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133 条记录 (耗时 0.017 秒)

    Immobilized ribonuclease and alkaline phosphatase
    4.
    发明授权
    Immobilized ribonuclease and alkaline phosphatase 失效
    固定核糖核酸酶和碱性磷酸酶

    公开(公告)号：US4039382A

    公开(公告)日：1977-08-02

    申请号：US678459

    申请日：1976-04-19

    申请人： Minh-Nguy Thang ,  Annie Guissani born Trachtenberg

    发明人： Minh-Nguy Thang ,  Annie Guissani born Trachtenberg

    IPC分类号： C12N11/12 ,  C07H21/00 ,  C12N11/14 ,  C12N11/18 ,  C12P19/34 ,  C07G7/02 ,  C12B1/00

    CPC分类号： C07H21/00 ,  C12N11/18 ,  C12P19/34 ,  Y10S435/918

    摘要： An insoluble, solid matrix carrying simultaneously several different enzymatic functions, is constituted by the conjoint association by irreversible binding on a previously activated matrix support, of a nuclease selected from the group of ribonucleases A, T.sub.1, T.sub.2, U.sub.2 and an alkaline phosphatease. Free activated groups of the matrix after binding of the enzymes, are neutralized by a free amino organic base. The support is selected from among non-denaturing supports effecting the irreversible physical adsorption of the enzymes, such as supports of glass or quartz beads, highly cross-linked gels of the agarose or cellulose type. Polymers A.sub.n U.sub.OH, A.sub.n C.sub.OH, A.sub.n G.sub.OH and/or oligonucleotides U, C, A or G, of predetermined lengths, can be obtained by fractionating the polyribonucleotides by incubation with the insoluble solid matrix.

    摘要翻译： 携带同时几种不同酶功能的不溶性固体基质是由预先活化的基质载体，选自核糖核酸酶A，T1，T2，U2和碱性磷酸酶的核酸酶的不可逆结合的共同关联构成的。 酶结合后，自由基活化基团被游离氨基有机碱中和。 支持物选自影响酶的不可逆物理吸附的非变性支持物，例如玻璃或石英珠的支撑物，琼脂糖或纤维素类型的高度交联的凝胶。 具有预定长度的聚合物AnUOH，AnCOH，AnGOH和/或寡核苷酸U，C，A或G可以通过与不溶性固体基质孵育来分级多核糖核苷酸而获得。

    Integral element for the analysis of cholesterol
    5.
    发明授权
    Integral element for the analysis of cholesterol 失效
    胆固醇分析的积分要素

    公开(公告)号：US3983005A

    公开(公告)日：1976-09-28

    申请号：US565897

    申请日：1975-04-07

    申请人： Charles T. Goodhue ,  Hugh A. Risley ,  Roy E. Snoke ,  Gary M. Underwood

    发明人： Charles T. Goodhue ,  Hugh A. Risley ,  Roy E. Snoke ,  Gary M. Underwood

    IPC分类号： C12Q1/60 ,  C12K1/04

    CPC分类号： C12Q1/60 ,  Y10S435/805 ,  Y10S435/839 ,  Y10S435/897 ,  Y10S435/918

    摘要： An integral analytical element for analysis of liquids for their cholesterol content is disclosed. The element is of the type which comprises at least two superposed layers including a spreading layer and a reagent layer in fluid contact and, optionally, a support. Cholesterol oxidase and a composition for the hydrolysis of cholesterol esters comprising lipase having cholesterol esterase activity and protease are included in the element such that cholesterol esters contained in a sample applied to the spreading layer are saponified to free cholesterol and free cholesterol is decomposed in the presence of cholesterol oxidase to produce a detectable change related to the total cholesterol content of the sample.

    摘要翻译： 公开了用于分析其胆固醇含量的液体的整体分析元件。 该元件是包括至少两个叠加层的类型，包括扩散层和流体接触的试剂层，以及任选的支撑体。 胆固醇氧化酶和用于水解包含具有胆固醇酯酶活性的脂肪酶的胆固醇酯和蛋白酶的组合物包括在元件中，使得包含在施加到铺展层上的样品中的胆固醇酯被皂化以游离胆固醇并且游离胆固醇在存在下分解 的胆固醇氧化酶以产生与样品的总胆固醇含量相关的可检测的变化。

    Process for preparing {62 -galactosidase
    6.
    发明授权
    Process for preparing {62 -galactosidase 失效
    制备{62-半乳糖苷酶的方法

    公开(公告)号：US3919049A

    公开(公告)日：1975-11-11

    申请号：US48389674

    申请日：1974-06-27

    申请人： TOKYO TANABE CO

    发明人： KIUCHI AKIRA ,  TANAKA YUJI

    IPC分类号： C12N9/38 ,  C12D13/10

    CPC分类号： C12Y302/01023 ,  C12N9/2471 ,  Y10S435/814 ,  Y10S435/815 ,  Y10S435/917 ,  Y10S435/918

    摘要： An acid-active, acid-stable Beta -galactosidase is prepared from the cultured material of a strain of Aspergillus oryzae. Said enzyme is recovered, in case of the liquid culture, by means of precipitation from the cultured broth by adding organic solvent thereto, while in case of the solid culture, the enzyme is recovered from the cultured material by means of extraction using water and succeeding precipitation from the resulting aqueous solution by adding organic solvent thereto. The precipitates thus obtained in both cases may further be refined in the routine manner. The present acid-active, acid-stable Beta -galactosidase is useful for recovering or preventing of vomit or diarrhoea due to the Beta -galactosidase deficiency or lactose intolerance.

    摘要翻译： 从米曲霉菌株的培养物质制备酸活性，酸稳定的β-半乳糖苷酶。 在液体培养的情况下，通过加入有机溶剂从培养液中沉淀回收所述酶，而在固体培养的情况下，通过使用水萃取从培养物质中回收酶 通过向其中加入有机溶剂从所得水溶液中沉淀。 这两种情况下得到的沉淀物可以进一步以常规方式进行精制。 本发明的酸活性，酸稳定的β-半乳糖苷酶可用于恢复或预防由于β-半乳糖苷酶缺乏或乳糖不耐受引起的呕吐或腹泻。

    Method for preparing alpha-amylase
    7.
    发明授权
    Method for preparing alpha-amylase 失效
    制备α-淀粉酶的方法

    公开(公告)号：US3826716A

    公开(公告)日：1974-07-30

    申请号：US34678573

    申请日：1973-04-02

    申请人： KVESITADZE G ,  KOKONASHVILI G ,  FENIXOVA R

    发明人： KOKONASHVILI G ,  FENIXOVA R ,  KVESITADZE G

    IPC分类号： C12N9/30 ,  C12D13/10

    CPC分类号： C12N9/242 ,  Y10S435/815 ,  Y10S435/918

    摘要： A METHOD FOR PREPARING A-AMYLASE BY DEPTH CULTIVATION OF ASPERGILLUS ORYZAE ON A WATER NUTRIENT MEDIUM CONTAINING THE FOLLOWING INGREDIENT: STARCH, NANO3, MGSO4, KCL, FESO4, KH2PO4, MG(NO3)2, MG(H2PO4)2 AND 20 PERCENT BY VOLUME OF 20 PERCENT EXTRACT OF MALT SPROUTS. THE CULTURE FILTRATE IS DIALYZED WITH PHOSPHATE BUFFER AT PH 6.5-7.5. FORM THE DIALYZATE, THE A-AMYLASE IS ADSORBED ON DIETHYLAMINE ETHYLCELLULOSE AND THEN ELUTED WITH A PHOSPHATE BUFFER (FROM 0.04 TO 0.12M) AT PH 6.5-7.5 CONTAINING CACL2 (0.0003-0.001M).