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    Subcutaneous Glucose Electrode
    1.
    发明申请
    Subcutaneous Glucose Electrode 审中-公开

    公开(公告)号：US20100121166A1

    公开(公告)日：2010-05-13

    申请号：US12551222

    申请日：2009-08-31

    申请人： Adam Heller ,  Michael V. Pishko

    发明人： Adam Heller ,  Michael V. Pishko

    IPC分类号： A61B5/1473

    CPC分类号： C12Q1/54 ,  C12Q1/006 ,  Y10S435/817 ,  Y10S435/917 ,  Y10S435/962

    摘要： A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow “one-point” in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.

    Subcutaneous Glucose Electrode
    2.
    发明申请
    Subcutaneous Glucose Electrode 审中-公开

    公开(公告)号：US20100072063A1

    公开(公告)日：2010-03-25

    申请号：US12551510

    申请日：2009-08-31

    申请人： Adam Heller ,  Michael V. Pishko

    发明人： Adam Heller ,  Michael V. Pishko

    IPC分类号： G01N27/327

    CPC分类号： C12Q1/54 ,  C12Q1/006 ,  Y10S435/817 ,  Y10S435/917 ,  Y10S435/962

    摘要： A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow “one-point” in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.

    Subcutaneous Glucose Electrode
    3.
    发明申请
    Subcutaneous Glucose Electrode 审中-公开

    公开(公告)号：US20100051479A1

    公开(公告)日：2010-03-04

    申请号：US12615009

    申请日：2009-11-09

    申请人： Adam Heller ,  Michael V. Pishko

    发明人： Adam Heller ,  Michael V. Pishko

    IPC分类号： G01N33/50

    CPC分类号： C12Q1/54 ,  C12Q1/006 ,  Y10S435/817 ,  Y10S435/917 ,  Y10S435/962

    摘要： A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow “one-point” in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.

    METHOD OF DETERMINING ANALYTE LEVEL USING SUBCUTANEOUS ELECTRODE
    4.
    发明申请
    METHOD OF DETERMINING ANALYTE LEVEL USING SUBCUTANEOUS ELECTRODE 审中-公开
    使用细胞电极测定分析物水平的方法

    公开(公告)号：US20050287620A1

    公开(公告)日：2005-12-29

    申请号：US11109379

    申请日：2005-04-19

    申请人： Adam Heller ,  Michael Pishko

    发明人： Adam Heller ,  Michael Pishko

    IPC分类号： G01N27/327 ,  A61B5/145 ,  A61B5/1473 ,  A61B5/1486 ,  A61B5/1495 ,  C12M1/40 ,  C12Q1/00 ,  C12Q1/54

    CPC分类号： C12Q1/54 ,  C12Q1/006 ,  Y10S435/817 ,  Y10S435/917 ,  Y10S435/962

    摘要： A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow “one-point” in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.

    摘要翻译： 描述了设计用于皮下体内电导率监测葡萄糖的小直径柔性电极。 电极被设计成允许“一点”体内校准，即即使在血清或血液的其它电反应物质的存在下也能在零葡萄糖浓度下具有零输出电流。 电极优选为三层或四层，其中层叠在聚酰胺绝缘金线的尖端上的凹槽内。 第一葡萄糖浓度 - 电流转导层用电绝缘和葡萄糖通量限制层（第二层）涂覆，其上任选地沉积固定的干扰消除辣根过氧化物酶基膜（第三层）。 外（第四）层是生物相容的。

    Process for preparing beta-fructofuranosidase enzyme and a process for producing fructooligosaccharides
    7.
    发明申请
    Process for preparing beta-fructofuranosidase enzyme and a process for producing fructooligosaccharides 失效
    制备β-呋喃果糖苷酶的方法和一种生产低聚果糖的方法

    公开(公告)号：US20020182682A1

    公开(公告)日：2002-12-05

    申请号：US10196440

    申请日：2002-07-15

    发明人： Yong Kun Park ,  Glaucia Maria Pastores

    IPC分类号： C12N015/09

    CPC分类号： C12N9/2408 ,  C12P19/18 ,  Y10S435/814 ,  Y10S435/913 ,  Y10S435/917

    摘要： A process for preparing beta-fructofuranosidase enzyme and a process for producing fructooligosaccharides, in which the preparation of the enzyme is obtained by cultivating the fungus Aspergillus niger, either wild or mutated, in a preferably semi-solid culture medium, in order to produce an extracellular enzyme, which is submitted to transfructosylation for producing fructooligosaccharides comprising sugars which are formed by one unit of sacrose and by two, three and four units of fructose.

    摘要翻译： 制备β-呋喃果糖苷酶的方法和制备低聚果糖的方法，其中通过在优选半固体培养基中培养野生或突变的真菌黑曲霉获得酶的制备，以产生 细胞外酶，其被转葡糖基化以产生包含由一个单位的糖和由两个，三个和四个单位的果糖形成的糖的低聚果糖。

    Protein with phospholipase activity
    10.
    发明授权
    Protein with phospholipase activity 有权
    具有磷脂酶活性的蛋白质

    公开(公告)号：US6140094A

    公开(公告)日：2000-10-31

    申请号：US142469

    申请日：1998-09-08

    申请人： Fridolin Loffler ,  Gerald Jungschaffer ,  Quoc Nguyen Khanh ,  Erwin Schuster ,  Bruno Sprossler ,  Sabine Wolf

    发明人： Fridolin Loffler ,  Gerald Jungschaffer ,  Quoc Nguyen Khanh ,  Erwin Schuster ,  Bruno Sprossler ,  Sabine Wolf

    IPC分类号： A21D2/26 ,  A21D8/04 ,  C11B3/00 ,  C12N1/15 ,  C12N9/18 ,  C12S3/18 ,  C12N9/20 ,  C07H21/04 ,  C07K1/00 ,  C12N1/20 ,  C12N15/00

    CPC分类号： A21D8/042 ,  A21D2/267 ,  C11B3/003 ,  C12N9/18 ,  Y10S435/913 ,  Y10S435/917

    摘要： This invention relates to a protein having phospholipase activity, which is characterised in that it has the mature sequence of Aspergillus lysophospholipase or a sequence derived therefrom and that it may be cleaved at at least one site, wherein, in the event of cleavage, the restriction fragments are optionally either linked by means of at least one bond cleavable under reducing conditions or at least one of the unlinked restriction fragments has phospholipase activity, and to a process for the production of this protein by fermenting a suitably transformed lysophospholipase-producing host organism in a suitable culture medium and isolating the protein having phospholipase activity from the cell-free culture filtrate, wherein fermentation is performed in the acidic to slightly alkaline range.

    摘要翻译： PCT No.PCT / EP98 / 00081 Sec。 371日期：1998年9月8日 102（e）1998年9月8日PCT PCT 1998年1月8日PCT公布。 公开号WO98 / 31790 日期1998年7月23日本发明涉及具有磷脂酶活性的蛋白质，其特征在于其具有成熟的曲霉溶血磷脂酶序列或其衍生的序列，并且其可在至少一个位点断裂，其中，在事件 的切割，限制性片段任选地通过至少一个在还原条件下可切割的键连接或至少一个未连接的限制性片段具有磷脂酶活性，以及​​通过发酵适当转化的溶血磷脂酶来生产该蛋白质的方法 - 在合适的培养基中产生宿主生物体，并从无细胞培养滤液中分离具有磷脂酶活性的蛋白质，其中发酵在酸性至略微碱性范围内进行。