公开(公告)号：US3919049A

公开(公告)日：1975-11-11

申请号：US48389674

申请日：1974-06-27

申请人： TOKYO TANABE CO

发明人： KIUCHI AKIRA ,  TANAKA YUJI

IPC分类号： C12N9/38 ,  C12D13/10

CPC分类号： C12Y302/01023 ,  C12N9/2471 ,  Y10S435/814 ,  Y10S435/815 ,  Y10S435/917 ,  Y10S435/918

摘要： An acid-active, acid-stable Beta -galactosidase is prepared from the cultured material of a strain of Aspergillus oryzae. Said enzyme is recovered, in case of the liquid culture, by means of precipitation from the cultured broth by adding organic solvent thereto, while in case of the solid culture, the enzyme is recovered from the cultured material by means of extraction using water and succeeding precipitation from the resulting aqueous solution by adding organic solvent thereto. The precipitates thus obtained in both cases may further be refined in the routine manner. The present acid-active, acid-stable Beta -galactosidase is useful for recovering or preventing of vomit or diarrhoea due to the Beta -galactosidase deficiency or lactose intolerance.

摘要翻译： 从米曲霉菌株的培养物质制备酸活性，酸稳定的β-半乳糖苷酶。 在液体培养的情况下，通过加入有机溶剂从培养液中沉淀回收所述酶，而在固体培养的情况下，通过使用水萃取从培养物质中回收酶 通过向其中加入有机溶剂从所得水溶液中沉淀。 这两种情况下得到的沉淀物可以进一步以常规方式进行精制。 本发明的酸活性，酸稳定的β-半乳糖苷酶可用于恢复或预防由于β-半乳糖苷酶缺乏或乳糖不耐受引起的呕吐或腹泻。