thereof, 2-ketogluconic acid (2KGA) or an ester thereof, and 5-ketogluconic acid (5KGA) or an ester thereof. The 2,5-furan dicarboxylic acid precursor molecule is thereby synthesized, which can be converted into FDCA. The chemical dehydration can be performed by a variety of acid basic catalysts.

公开(公告)号：US20140206047A1

公开(公告)日：2014-07-24

申请号：US14222453

申请日：2014-03-21

申请人： Synthetic Genomics, Inc.

发明人： Spiros Kambourakis ,  Benjamin M. Griffin ,  Kevin V. Martin

IPC分类号： C12P7/58 ,  C12P7/42 ,  C12P17/04

CPC分类号： C12P7/58 ,  C07D307/33 ,  C07D309/30 ,  C12N15/52 ,  C12P7/42

摘要： The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

摘要翻译： 本发明提供了生产一种或多种酶途径的产物的方法。 用于本发明方法的途径包括一个或多个转化步骤，例如将古罗糖醛酸酶促转化成D-葡糖二酸（步骤7）; 将5-酮葡萄糖酸（5-KGA）酶促转化成L-阿魏酸（步骤15）; 步骤7b）中将L-阿魏酸转化成Idaric酸的酶促转化; 并将5-酮基葡萄糖酸酯酶促转化成4,6-二羟基2,5-二酮己酸酯（2,5-DDH）（步骤16）。 在一些实施方案中，本发明的方法产生作为产物的2,5-呋喃二羧酸（FDCA）。 所述方法包括作为步骤的酶和化学转化。 还提供了将葡萄糖转化为5-脱氢-4-脱氧葡糖二酸（DDG）和将葡萄糖转化为2,5-呋喃二羧酸（FDCA）的各种途径。 该方法还涉及使用以高特异性和高效率进行反应的工程化酶。 可以生产的其它产品包括代谢产物，例如但不限于古洛糖醛酸，L-艾杜糖醛酸，id酸，葡糖二酸。 可以使用葡萄糖作为底物或在本发明的任何方法或途径中使用任何中间体来生产任何产物。

公开(公告)号：US09909154B2

公开(公告)日：2018-03-06

申请号：US14548880

申请日：2014-11-20

申请人： Synthetic Genomics, Inc.

发明人： Benjamin M. Griffin ,  Spiros Kambourakis

IPC分类号： C12P7/64 ,  C12N9/02

CPC分类号： C12P7/6409 ,  C12N9/001 ,  C12N9/0071 ,  C12Y114/14001

摘要： The present invention provides methods of producing dicarboxylic acids. The methods involve incubating a fatty acid or hydrocarbon substrate with an enzyme to produce a dicarboxylic acid product. The enzyme acts on the substrate to produce a product that has been both over-oxidized and has undergone cleavage of a C—C bond. In some embodiments the enzymes having these useful characteristics are mutants of a cytochrome P450 enzyme, for example an enzyme of the class CYP102 or a mutant thereof. The invention provides enzymes where these desirable characteristics can be found in a single enzyme, and thus in some embodiments the methods can be performed through the action of a single enzyme.

公开(公告)号：US20170088865A1

公开(公告)日：2017-03-30

申请号：US15377749

申请日：2016-12-13

申请人： Synthetic Genomics, Inc.

发明人： Spiros Kambourakis ,  Benjamin M. Griffin ,  Kevin V. Martin

IPC分类号： C12P7/58 ,  C12P7/42

CPC分类号： C12P7/58 ,  C07D307/33 ,  C07D309/30 ,  C12N15/52 ,  C12P7/42

摘要： The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid.

公开(公告)号：US09528133B2

公开(公告)日：2016-12-27

申请号：US14222453

申请日：2014-03-21

申请人： SYNTHETIC GENOMICS, INC.

发明人： Spiros Kambourakis ,  Benjamin M. Griffin ,  Kevin V. Martin

IPC分类号： C12P7/58 ,  C12P7/42 ,  C12P17/04 ,  C07D309/30 ,  C07D307/33 ,  C12N15/52

CPC分类号： C12P7/58 ,  C07D307/33 ,  C07D309/30 ,  C12N15/52 ,  C12P7/42

摘要： The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

摘要翻译： 本发明提供了生产一种或多种酶途径的产物的方法。 用于本发明方法的途径包括一个或多个转化步骤，例如将古罗糖醛酸酶促转化成D-葡糖二酸（步骤7）; 将5-酮葡萄糖酸（5-KGA）酶促转化成L-阿魏酸（步骤15）; 步骤7b）中L-赖康酸转化成Idaric酸的酶促转化; 并将5-酮基葡萄糖酸酯酶促转化成4,6-二羟基2,5-二酮己酸酯（2,5-DDH）（步骤16）。 在一些实施方案中，本发明的方法产生作为产物的2,5-呋喃二羧酸（FDCA）。 所述方法包括作为步骤的酶和化学转化。 还提供了将葡萄糖转化为5-脱氢-4-脱氧葡糖二酸（DDG）和将葡萄糖转化为2,5-呋喃二羧酸（FDCA）的各种途径。 该方法还涉及使用以高特异性和高效率进行反应的工程化酶。 可以生产的其它产品包括代谢产物，例如但不限于古洛糖醛酸，L-艾杜糖醛酸，id酸，葡糖二酸。 可以使用葡萄糖作为底物或在本发明的任何方法或途径中使用任何中间体来生产任何产物。